##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630931.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1123940
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.232423438973612	31.0	31.0	34.0	28.0	34.0
2	31.226968521451322	31.0	31.0	34.0	28.0	34.0
3	30.661802231435843	31.0	31.0	34.0	26.0	34.0
4	34.86109044966813	37.0	35.0	37.0	32.0	37.0
5	35.04096837909497	35.0	35.0	37.0	32.0	37.0
6	35.24698471448654	37.0	35.0	37.0	32.0	37.0
7	35.25800843461395	37.0	35.0	37.0	32.0	37.0
8	35.28341993344841	37.0	35.0	37.0	32.0	37.0
9	37.04367759844832	39.0	37.0	39.0	33.0	39.0
10	36.83716835418261	39.0	37.0	39.0	33.0	39.0
11	36.90907343808389	39.0	37.0	39.0	33.0	39.0
12	36.83245991778921	39.0	37.0	39.0	33.0	39.0
13	36.92029289819741	39.0	37.0	39.0	33.0	39.0
14	37.969081979465095	40.0	37.0	41.0	33.0	41.0
15	37.97178408100076	40.0	37.0	41.0	33.0	41.0
16	37.97047173336655	40.0	37.0	41.0	33.0	41.0
17	37.918725198854034	40.0	37.0	41.0	33.0	41.0
18	37.96345178568251	40.0	37.0	41.0	33.0	41.0
19	37.9661467694005	40.0	37.0	41.0	33.0	41.0
20	38.00142267380821	40.0	37.0	41.0	33.0	41.0
21	37.96552040144492	40.0	37.0	41.0	33.0	41.0
22	37.863935797284554	40.0	37.0	41.0	33.0	41.0
23	37.88477053935264	40.0	37.0	41.0	33.0	41.0
24	37.78780717831913	39.0	37.0	41.0	33.0	41.0
25	37.76198640496823	39.0	37.0	41.0	32.0	41.0
26	37.60351175329644	39.0	37.0	41.0	32.0	41.0
27	37.528738188871294	39.0	37.0	41.0	32.0	41.0
28	37.54819296403723	39.0	37.0	41.0	32.0	41.0
29	37.447781020339164	39.0	37.0	41.0	32.0	41.0
30	37.42246739149777	39.0	37.0	40.0	32.0	41.0
31	37.40832072886453	39.0	36.0	40.0	32.0	41.0
32	37.2987356976351	39.0	36.0	40.0	31.0	41.0
33	37.258778938377496	39.0	36.0	40.0	31.0	41.0
34	37.15878694592238	39.0	36.0	40.0	31.0	41.0
35	37.138751178888555	39.0	36.0	40.0	31.0	41.0
36	37.12827464099507	39.0	36.0	40.0	31.0	41.0
37	37.04884246490026	39.0	36.0	40.0	31.0	41.0
38	37.016652134455576	39.0	36.0	40.0	31.0	41.0
39	36.959096570991335	39.0	36.0	40.0	31.0	41.0
40	36.85968823958574	39.0	36.0	40.0	30.0	41.0
41	36.8247041657028	39.0	36.0	40.0	30.0	41.0
42	36.770486858729114	39.0	35.0	40.0	30.0	41.0
43	36.01356922967418	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	15.0
10	28.0
11	28.0
12	29.0
13	18.0
14	25.0
15	9.0
16	15.0
17	31.0
18	41.0
19	80.0
20	150.0
21	289.0
22	580.0
23	1054.0
24	1996.0
25	3323.0
26	5246.0
27	7719.0
28	11365.0
29	16192.0
30	22002.0
31	28285.0
32	36918.0
33	47525.0
34	62545.0
35	83635.0
36	115592.0
37	171752.0
38	284263.0
39	223188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.33375447087923	22.534032065768635	15.202590885634464	24.929622577717673
2	13.792462231079952	22.03124721960247	41.189654252006335	22.986636297311243
3	14.829172375749595	28.80029183052476	34.38982507963059	21.980710714095057
4	11.719575778066444	20.0397708062708	36.86273288609712	31.377920529565635
5	12.20198587113191	38.40970158549389	35.89702297275655	13.49128957061765
6	26.951972525223766	40.1313237361425	17.855757424773564	15.060946313860171
7	24.083669946794313	33.34920013523854	22.42539637347189	20.141733544495256
8	24.495791590298417	35.12634126376853	22.85308824314465	17.524778902788405
9	23.696816556043917	15.00071178176771	19.02743918714522	42.27503247504315
10	14.173532394967703	28.958040464793495	33.604551844404504	23.263875295834296
11	30.351709165969716	23.128636759969393	25.329643931170704	21.190010142890188
12	19.883178817374592	26.49251739416695	28.93134864850437	24.69295513995409
13	29.12833425271812	20.522180899336266	27.070573162268445	23.278911685677173
14	21.680961617168176	22.8352936989519	26.04943324376746	29.43431144011246
