##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630932.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	754986
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.581214751002005	31.0	31.0	34.0	30.0	34.0
2	31.57956836285706	31.0	31.0	34.0	30.0	34.0
3	31.066144272873935	31.0	31.0	34.0	27.0	34.0
4	35.19625794385591	37.0	35.0	37.0	32.0	37.0
5	35.395445743364775	37.0	35.0	37.0	33.0	37.0
6	35.58564529673398	37.0	35.0	37.0	33.0	37.0
7	35.59771174564826	37.0	35.0	37.0	33.0	37.0
8	35.62296916764019	37.0	35.0	37.0	33.0	37.0
9	37.389991337587716	39.0	37.0	39.0	34.0	39.0
10	37.24301377773892	39.0	37.0	39.0	34.0	39.0
11	37.30685204758764	39.0	37.0	39.0	34.0	39.0
12	37.243995252892105	39.0	37.0	39.0	34.0	39.0
13	37.31130113670982	39.0	37.0	39.0	34.0	39.0
14	38.49534560905765	40.0	38.0	41.0	34.0	41.0
15	38.50042119986331	40.0	38.0	41.0	34.0	41.0
16	38.51174061505776	40.0	38.0	41.0	34.0	41.0
17	38.502215935129925	40.0	38.0	41.0	34.0	41.0
18	38.530004529885325	40.0	38.0	41.0	34.0	41.0
19	38.56586347296506	40.0	38.0	41.0	34.0	41.0
20	38.55408709565475	40.0	38.0	41.0	34.0	41.0
21	38.51902286929824	40.0	38.0	41.0	34.0	41.0
22	38.464109268251335	40.0	38.0	41.0	34.0	41.0
23	38.43277226332674	40.0	38.0	41.0	34.0	41.0
24	38.41271228870469	40.0	38.0	41.0	34.0	41.0
25	38.37926133729632	40.0	38.0	41.0	34.0	41.0
26	38.2805906334687	40.0	38.0	41.0	34.0	41.0
27	38.219964343709684	40.0	38.0	41.0	34.0	41.0
28	38.184312026977985	40.0	38.0	41.0	34.0	41.0
29	38.101209823758325	40.0	38.0	41.0	33.0	41.0
30	38.03035288071567	40.0	37.0	41.0	33.0	41.0
31	37.98730704940224	40.0	37.0	41.0	33.0	41.0
32	37.89253708015778	40.0	37.0	41.0	33.0	41.0
33	37.88651445192361	40.0	37.0	41.0	33.0	41.0
34	37.821754310675956	40.0	37.0	41.0	33.0	41.0
35	37.76794801493008	40.0	37.0	41.0	33.0	41.0
36	37.74982185100121	40.0	37.0	41.0	33.0	41.0
37	37.69580760437942	40.0	37.0	41.0	33.0	41.0
38	37.66326925267489	40.0	37.0	41.0	33.0	41.0
39	37.578512714143045	40.0	37.0	41.0	32.0	41.0
40	37.52998466196724	40.0	37.0	41.0	32.0	41.0
41	37.470064345564026	40.0	37.0	41.0	32.0	41.0
42	37.409561236897105	40.0	37.0	41.0	32.0	41.0
43	36.84982105628449	39.0	36.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	3.0
12	2.0
13	3.0
14	3.0
15	1.0
16	5.0
17	8.0
18	13.0
19	24.0
20	50.0
21	131.0
22	249.0
23	472.0
24	808.0
25	1361.0
26	2241.0
27	3634.0
28	5503.0
29	7752.0
30	10914.0
31	14551.0
32	19391.0
33	25567.0
34	35119.0
35	46951.0
36	66954.0
37	101958.0
38	180116.0
39	231195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.502568259543885	23.16334342623572	15.50743987305725	22.826648441163147
2	13.78409665874599	23.25579547170411	40.452670645548395	22.507437224001507
3	14.641198644743081	29.01947850688622	33.93003313968736	22.40928970868334
4	11.51518041394145	18.716506001435786	41.17056475219408	28.59774883242868
5	11.959427062223671	39.10403636623725	36.445841379840154	12.490695191698919
6	26.847120343953396	41.85216679514587	18.06708998577457	13.23362287512616
7	24.144288768268552	33.436249148990846	24.75436630613018	17.665095776610425
8	24.495156201571948	35.46953188535946	22.372732739415035	17.66257917365355
9	24.774366676997982	15.539228541986208	21.299202899126605	38.3872018818892
