##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630928.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	642265
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.286699415350363	31.0	31.0	34.0	28.0	34.0
2	31.244699617759025	31.0	31.0	34.0	28.0	34.0
3	30.677162853339354	31.0	31.0	34.0	26.0	34.0
4	34.8940219379851	37.0	35.0	37.0	32.0	37.0
5	35.12058418254147	35.0	35.0	37.0	32.0	37.0
6	35.33687496594085	37.0	35.0	37.0	33.0	37.0
7	35.35485196920274	37.0	35.0	37.0	33.0	37.0
8	35.375804379812074	37.0	35.0	37.0	33.0	37.0
9	37.14070827462184	39.0	37.0	39.0	33.0	39.0
10	36.96378753318334	39.0	37.0	39.0	33.0	39.0
11	37.02338287155613	39.0	37.0	39.0	33.0	39.0
12	36.92859489463072	39.0	37.0	39.0	33.0	39.0
13	37.02118595906674	39.0	37.0	39.0	33.0	39.0
14	38.01514483896834	40.0	37.0	41.0	33.0	41.0
15	38.0270682662141	40.0	37.0	41.0	33.0	41.0
16	38.032450779662604	40.0	37.0	41.0	33.0	41.0
17	38.044945622134165	40.0	37.0	41.0	33.0	41.0
18	38.10438837551478	40.0	37.0	41.0	33.0	41.0
19	38.113773909523324	40.0	37.0	41.0	33.0	41.0
20	38.09771356060193	40.0	37.0	41.0	33.0	41.0
21	38.04225202992534	40.0	37.0	41.0	33.0	41.0
22	37.97227779810515	40.0	37.0	41.0	33.0	41.0
23	37.94591017726328	40.0	37.0	41.0	33.0	41.0
24	37.88773014254241	40.0	37.0	41.0	33.0	41.0
25	37.84344157006843	40.0	37.0	41.0	33.0	41.0
26	37.6624041478206	39.0	37.0	41.0	32.0	41.0
27	37.58244338396145	39.0	37.0	41.0	32.0	41.0
28	37.535573322538205	39.0	36.0	41.0	32.0	41.0
29	37.45149743485944	39.0	36.0	41.0	32.0	41.0
30	37.292695382747	39.0	36.0	41.0	32.0	41.0
31	37.14462877472694	39.0	36.0	40.0	31.0	41.0
32	37.03492483632146	39.0	35.0	40.0	31.0	41.0
33	36.94561279222751	39.0	35.0	40.0	31.0	41.0
34	36.875244642009136	39.0	35.0	40.0	31.0	41.0
35	36.80589009209594	39.0	35.0	40.0	31.0	41.0
36	36.6853868730197	39.0	35.0	40.0	30.0	41.0
37	36.62175426031311	39.0	35.0	40.0	30.0	41.0
38	36.538168824394916	39.0	35.0	40.0	30.0	41.0
39	36.413357414774275	39.0	35.0	40.0	30.0	41.0
40	36.25423462278032	39.0	35.0	40.0	30.0	41.0
41	36.11275408125929	38.0	35.0	40.0	29.0	41.0
42	35.946780534514566	38.0	35.0	40.0	29.0	41.0
43	35.182742325986936	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	12.0
11	12.0
12	4.0
13	11.0
14	4.0
15	8.0
16	8.0
17	13.0
18	27.0
19	53.0
20	101.0
21	209.0
22	417.0
23	715.0
24	1247.0
25	2188.0
26	3406.0
27	5200.0
28	7291.0
29	10215.0
30	13360.0
31	17120.0
32	21760.0
33	27905.0
34	36705.0
35	48432.0
36	66354.0
37	98280.0
38	151926.0
39	129273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.763960359041825	21.454695491736278	15.513222735163835	23.26812141405806
2	15.023549469455755	22.544432594022716	40.170178975967865	22.261838960553664
3	15.2572536258398	30.051925607031365	32.40936373615252	22.28145703097631
4	11.588363058861995	18.92116182572614	37.72508232583124	31.765392789580627
5	13.289685721625807	38.08381275641675	33.66024927405354	14.966252247903903
6	26.817435170840696	40.60582469852786	16.754299237853534	15.822440892777903
7	25.34716978194359	31.25874833596724	22.288307785727074	21.105774096362094
8	25.89896693732338	32.63730703058707	21.763290853463914	19.700435178625643
9	24.929273742146933	13.563404513713188	18.850474492615977	42.65684725152391
10	16.598288868302024	27.09893891150849	31.47672689621885	24.826045323970636
11	32.680747043665775	23.45900835324983	23.56457225600025	20.29567234708415
12	21.925684880851364	27.076673958568502	26.74846052641822	24.249180634161913
13	30.97677749838462	20.472390679859558	25.7585264649327	22.792305356823118
14	23.026320911150382	21.786957097148374	27.32205553782317	27.864666453878073
15	25.824854226837836	27.12556343565351	23.115225023938716	23.934357313569944
16	23.926728063961136	24.940016971187905	23.752968011646285	27.380286953204674
17	21.76188956271944	28.22417537932162	24.463422419094922	25.55051263886402
18	24.4216950947039	23.267965715086454	26.841568511439984	25.468770678769665
19	25.71243956933664	24.40425680988377	27.34245210310386	22.540851517675726
20	24.62488225265272	23.644134430492088	27.258686056378593	24.472297260476594
21	24.464045214981354	24.954964072462303	27.15841591866286	23.422574793893485
22	21.997306407791175	27.96882906588402	28.07999813161234	21.953866394712463
23	23.337096058480533	24.151557378963513	27.725471573260258	24.785874989295696
24	23.148388904891284	26.917549609584828	26.853557332253818	23.080504153270066
25	24.094882953298093	26.317797170949685	27.848940857745635	21.738379018006587
26	23.64117615003153	26.265170918546083	27.819825150054882	22.2738277813675
27	23.449510715981724	26.891547881326243	28.28988034534032	21.369061057351715
28	20.599596739663532	26.14590550629413	29.337734424264127	23.916763329778206
29	22.79246105579473	25.314472997905852	28.046055755801735	23.84701019049769
30	24.01687776852234	24.534888247063126	28.686289927055032	22.7619440573595
31	23.89558826963948	26.517403252551517	27.13833075132539	22.448677726483616
32	22.552373241574738	24.694012596046804	30.08213120752337	22.671482954855083
33	21.03041579410368	24.59747923364966	30.49956015040521	23.87254482184145