15	24.071391711301317	28.54298272149759	22.335355979856576	25.05026958734452
16	25.00498247237397	27.40662312934854	23.122586614943856	24.46580778333363
17	20.413990070644342	31.389753901453815	24.654252006334858	23.54200402156699
18	23.781429613680448	26.268128191896366	25.36692350125452	24.583518693168674
19	23.35854227093973	28.588091891026213	26.08039575066285	21.972970087371213
20	22.278502411160737	25.571204868587287	26.860775486235923	25.289517234016053
21	23.992028044201646	25.793458725554746	25.212822748545292	25.00169048169831
22	20.454650604124776	30.582326458707758	27.106073277932985	21.856949659234477
23	22.460273680089685	25.836165631617348	25.815346014911828	25.888214673381142
24	23.3900386141609	29.892165062191932	25.816680605726283	20.901115717920884
25	23.12712422371301	27.546666192145487	26.3026496076303	23.0235599765112
26	24.641528907237042	28.522430022954964	25.242183746463336	21.59385732334466
27	21.442425752264356	29.17584568571276	28.17374592949802	21.207982632524867
28	20.85342633948431	25.783582753527767	26.853746641279784	26.509244265708134
29	21.07114258768262	29.093723864263215	27.685107745965087	22.15002580208908
30	21.533622791252203	25.510347527448086	29.726675801199352	23.229353880100362
31	22.72167553428119	26.931775717564992	25.530277416943964	24.81627133120985
32	23.628307560901828	26.228624303788457	28.95252415609374	21.190543979215974
33	22.697119063295194	25.605637311600265	28.091268217164618	23.605975407939926
34	21.565030161752407	30.39165791768244	26.038133708205063	22.00517821236009
35	23.891221951349717	25.44441874121394	30.29779169706568	20.366567610370662
36	25.252148691211275	25.56150684200224	25.353221702226097	23.833122764560386
37	22.36195882342474	27.150737583856788	27.79098528391195	22.69631830880652
38	22.25439080377956	24.738954036692352	28.70891684609499	24.2977383134331
39	24.400946669751054	25.575030695588737	26.254871256472768	23.76915137818745
40	20.66631670729754	25.123761054860577	32.10029005107034	22.109632186771535
41	22.330284534761642	26.082264177803083	26.20584728722174	25.381604000213535
42	23.325889282346033	24.618395999786465	29.74874103599836	22.306973681869138
43	23.007011050411943	24.119792871505595	29.850525828780896	23.022670249301562
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14.0
1	21.0
2	28.0
3	109.5
4	191.0
5	191.0
6	269.5
7	348.0
8	343.5
9	339.0
10	523.5
11	708.0
12	708.0
13	1106.5
14	1505.0
15	3280.0
16	5055.0
17	5845.0
18	6635.0
19	6635.0
20	7206.0
21	7777.0
22	9673.0
23	11569.0
24	15064.0
25	18559.0
26	18559.0
27	22784.5
28	27010.0
29	32861.0
30	38712.0
31	44714.0
32	50716.0
33	50716.0
34	55049.0
35	59382.0
36	62244.5
37	65107.0
38	69051.5
39	72996.0
40	72996.0
41	73380.5
42	73765.0
43	73660.0
44	73555.0
45	74348.0
46	75141.0
47	75141.0
48	76986.0
49	78831.0
50	100642.0
51	122453.0
52	99247.5
53	76042.0
54	76042.0
55	83468.0
56	90894.0
57	77158.5
58	63423.0
59	48203.0
60	32983.0
61	32983.0
62	29315.5
63	25648.0
64	21391.5
65	17135.0
66	13997.5
67	10860.0
68	10860.0
69	8675.0
70	6490.0
71	5594.0
72	4698.0
73	3751.0
74	2804.0
75	2804.0
76	2085.0
77	1366.0
78	1051.5
79	737.0
80	497.0
81	257.0
82	257.0
83	182.5
84	108.0
85	87.0
86	66.0
87	44.5
88	23.0
89	23.0
90	16.0
91	9.0
92	5.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1123940.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.82511914041375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62366148072883	39.17433300532873
2	12.795560472497748	12.75080650021138
3	3.755093862516555	5.6129399724997135
4	1.4876967405716053	2.9649866937514173
5	0.7933849305251274	1.9765249343811675
6	0.46077927651377365	1.3775029409839454
