FastQCFastQC Report
Fri 10 Feb 2017
ERR1630932.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630932.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754986
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG38460.5094134195865884No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT37080.4911349349524362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.4525911738760719No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33620.44530627057985184No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT29230.3871594969972953No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC25260.33457574047730687No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA16480.2182821933121939No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC13180.1745727735348735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10410.13788335147936517No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC8630.11430675535705297Illumina PCR Primer Index 7 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4800.029.2916661
CGTCTTC1650.026.9090937
TTTACCG358.866563E-426.42857230
GTTACGG358.866563E-426.42857217
CTTATAC6200.025.3629021
GTATCAA14450.023.8131481
CCGTCTT4150.023.62650337
GCCGTCT4200.023.34523836
TCGAGGT400.001930576623.12515
CGCCGTT400.001930576623.12525
TTACCGG400.001930576623.12531
GATTACG400.001930576623.1251
CCGTTTA400.001930576623.12527
TACCGGG400.001930576623.12532
TATACAC7050.022.8297863
TTATACA6950.022.0935232
GTCGCCC957.1358954E-921.42105337
GCTCGGA957.1358954E-921.42105311
GGACCGT705.0980943E-621.1428596
CCGCCGT806.9536145E-720.812524
Produced by FastQC (version 0.11.5)