##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630930.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1153421
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.74528901415875	31.0	30.0	33.0	27.0	34.0
2	30.71006770294628	31.0	30.0	34.0	27.0	34.0
3	30.102471690735648	31.0	30.0	34.0	25.0	34.0
4	34.40901717586207	35.0	35.0	37.0	30.0	37.0
5	34.436248342972775	35.0	35.0	37.0	30.0	37.0
6	34.675930991372624	35.0	35.0	37.0	32.0	37.0
7	34.721432157035466	35.0	35.0	37.0	32.0	37.0
8	34.7566872807067	35.0	35.0	37.0	32.0	37.0
9	36.419345581535275	38.0	35.0	39.0	32.0	39.0
10	36.14786535011934	37.0	35.0	39.0	32.0	39.0
11	36.2745588991357	38.0	35.0	39.0	32.0	39.0
12	36.18453366116969	37.0	35.0	39.0	32.0	39.0
13	36.298329057646775	38.0	35.0	39.0	32.0	39.0
14	37.25493640223301	39.0	36.0	40.0	32.0	41.0
15	37.261287942563904	39.0	36.0	40.0	32.0	41.0
16	37.2627982323887	39.0	36.0	40.0	32.0	41.0
17	37.20731718947375	39.0	36.0	40.0	32.0	41.0
18	37.261396315829174	39.0	36.0	40.0	32.0	41.0
19	37.28315940146746	39.0	36.0	40.0	32.0	41.0
20	37.282785730448815	39.0	36.0	40.0	32.0	41.0
21	37.224224285841856	39.0	36.0	40.0	32.0	41.0
22	37.1336771222303	39.0	36.0	40.0	31.0	41.0
23	37.126368429220555	39.0	36.0	40.0	31.0	41.0
24	37.08020228520202	39.0	36.0	40.0	31.0	41.0
25	37.04120698339982	39.0	36.0	40.0	31.0	41.0
26	36.89743380777704	39.0	36.0	40.0	31.0	41.0
27	36.81729221160357	39.0	36.0	40.0	31.0	41.0
28	36.77003193109888	39.0	36.0	40.0	31.0	41.0
29	36.687203544932856	38.0	36.0	40.0	30.0	41.0
30	36.60599468884302	38.0	35.0	40.0	30.0	41.0
31	36.580592862450054	38.0	35.0	40.0	30.0	41.0
32	36.50027180014929	38.0	35.0	40.0	30.0	41.0
33	36.46682520952887	38.0	35.0	40.0	30.0	41.0
34	36.36209241898665	38.0	35.0	40.0	30.0	41.0
35	36.31675858164538	38.0	35.0	40.0	30.0	41.0
36	36.289039301347906	38.0	35.0	40.0	30.0	41.0
37	36.22717463961554	38.0	35.0	40.0	30.0	41.0
38	36.184537996100296	38.0	35.0	40.0	30.0	41.0
39	36.11095428295479	38.0	35.0	40.0	30.0	41.0
40	36.04960461097899	38.0	35.0	40.0	30.0	41.0
41	35.974207162865945	38.0	35.0	40.0	29.0	41.0
42	35.92383700314109	38.0	35.0	40.0	29.0	41.0
43	35.17883756234714	38.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	8.0
11	9.0
12	6.0
13	11.0
14	5.0
15	6.0
16	15.0
17	27.0
18	63.0
19	110.0
20	247.0
21	484.0
22	963.0
23	1595.0
24	2884.0
25	4491.0
26	7251.0
27	10548.0
28	15307.0
29	21321.0
30	28659.0
31	38026.0
32	49340.0
33	63885.0
34	83953.0
35	112225.0
36	155398.0
37	212309.0
38	247489.0
39	96780.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.98212361314732	22.93707154629576	15.583728751253878	22.49707608930304
2	15.269186186136718	23.792526753024266	38.92949755553263	22.00878950530639
3	16.540534635662087	28.059138857364307	33.7033919098057	21.696934597167903
4	12.982510288957805	19.2183946711565	39.59204835008206	28.20704668980364
5	12.522747548379993	39.09379142568065	35.727284313359995	12.656176712579361
6	27.326362186920473	41.27946343962872	18.019959754504207	13.374214618946596
7	24.57636890606292	33.80569627221977	23.885207569482436	17.732727252234874
8	24.57836297414387	36.02500734770738	21.832878021121516	17.563751657027225
9	25.011075747710503	15.6636648717164	20.866708686594055	38.45855069397904
10	14.677468157767198	29.067617114652844	34.49243597957727	21.762478748002682
11	31.844573663909358	24.14842455616813	25.45878738119039	18.548214398732117