10	14.259734617595557	29.37140556248725	34.87521622917511	21.493643590742078
11	31.598864084896938	24.668669352809193	25.60338867210783	18.129077890186043
12	20.90634793227954	27.16407986373257	31.18614119996927	20.743431004018618
13	27.81018455971369	22.338559920316403	27.232690407504244	22.61856511246566
14	20.99416413019579	22.283062202477925	29.043452461370144	27.679321205956136
15	22.3021354038353	30.41274937548511	24.78456554161269	22.500549679066896
16	21.88557138807872	28.919344199759994	26.072139086022787	23.122945326138495
17	20.86012190954534	29.560283237040156	27.353487349434296	22.226107503980206
18	22.194186382264043	27.527530311820353	28.69271748085395	21.585565825061657
19	23.26480226123398	27.480244666788522	28.91232420203818	20.34262886993931
20	22.966121226088962	27.198517588405615	28.017870530049567	21.817490655455863
21	22.93790878241451	27.25004172262797	28.336551936062392	21.475497558895132
22	21.056152034607265	29.208356181439125	28.613510714105956	21.121981069847653
23	21.98186456437603	27.084078380261357	28.683843144111282	22.250213911251336
24	21.58940695589057	28.20899990198494	28.64039862990837	21.56119451221612
25	21.425695310906427	28.132574643768226	28.690333330684275	21.751396714641068
26	22.295645217262308	27.764223442553902	28.626226181677538	21.31390515850625
27	21.068735049391645	28.62794806791119	28.39562587915537	21.90769100354179
28	21.778019724869072	27.356268857965578	28.903847223657124	21.961864193508223
29	21.713515217500724	28.319730432087482	28.865700820942376	21.10105352946942
30	21.59629450082518	27.410177142357604	28.757089535435092	22.23643882138212
31	22.628763977080368	27.431237135523045	28.123435401451154	21.816563485945434
32	21.152180305330166	28.464368875714253	29.280542950465303	21.102907868490277
33	20.73720572302003	27.68634120367795	29.22305844081877	22.35339463248325
34	22.199484493752202	27.640247633730958	28.619206183955733	21.54106168856111
35	21.083437308771288	27.501172207166757	30.313542237869314	21.101848246192645
36	21.767555954679953	27.7574683504065	28.070321833782348	22.404653861131198
37	21.766628785169527	27.35467942451913	29.20358788109978	21.67510390921156
38	21.72040276243533	27.20447796382979	28.982391726469096	22.092727547265778
39	22.082926041012684	27.520112955736924	28.657485039457686	21.739475963792707
40	21.08688108123859	27.02328255093472	30.22612339831467	21.663712969512016
41	21.43536436437232	27.13017195020835	28.472448495733694	22.962015189685637
42	21.284897998108573	27.26500888758202	29.30478181052364	22.145311303785768
43	21.80080160426816	26.851623738718335	29.447036103980732	21.90053855303277
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	30.0
2	36.0
3	165.5
4	295.0
5	295.0
6	346.5
7	398.0
8	383.5
9	369.0
10	565.0
11	761.0
12	761.0
13	1200.0
14	1639.0
15	3806.0
16	5973.0
17	6705.5
18	7438.0
19	7438.0
20	7905.5
21	8373.0
22	10338.0
23	12303.0
24	15547.0
25	18791.0
26	18791.0
27	22327.5
28	25864.0
29	29493.0
30	33122.0
31	36671.0
32	40220.0
33	40220.0
34	43042.5
35	45865.0
36	48250.5
37	50636.0
38	52770.0
39	54904.0
40	54904.0
41	54866.5
42	54829.0
43	54741.0
44	54653.0
45	53981.0
46	53309.0
47	53309.0
48	52287.5
49	51266.0
50	48959.5
51	46653.0
52	47785.5
53	48918.0
54	48918.0
