FastQCFastQC Report
Fri 10 Feb 2017
ERR1630931.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630931.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123940
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT257652.2923821556310835No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG216231.9238571453992204No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT162861.4490097336156735TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC111370.9908891933733118No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC63480.564798832677901RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC52420.46639500329199063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28950.2575760271900635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28120.25019129135007206No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG26950.23978148299731306No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA20650.18372866879014896No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT20100.17883516913714254No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT17320.15410075270921936No Hit
GGGATATACAAAGTGGAAATAGACACCAAATCTTACTGGAAGG13240.11779988255600835No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT13230.11771090983504458No Hit
GTATATCCCTTCTACAAATTCCTCCTCAGTTGTGAGCCCATGC11820.10516575617915548No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11690.10400911080662668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC29050.034.516351
CGTCTTC7650.031.43790837
TTATACA33000.030.4969712
TATACAC34500.029.5463753
GGTATCA6650.026.9849621
CCGTCTT24800.025.43749837
GCCGTCT25250.024.9841636
TTACCGG551.9026105E-523.54545431
GTATCAA17900.022.8407821
TAGGACC903.8289727E-922.611114
TTACGGG1107.4578566E-1121.8636363
GATTACG1059.822543E-1021.1428571
TGCCGTC30250.020.915735
CGTTCGT450.00382575620.55555524
CGTCAAA450.00382575620.55555527
ATACCGA450.00382575620.5555556
ATGCCGT31600.019.96360834
GACCGTT759.265472E-619.7333347
AGTCGGT759.265472E-619.73333431
TAGTCGG851.2452583E-619.58823630
Produced by FastQC (version 0.11.5)