7	0.3185840638948854	1.1111442257859898
8	0.2310016309213828	0.9207747025830225
9	0.1675353392238944	0.7512721413354154
>10	1.1549047978064446	11.376095990140055
>50	0.1290144903964602	4.452355842437634
>100	0.07546804519336339	6.698674988875476
>500	0.00410346321528748	1.26140181023572
>1k	0.002140937329715207	1.8906804159199104
>5k	3.568228882858678E-4	1.0302781522644113
>10k+	7.136457765717356E-4	6.650227683265994
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	25765	2.2923821556310835	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	21623	1.9238571453992204	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	16286	1.4490097336156735	TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	11137	0.9908891933733118	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	6348	0.564798832677901	RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	5242	0.46639500329199063	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2895	0.2575760271900635	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2812	0.25019129135007206	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	2695	0.23978148299731306	No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	2065	0.18372866879014896	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	2010	0.17883516913714254	No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT	1732	0.15410075270921936	No Hit
GGGATATACAAAGTGGAAATAGACACCAAATCTTACTGGAAGG	1324	0.11779988255600835	No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT	1323	0.11771090983504458	No Hit
GTATATCCCTTCTACAAATTCCTCCTCAGTTGTGAGCCCATGC	1182	0.10516575617915548	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1169	0.10400911080662668	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.897272096375251E-5	0.0
3	0.0	0.0	0.0	8.897272096375251E-5	0.0
4	0.0	0.0	0.0	8.897272096375251E-5	0.0
5	0.0	0.0	0.0	8.897272096375251E-5	0.0
6	0.0	0.0	0.0	8.897272096375251E-5	0.0
7	0.0	0.0	0.0	8.897272096375251E-5	0.0
8	0.0	0.0	0.0	8.897272096375251E-5	0.0
9	0.0	0.0	0.0	8.897272096375251E-5	0.0
10	0.0	0.0	0.0	8.897272096375251E-5	0.0
11	0.0	0.0	0.0	1.7794544192750503E-4	0.0
12	0.0	0.0	0.0	1.7794544192750503E-4	0.0
13	0.0	0.0	0.0	1.7794544192750503E-4	0.0
14	0.0	0.0	0.0	1.7794544192750503E-4	0.0
15	0.0	0.0	0.0	2.6691816289125755E-4	0.0
16	0.0	0.0	0.0	3.5589088385501005E-4	0.0
17	0.0	0.0	0.0	4.4486360481876255E-4	0.0
18	0.0	0.0	0.0	5.338363257825151E-4	0.0
19	0.0	0.0	0.0	5.338363257825151E-4	0.0
20	0.0	0.0	0.0	5.338363257825151E-4	0.0
21	0.0	0.0	0.0	5.338363257825151E-4	0.0
22	0.0	0.0	0.0	0.0010676726515650302	0.0
23	0.0	0.0	0.0	0.001245618093492535	0.0
24	0.0	0.0	0.0	0.0018684271402388028	0.0
25	0.0	0.0	0.0	0.0020463725821663076	0.0
26	0.0	0.0	0.0	0.00249123618698507	0.0
27	0.0	0.0	0.0	0.003114045233731338	0.0
28	0.0	0.0	0.0	0.009342135701194014	0.0
29	0.0	0.0	0.0	0.023577771055394416	0.0
30	0.0	0.0	0.0	0.04350766055127498	0.0
31	8.897272096375251E-5	0.0	0.0	0.09181984803459259	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2905	0.0	34.51635	1
CGTCTTC	765	0.0	31.437908	37
TTATACA	3300	0.0	30.496971	2
TATACAC	3450	0.0	29.546375	3
GGTATCA	665	0.0	26.984962	1
CCGTCTT	2480	0.0	25.437498	37
GCCGTCT	2525	0.0	24.98416	36
TTACCGG	55	1.9026105E-5	23.545454	31
GTATCAA	1790	0.0	22.840782	1
TAGGACC	90	3.8289727E-9	22.61111	4
TTACGGG	110	7.4578566E-11	21.863636	3
GATTACG	105	9.822543E-10	21.142857	1
TGCCGTC	3025	0.0	20.9157	35
CGTTCGT	45	0.003825756	20.555555	24
CGTCAAA	45	0.003825756	20.555555	27
ATACCGA	45	0.003825756	20.555555	6
ATGCCGT	3160	0.0	19.963608	34
GACCGTT	75	9.265472E-6	19.733334	7
AGTCGGT	75	9.265472E-6	19.733334	31
TAGTCGG	85	1.2452583E-6	19.588236	30
>>END_MODULE