12	20.837057761216414	26.751550387932944	30.937099289851666	21.474292560998975
13	27.81256800422396	22.195538316018176	27.452335270469323	22.539558409288542
14	21.26734297364102	22.383067414239903	28.426740973157244	27.922848638961835
15	23.09278225383446	29.553476137507467	24.48039354234057	22.873348066317504
16	22.483117612736372	29.092933109419718	25.23458477000159	23.18936450784232
17	21.422706886730865	29.356410191942057	26.898938028698975	22.321944892628103
18	22.64212286753926	27.649314517422518	27.770085684238456	21.93847693079977
19	23.88754843201225	27.401963376772226	28.163003794798257	20.547484396417268
20	23.801976901755733	26.7903913662054	27.524035022771393	21.883596709267476
21	22.915310194629715	27.431267507701	27.24798664147783	22.405435656191454
22	21.824728351573278	29.117382118064437	27.54622986749851	21.511659662863778
23	22.625129939545058	27.16302200150682	27.766010849464333	22.445837209483788
24	22.26905873917676	27.84820113384445	27.636829917263515	22.245910209715273
25	22.949122653393687	27.513717887917764	27.799736609616087	21.737422849072455
26	22.37327047105957	27.85548381727054	27.51475827126435	22.256487440405543
27	22.442976155280682	28.435931026052064	27.376907477842	21.74418534082525
28	21.882296230084243	27.588885584708443	27.959175357480053	22.569642827727257
29	22.11794305808547	28.19976400637755	27.797222349861844	21.885070585675134
30	22.836241060289346	27.80779958055211	27.858604967310292	21.49735439184825
31	22.658595603860167	27.438550191127092	27.107968382750098	22.794885822262643
32	21.84700989491261	28.052809858672596	28.31004464111543	21.790135605299366
33	22.15678403635793	27.776414682930174	28.34593786657257	21.72086341413933
34	22.010870271999554	27.775721094032445	27.47574389576746	22.737664738200536
35	21.9494009559389	27.610820333598916	29.23390505288182	21.205873657580362
36	22.582127427886263	27.87386392306018	26.9239939276292	22.620014721424354
37	22.855141357752288	26.837815507087175	27.97339392988337	22.33364920527717
38	22.676802312425384	26.830966316722165	27.852102571394138	22.640128799458306
39	22.77693920953407	27.10779498552567	27.869442293837203	22.245823511103058
40	21.98694145502813	26.80911826644391	29.072385538324685	22.131554740203274
41	22.284404393538875	26.894169605027134	27.79938981516723	23.022036186266767
42	22.348387969353777	26.805563623343083	28.35261365971315	22.493434747589998
43	22.503231690770324	26.313115505960095	28.595022979467167	22.58862982380241
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	15.5
2	18.0
3	95.5
4	173.0
5	173.0
6	237.5
7	302.0
8	308.5
9	315.0
10	480.5
11	646.0
12	646.0
13	1072.5
14	1499.0
15	3628.5
16	5758.0
17	6691.0
18	7624.0
19	7624.0
20	8596.5
21	9569.0
22	12592.5
23	15616.0
24	19989.0
25	24362.0
26	24362.0
27	29067.5
28	33773.0
29	39455.0
30	45137.0
31	50832.0
32	56527.0
33	56527.0
34	61054.0
35	65581.0
36	70286.0
37	74991.0
38	78995.0
39	82999.0
40	82999.0
41	86040.5
42	89082.0
43	87098.5
44	85115.0
45	85270.5
46	85426.0
47	85426.0
48	84647.0
49	83868.0
50	86882.5
51	89897.0
52	83287.5
53	76678.0
54	76678.0
55	72201.5
56	67725.0
57	59012.5
58	50300.0
59	41542.5
60	32785.0
61	32785.0
62	28517.5
63	24250.0
64	20380.0
65	16510.0
66	13758.5
67	11007.0
68	11007.0
69	8836.0
70	6665.0
71	5520.0
72	4375.0
73	3369.0
74	2363.0
75	2363.0
76	1809.0
