##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630929.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	799578
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.212689693813488	31.0	31.0	34.0	28.0	34.0
2	31.22213842802078	31.0	31.0	34.0	28.0	34.0
3	30.67115278309308	31.0	31.0	34.0	26.0	34.0
4	34.8567744485216	37.0	35.0	37.0	32.0	37.0
5	35.02214167973606	35.0	35.0	37.0	32.0	37.0
6	35.225850386078655	37.0	35.0	37.0	32.0	37.0
7	35.233905885354524	37.0	35.0	37.0	32.0	37.0
8	35.274340964858965	37.0	35.0	37.0	32.0	37.0
9	37.00855576316507	39.0	37.0	39.0	33.0	39.0
10	36.817678825580494	39.0	37.0	39.0	33.0	39.0
11	36.88433648749715	39.0	37.0	39.0	33.0	39.0
12	36.80961707300601	39.0	37.0	39.0	33.0	39.0
13	36.89432925868395	39.0	37.0	39.0	33.0	39.0
14	37.94645550527903	40.0	37.0	41.0	33.0	41.0
15	37.969296303800256	40.0	37.0	41.0	33.0	41.0
16	37.95095662962213	40.0	37.0	41.0	33.0	41.0
17	37.897098219310685	40.0	37.0	41.0	33.0	41.0
18	37.96556308452709	40.0	37.0	41.0	33.0	41.0
19	37.98473194610157	40.0	37.0	41.0	33.0	41.0
20	37.985097138740684	40.0	37.0	41.0	33.0	41.0
21	37.950940371045725	40.0	37.0	41.0	33.0	41.0
22	37.880036719369464	40.0	37.0	41.0	33.0	41.0
23	37.86596929880512	40.0	37.0	41.0	33.0	41.0
24	37.82338808721601	40.0	37.0	41.0	33.0	41.0
25	37.77728751916636	39.0	37.0	41.0	32.0	41.0
26	37.63929722928845	39.0	37.0	41.0	32.0	41.0
27	37.57668795289515	39.0	37.0	41.0	32.0	41.0
28	37.56462784118622	39.0	37.0	41.0	32.0	41.0
29	37.518497257303224	39.0	37.0	41.0	32.0	41.0
30	37.434495446347945	39.0	37.0	40.0	32.0	41.0
31	37.40114160219516	39.0	36.0	40.0	32.0	41.0
32	37.30821008081763	39.0	36.0	40.0	31.0	41.0
33	37.23350317292372	39.0	36.0	40.0	31.0	41.0
34	37.1776612162916	39.0	36.0	40.0	31.0	41.0
35	37.17521617653312	39.0	36.0	40.0	31.0	41.0
36	37.131676959596184	39.0	36.0	40.0	31.0	41.0
37	37.093039078113705	39.0	36.0	40.0	31.0	41.0
38	37.04668212482084	39.0	36.0	40.0	31.0	41.0
39	36.9313012614154	39.0	36.0	40.0	31.0	41.0
40	36.8285470585734	39.0	36.0	40.0	30.0	41.0
41	36.79815852862385	39.0	36.0	40.0	30.0	41.0
42	36.760557444051734	39.0	35.0	40.0	30.0	41.0
43	36.023496144216075	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	11.0
10	18.0
11	19.0
12	16.0
13	8.0
14	14.0
15	17.0
16	12.0
17	23.0
18	34.0
19	57.0
20	93.0
21	223.0
22	393.0
23	755.0
24	1405.0
25	2295.0
26	3686.0
27	5640.0
28	8114.0
29	11383.0
30	15536.0
31	20245.0
32	26274.0
33	33615.0
34	44604.0
35	59514.0
36	82741.0
37	122331.0
38	202974.0
39	157520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.72799901948277	23.198737333943654	15.880376898814125	24.19288674775944
2	13.536765643877144	22.297762069491654	41.2757729702418	22.889699316389393
3	14.608831158436075	28.591832191481004	35.63529761949428	21.164039030588636
4	11.620629882262893	19.633731793521083	37.191118314911115	31.55452000930491
5	11.97381618803919	39.01045301396487	35.74022797025431	13.275502827741633
6	27.02463049258484	40.33727791409969	17.9005675493823	14.737524043933176
7	23.150712000580306	34.59349807023205	22.754753132277276	19.50103679691037
8	24.24591472001481	35.791129820980565	22.550770531455342	17.412184927549283
9	22.940851299060256	15.390243353368902	19.412114890604794	42.25679045696605
10	13.746876477341798	29.061204785524364	34.05671491711878	23.135203820015057
11	29.989319365965546	23.263271375650657	25.76258976610162	20.984819492282178
12	19.14972648071858	27.495754010240404	29.095472861934667	24.25904664710635
13	28.336197344099013	20.76257725950439	27.450605194239962	23.45062020215664
14	21.3460100202857	23.449494608405935	26.30825260324821	28.896242768060155
15	23.71926191065787	29.485178431622682	22.960236524766813	23.83532313295263
16	24.640747994567132	28.29067333018167	23.338060827086288	23.730517848164904