55	43158.0
56	37398.0
57	35284.5
58	33171.0
59	28690.0
60	24209.0
61	24209.0
62	20292.0
63	16375.0
64	13707.0
65	11039.0
66	9001.5
67	6964.0
68	6964.0
69	5657.5
70	4351.0
71	3616.0
72	2881.0
73	1992.0
74	1103.0
75	1103.0
76	779.0
77	455.0
78	345.5
79	236.0
80	166.5
81	97.0
82	97.0
83	69.0
84	41.0
85	29.5
86	18.0
87	12.5
88	7.0
89	7.0
90	4.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	754986.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.7183184113319
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6375474350827	45.73626091331901
2	11.77561587082204	13.35786260901636
3	3.197175193271362	5.440152018863302
4	1.2731296285575036	2.888390866057008
5	0.680982544095397	1.9312092384280801
6	0.4287594477897912	1.4591108928964929
7	0.30127194433721477	1.1961346647123445
8	0.21395508094119225	0.9708137925235866
9	0.15247789971551376	0.778346106008008
>10	1.1597211325591987	12.962388330179722
>50	0.11824926835761665	4.594950409260563
>100	0.05807053451636383	5.178886745692836
>500	9.366215243453296E-4	0.3461002399844464
>1k	0.0021073984297769912	3.159393173058325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG	3846	0.5094134195865884	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	3708	0.4911349349524362	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3417	0.4525911738760719	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3362	0.44530627057985184	No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	2923	0.3871594969972953	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	2526	0.33457574047730687	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1648	0.2182821933121939	No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	1318	0.1745727735348735	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1041	0.13788335147936517	No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC	863	0.11430675535705297	Illumina PCR Primer Index 7 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	2.6490557440800224E-4	0.0
19	0.0	0.0	0.0	2.6490557440800224E-4	0.0
20	0.0	0.0	0.0	2.6490557440800224E-4	0.0
21	0.0	0.0	0.0	5.298111488160045E-4	0.0
22	0.0	0.0	0.0	7.947167232240068E-4	0.0
23	0.0	0.0	0.0	0.0013245278720400114	0.0
24	0.0	0.0	0.0	0.001854339020856016	0.0
25	0.0	0.0	0.0	0.002119244595264018	0.0
26	0.0	0.0	0.0	0.002119244595264018	0.0
27	0.0	0.0	0.0	0.0035762252545080307	0.0
28	0.0	0.0	0.0	0.009669053465892082	0.0
29	0.0	0.0	0.0	0.020000370867804172	0.0
30	0.0	0.0	0.0	0.034702630247448296	0.0
31	0.0	0.0	0.0	0.07470337198305664	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	480	0.0	29.291666	1
CGTCTTC	165	0.0	26.90909	37
TTTACCG	35	8.866563E-4	26.428572	30
GTTACGG	35	8.866563E-4	26.428572	17
CTTATAC	620	0.0	25.362902	1
GTATCAA	1445	0.0	23.813148	1
CCGTCTT	415	0.0	23.626503	37
GCCGTCT	420	0.0	23.345238	36
TCGAGGT	40	0.0019305766	23.125	15
CGCCGTT	40	0.0019305766	23.125	25
TTACCGG	40	0.0019305766	23.125	31
GATTACG	40	0.0019305766	23.125	1
CCGTTTA	40	0.0019305766	23.125	27
TACCGGG	40	0.0019305766	23.125	32
TATACAC	705	0.0	22.829786	3
TTATACA	695	0.0	22.093523	2
GTCGCCC	95	7.1358954E-9	21.421053	37
GCTCGGA	95	7.1358954E-9	21.421053	11
GGACCGT	70	5.0980943E-6	21.142859	6
CCGCCGT	80	6.9536145E-7	20.8125	24
>>END_MODULE