77	1255.0
78	987.0
79	719.0
80	496.0
81	273.0
82	273.0
83	200.0
84	127.0
85	91.5
86	56.0
87	40.5
88	25.0
89	25.0
90	20.0
91	15.0
92	8.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1153421.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.70052812390788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.77582299082528	38.65499477915592
2	12.795714824490753	12.719075690002152
3	3.990901910595654	5.95049797941951
4	1.6455921940322835	3.271472044799391
5	0.8909069633430834	2.2139273293709145
6	0.5160933915449912	1.5390068472626903
7	0.35565937308653833	1.237352107223326
8	0.2570087906615876	1.021877810269423
9	0.18025510745634507	0.8062896633850902
>10	1.3193427292550748	12.781296714341723
>50	0.1611810842382897	5.657157700125867
>100	0.10345468309843416	9.43363363380327
>500	0.006137141478510107	1.947416694887434
>1k	0.0015781220944740277	1.7999617119934246
>5k	3.506937987720061E-4	0.9660392939598679
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	6054	0.5248733983515126	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	5031	0.43618071805524605	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	4193	0.3635272810188127	RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	3320	0.28783939255484337	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3294	0.2855852286372452	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3192	0.27674197019128316	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	1754	0.15206936582566122	RNA PCR Primer, Index 29 (95% over 21bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC	1458	0.1264065766099282	RNA PCR Primer, Index 29 (95% over 24bp)
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT	1242	0.10767967637142033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.669861221531427E-5	0.0
10	0.0	0.0	0.0	8.669861221531427E-5	0.0
11	0.0	0.0	0.0	8.669861221531427E-5	0.0
12	0.0	0.0	0.0	8.669861221531427E-5	0.0
13	0.0	0.0	0.0	8.669861221531427E-5	0.0
14	0.0	0.0	0.0	8.669861221531427E-5	0.0
15	0.0	0.0	0.0	8.669861221531427E-5	0.0
16	0.0	0.0	0.0	8.669861221531427E-5	0.0
17	0.0	0.0	0.0	8.669861221531427E-5	0.0
18	0.0	0.0	0.0	8.669861221531427E-5	0.0
19	0.0	0.0	0.0	8.669861221531427E-5	0.0
20	0.0	0.0	0.0	1.7339722443062853E-4	0.0
21	0.0	0.0	0.0	2.600958366459428E-4	0.0
22	0.0	0.0	0.0	6.068902855071999E-4	0.0
23	0.0	0.0	0.0	7.802875099378285E-4	0.0
24	0.0	0.0	0.0	0.0010403833465837712	0.0
25	0.0	0.0	0.0	0.001300479183229714	0.0
26	0.0	0.0	0.0	0.001647273632090971	0.0
27	0.0	0.0	0.0	0.002254163917598171	0.0
28	0.0	0.0	0.0	0.007889573711593599	0.0
29	0.0	0.0	0.0	0.01907369468736914	0.0
30	0.0	0.0	0.0	0.035199636559417595	0.0
31	0.0	0.0	0.0	0.08409765384885484	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	80	0.0	37.0	1
TTACGGG	95	0.0	31.157896	3
CTTATAC	935	0.0	26.11765	1
GGTATCA	580	0.0	24.87931	1
TATACAC	1055	0.0	24.725117	3
CCGTCTT	650	0.0	23.907694	37
GCCGTCT	670	0.0	23.47015	36
GTATCAA	1850	0.0	23.4	1
GTCGTAC	40	0.0019311907	23.125002	3
TTATACA	1110	0.0	22.833332	2
CGTCTTC	280	0.0	22.464285	37
TCACGCA	45	0.0038258163	20.555555	4
ATTACGG	135	1.8189894E-12	20.555555	2
GCGGGTA	75	9.265816E-6	19.733334	22
GCAACGC	85	1.2453165E-6	19.588234	3
TGCCGTC	835	0.0	18.610779	35
TACGTAC	50	0.007034896	18.5	10
ATGCCGT	885	0.0	17.559322	34
TATCGCG	140	1.869921E-9	17.178572	7
CGAACCG	275	0.0	16.818182	31
>>END_MODULE