FastQCFastQC Report
Fri 10 Feb 2017
ERR1630933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255168
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG159856.26450025081515No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT136855.363133308251818TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT94713.7116723100075246No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC85003.3311387007775273No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC51072.001426511161274RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC42601.6694883370955607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19020.7453912716328066No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG18710.7332424128417356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15830.6203755956859794No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA13960.5470905442688739No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT13540.530630800100326No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT10560.41384499623777277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6560.2570855279658891No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6390.25042325056433407No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4920.19281414597441685No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT4350.17047592174567344No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4270.16734073238023575No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4130.16185415099071984No Hit
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT3720.14578630549285176TruSeq Adapter, Index 11 (95% over 23bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3480.13638073739653875No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA3140.12305618259342865No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3060.11992099322799096No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2940.11521820917983445No Hit
CTGATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT2780.10894783044895912No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2730.10698833709556056No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2710.10620453975420116No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2710.10620453975420116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGG200.001839253437.01
CCTACGT200.001839253437.032
CTTATAC12100.034.706611
TTATACA12700.032.9212572
GCAACGC451.0743679E-732.888893
CGTCTTC6500.032.16153737
TTTAACG352.3810844E-531.71428734
TTAACGG352.3810844E-531.71428735
GGGAGCC303.5917034E-430.8333327
CCCACGC303.5917034E-430.83333219
TATACAC14350.029.6515673
AGCAACG502.7067654E-729.62
ATGTCAT250.005488431529.67
GCACTAG250.005488431529.630
AATGTCA250.005488431529.66
TGATCTC250.005488431529.618
CACATGT405.9223064E-527.75000228
AACGGCC405.9223064E-527.75000237
ACATGTT405.9223064E-527.75000229
CATGTTT405.9223064E-527.75000230
Produced by FastQC (version 0.11.5)