17	20.223793050834317	31.867184940055882	24.712160664750655	23.19686134435915
18	23.22612678187744	26.166302724687274	25.91429479050199	24.6932757029333
19	24.62286356052818	27.719497034685798	25.994086880829638	21.663552523956387
20	21.71658049621175	25.809864703631163	26.861794596649734	25.61176020350735
21	23.368701990299883	26.19144098511965	27.067653187056173	23.372203837524292
22	19.71027216856892	31.60292054058516	25.68404833549697	23.002758955348945
23	22.0533831596167	27.735880677057146	27.146194617660818	23.06454154566534
24	24.398495206221284	27.734755083306446	27.296148718448983	20.57060099202329
25	22.745373184354747	28.537053295613436	25.890907453681816	22.82666606635
26	24.067820775459055	28.380345632321045	26.853290110533308	20.698543481686592
27	20.736688603238207	29.540832789296356	26.214828321939827	23.507650285525614
28	19.800694866542102	27.40070387129211	30.03271725835378	22.765884003812012
29	22.709104052387634	26.4639597387622	27.068778780806873	23.75815742804329
30	20.85124903386536	28.465265427513014	27.861697045191335	22.821788493430283
31	21.351512923066917	26.71834392642119	29.616997966427288	22.313145184084604
32	23.27327665343469	26.901315443896657	27.3982025518461	22.42720535082256
33	20.10973288409636	29.93866764718389	29.356235414180983	20.59536405453877
34	20.717428443503948	29.129490806400376	28.551936146317182	21.601144603778494
35	23.028147347725923	26.33376606159749	30.62778115455903	20.01030543611755
36	22.51037422240232	28.48977835808389	25.16665040809027	23.833197011423525
37	22.023492392236903	26.878803568882585	28.036164076550378	23.06153996233013
38	22.072518253378657	25.331487359582177	28.69851346585324	23.897480921185927
39	24.128352706052443	26.10502039826008	26.92432758279993	22.842299312887548
40	20.592987801065064	25.034330609396456	32.28615594726218	22.0865256422763
41	21.668555162848403	26.817521242455395	26.93145634322105	24.582467251475155
42	22.403442816085484	25.144638796965403	30.538734182281154	21.913184204667964
43	22.201461270820356	24.876372286381066	30.15753309870957	22.76463334408901
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	16.0
2	22.0
3	72.5
4	123.0
5	123.0
6	165.5
7	208.0
8	233.0
9	258.0
10	422.0
11	586.0
12	586.0
13	815.5
14	1045.0
15	2611.5
16	4178.0
17	4786.5
18	5395.0
19	5395.0
20	6186.0
21	6977.0
22	8793.5
23	10610.0
24	13214.5
25	15819.0
26	15819.0
27	19030.0
28	22241.0
29	27330.5
30	32420.0
31	34752.0
32	37084.0
33	37084.0
34	40080.5
35	43077.0
36	45179.5
37	47282.0
38	50851.5
39	54421.0
40	54421.0
41	56503.5
42	58586.0
43	55070.5
44	51555.0
45	52325.0
46	53095.0
47	53095.0
48	54414.5
49	55734.0
50	69158.0
51	82582.0
52	68076.5
53	53571.0
54	53571.0
55	57539.5
56	61508.0
57	50943.0
58	40378.0
59	30246.0
60	20114.0
61	20114.0
62	17563.5
63	15013.0
64	12498.0
65	9983.0
66	8127.0
67	6271.0
68	6271.0
69	5083.5
70	3896.0
71	3245.5
72	2595.0
73	2058.0
74	1521.0
75	1521.0
76	1140.5
77	760.0
78	569.0
79	378.0
80	262.5
81	147.0
82	147.0
83	115.5
84	84.0
85	57.0
86	30.0
87	22.5
88	15.0
89	15.0
90	9.5
91	4.0
92	2.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	799578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.68090293148771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.30918623487774	38.76895416303462
2	11.235176099414753	10.71406682028731
3	2.9493779705978147	4.218870141730278
4	1.216918127835544	2.320950205155773
5	0.6709761155112101	1.5996373516518347
6	0.39987571424596857	1.1439861069372808
7	0.3064105605878165	1.0226952537599288
8	0.22101793343356643	0.8430667704131114
9	0.16751652888995117	0.7188605418079366
>10	1.234982927010733	11.658804903872397
>50	0.1599322241171445	5.334736716832648
>100	0.11889685622084473	10.499767665276703
>500	0.005787015125776118	1.9592291787494645
