FastQCFastQC Report
Fri 10 Feb 2017
ERR1630930.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630930.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153421
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG60540.5248733983515126No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT50310.43618071805524605No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT41930.3635272810188127RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC33200.28783939255484337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32940.2855852286372452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31920.27674197019128316No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC17540.15206936582566122RNA PCR Primer, Index 29 (95% over 21bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC14580.1264065766099282RNA PCR Primer, Index 29 (95% over 24bp)
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT12420.10767967637142033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG800.037.01
TTACGGG950.031.1578963
CTTATAC9350.026.117651
GGTATCA5800.024.879311
TATACAC10550.024.7251173
CCGTCTT6500.023.90769437
GCCGTCT6700.023.4701536
GTATCAA18500.023.41
GTCGTAC400.001931190723.1250023
TTATACA11100.022.8333322
CGTCTTC2800.022.46428537
TCACGCA450.003825816320.5555554
ATTACGG1351.8189894E-1220.5555552
GCGGGTA759.265816E-619.73333422
GCAACGC851.2453165E-619.5882343
TGCCGTC8350.018.61077935
TACGTAC500.00703489618.510
ATGCCGT8850.017.55932234
TATCGCG1401.869921E-917.1785727
CGAACCG2750.016.81818231
Produced by FastQC (version 0.11.5)