FastQCFastQC Report
Fri 10 Feb 2017
ERR1630929.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630929.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799578
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT151931.9001273171598019No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT141001.7634302094354772TruSeq Adapter, Index 8 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG140591.7583025045711613No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC76080.9515019172613554No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC56440.7058723476633925RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC38540.48200425724569707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20610.25776096891110056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20370.2547593855758913No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG18650.23324803834022445No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA13250.16571241329801473No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT12200.1525804862064739No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC11600.14507652786845063No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA11100.13882322925343119No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT10800.13507125008441953No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT10070.12594143410649117No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC9750.12193932299287874No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT9590.11993826743607254No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG9020.11280950701495038No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT9010.11268444104264999No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC8960.11205911118114806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8440.10555568062152786No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA8040.10055304172951231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19800.032.3282851
CGTCTTC7950.030.94968437
TTATACA21150.030.6146552
TATACAC22150.029.399553
TCGCCGA358.867024E-426.4285721
CGGTGTA358.867024E-426.42857234
TTACGGG509.086567E-625.9000023
GGTATCA3250.024.4769231
GTATCAA10350.024.1304361
CCGTCTT22350.024.00447337
GCCGTCT22800.023.61184336
GATTACG551.9017205E-523.5454541
CGAACTA652.6811504E-622.7692329
GTCGCCA1800.022.6111112
TCGCCAT1750.022.213
ATGCCGG502.701105E-422.234
ATTACGG603.724461E-521.5833322
CAGTGCG450.003824804720.5555559
TAGTCGG450.003824804720.55555530
GTAGTCG450.003824804720.55555529
Produced by FastQC (version 0.11.5)