>1k	0.0026304614208073267	2.0969433864357145
>5k	5.260922841614653E-4	1.6621026231859612
>10k+	7.891384262421979E-4	5.437328170869136
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	15193	1.9001273171598019	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	14100	1.7634302094354772	TruSeq Adapter, Index 8 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	14059	1.7583025045711613	No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	7608	0.9515019172613554	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	5644	0.7058723476633925	RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	3854	0.48200425724569707	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2061	0.25776096891110056	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2037	0.2547593855758913	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	1865	0.23324803834022445	No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	1325	0.16571241329801473	No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT	1220	0.1525804862064739	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1160	0.14507652786845063	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1110	0.13882322925343119	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1080	0.13507125008441953	No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT	1007	0.12594143410649117	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	975	0.12193932299287874	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	959	0.11993826743607254	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	902	0.11280950701495038	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	901	0.11268444104264999	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	896	0.11205911118114806	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	844	0.10555568062152786	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	804	0.10055304172951231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.2506597230038845E-4	0.0
11	0.0	0.0	0.0	1.2506597230038845E-4	0.0
12	0.0	0.0	0.0	1.2506597230038845E-4	0.0
13	0.0	0.0	0.0	1.2506597230038845E-4	0.0
14	0.0	0.0	0.0	1.2506597230038845E-4	0.0
15	0.0	0.0	0.0	1.2506597230038845E-4	0.0
16	0.0	0.0	0.0	1.2506597230038845E-4	0.0
17	0.0	0.0	0.0	1.2506597230038845E-4	0.0
18	0.0	0.0	0.0	1.2506597230038845E-4	0.0
19	0.0	1.2506597230038845E-4	0.0	1.2506597230038845E-4	0.0
20	0.0	1.2506597230038845E-4	0.0	1.2506597230038845E-4	0.0
21	0.0	1.2506597230038845E-4	0.0	3.7519791690116536E-4	0.0
22	0.0	1.2506597230038845E-4	0.0	6.253298615019423E-4	0.0
23	0.0	1.2506597230038845E-4	0.0	0.0010005277784031076	0.0
24	0.0	1.2506597230038845E-4	0.0	0.0011255937507034961	0.0
25	0.0	1.2506597230038845E-4	0.0	0.0011255937507034961	0.0
26	0.0	1.2506597230038845E-4	0.0	0.001375725695304273	0.0
27	0.0	1.2506597230038845E-4	0.0	0.002001055556806215	0.0
28	0.0	1.2506597230038845E-4	0.0	0.006128232642719034	0.0
29	0.0	1.2506597230038845E-4	0.0	0.01538311459294778	0.0
30	0.0	1.2506597230038845E-4	0.0	0.028014777795287014	0.0
31	0.0	1.2506597230038845E-4	0.0	0.06578470143000432	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1980	0.0	32.328285	1
CGTCTTC	795	0.0	30.949684	37
TTATACA	2115	0.0	30.614655	2
TATACAC	2215	0.0	29.39955	3
TCGCCGA	35	8.867024E-4	26.428572	1
CGGTGTA	35	8.867024E-4	26.428572	34
TTACGGG	50	9.086567E-6	25.900002	3
GGTATCA	325	0.0	24.476923	1
GTATCAA	1035	0.0	24.130436	1
CCGTCTT	2235	0.0	24.004473	37
GCCGTCT	2280	0.0	23.611843	36
GATTACG	55	1.9017205E-5	23.545454	1
CGAACTA	65	2.6811504E-6	22.76923	29
GTCGCCA	180	0.0	22.61111	12
TCGCCAT	175	0.0	22.2	13
ATGCCGG	50	2.701105E-4	22.2	34
ATTACGG	60	3.724461E-5	21.583332	2
CAGTGCG	45	0.0038248047	20.555555	9
TAGTCGG	45	0.0038248047	20.555555	30
GTAGTCG	45	0.0038248047	20.555555	29
>>END_MODULE