FastQCFastQC Report
Fri 10 Feb 2017
ERR1630928.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630928.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642265
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT114351.7804177403408252No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG92151.4347660233704156No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT70411.0962764590939877TruSeq Adapter, Index 6 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67751.054860532646182No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63970.9960063213782473No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC53820.8379718651958304No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30840.48017562843997413No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC27400.42661518220672157No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC26790.4171175449386157RNA PCR Primer, Index 6 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23200.36122161413123866No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA22110.34425042622593477No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16790.26141857333032315No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14800.23043447798027297No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14030.21844565716643444No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG13190.20536694355133786No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12980.2020972651475637No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG12620.19649210216966514No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12400.19306672479428275No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11700.18216779678170225No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11460.17843102146310325No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9250.1440215487376706No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA9200.14324305387962913No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8740.13608090118564767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8610.13405681455473986No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8540.13296692175348182No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8410.13094283512257401No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8100.12611616700271694No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8050.12533767214467548No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT7940.12362498345698428No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7930.12346928448537597No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7870.12253509065572622No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7740.12051100402481843No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7720.12019960608160184No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7670.11942111122356038No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7220.1124146575011872No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7100.1105462698418877No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7090.1103905708702794No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6950.1082107852677633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAA308.306273E-637.031
AGCAACG200.001841331837.02
ATTACTT501.8007995E-1037.02
CGAGAAC308.306273E-637.032
GATTACG1050.033.476191
CGTCTTC3750.032.06666637
TTATACA14600.031.9315072
AGAACGA352.38668E-531.71428534
AACGAGA352.38668E-531.71428536
ACGAGAG352.38668E-531.71428537
TATACAC15500.030.9129053
CTTATAC15300.030.5915031
AAGCAAG454.004638E-628.77777732
GCAAAGC405.936113E-527.7522
ACCAAGC405.936113E-527.7535
AGTTTAG556.2545223E-726.909099
CGACCCA358.865119E-426.4285722
GGTATCA7300.025.595891
GTATCAA24350.025.1478421
CCGTCTT12100.025.07437937
Produced by FastQC (version 0.11.5)