34	21.079305271188684	27.47557472382895	28.268082489315162	23.17703751566721
35	23.57360279635353	25.028453987061418	30.659774392190144	20.738168824394915
36	23.61190474336917	24.93223202260749	28.120635563202107	23.335227670821233
37	22.800401703346747	25.614972013109856	28.632106684935344	22.952519598608053
38	22.46954138867913	24.16370189874896	28.435458883794073	24.93129782877784
39	23.584190326422892	24.09612854507096	28.275244642009138	24.044436486497005
40	21.586883918631717	24.526947599511107	31.780962686741454	22.105205795115722
41	23.56254816936934	23.939651078604626	26.952270480253475	25.545530271772556
42	22.66338660833145	24.52289942624929	29.64383860244603	23.169875362973226
43	23.703767136618062	23.553984725930885	29.41246993063611	23.329778206814943
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	10.5
2	17.0
3	125.5
4	234.0
5	234.0
6	327.0
7	420.0
8	459.0
9	498.0
10	801.0
11	1104.0
12	1104.0
13	1776.0
14	2448.0
15	6218.0
16	9988.0
17	10026.0
18	10064.0
19	10064.0
20	8053.0
21	6042.0
22	4812.0
23	3582.0
24	3936.0
25	4290.0
26	4290.0
27	5177.0
28	6064.0
29	7528.0
30	8992.0
31	11186.5
32	13381.0
33	13381.0
34	17224.5
35	21068.0
36	23372.0
37	25676.0
38	30081.5
39	34487.0
40	34487.0
41	37945.0
42	41403.0
43	45046.0
44	48689.0
45	52431.0
46	56173.0
47	56173.0
48	60762.5
49	65352.0
50	71913.0
51	78474.0
52	66708.5
53	54943.0
54	54943.0
55	58439.5
56	61936.0
57	50861.5
58	39787.0
59	29598.5
60	19410.0
61	19410.0
62	16260.0
63	13110.0
64	10040.0
65	6970.0
66	5622.0
67	4274.0
68	4274.0
69	3196.5
70	2119.0
71	1506.0
72	893.0
73	539.0
74	185.0
75	185.0
76	144.5
77	104.0
78	71.5
79	39.0
80	30.5
81	22.0
82	22.0
83	17.0
84	12.0
85	7.5
86	3.0
87	4.0
88	5.0
89	5.0
90	4.0
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	642265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.812654653802339
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.184763596024716	9.991188461260808
2	12.44234061150969	3.934928703495651
3	5.394376866257327	2.558982553757628
4	3.2657629928179577	2.0656152954639335
5	2.1724496737614905	1.7176098221978002
6	1.5668784810667715	1.4865904983349933
7	1.193145796317869	1.3206761700167313
8	0.9862938752002834	1.2476739548562004
9	0.7804412521273772	1.1106763197724168
>10	7.024970553222879	23.69585027422895
>50	1.1581708209361483	12.896581189984554
>100	0.7704484063345731	22.46761197105412
>500	0.03997138294861599	4.2434503578548
>1k	0.013989984032015595	3.9552338822365853
>5k	0.004996422868576999	5.500447103218232
>10k+	9.992845737153996E-4	1.8068834422666036
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	11435	1.7804177403408252	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	9215	1.4347660233704156	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	7041	1.0962764590939877	TruSeq Adapter, Index 6 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6775	1.054860532646182	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6397	0.9960063213782473	No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	5382	0.8379718651958304	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	3084	0.48017562843997413	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	2740	0.42661518220672157	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	2679	0.4171175449386157	RNA PCR Primer, Index 6 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2320	0.36122161413123866	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	2211	0.34425042622593477	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1679	0.26141857333032315	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1480	0.23043447798027297	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1403	0.21844565716643444	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1319	0.20536694355133786	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1298	0.2020972651475637	No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG	1262	0.19649210216966514	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1240	0.19306672479428275	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	1170	0.18216779678170225	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	1146	0.17843102146310325	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	925	0.1440215487376706	No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA	920	0.14324305387962913	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	874	0.13608090118564767	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	861	0.13405681455473986	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	854	0.13296692175348182	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	841	0.13094283512257401	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	810	0.12611616700271694	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	805	0.12533767214467548	No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT	794	0.12362498345698428	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	793	0.12346928448537597	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	787	0.12253509065572622	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	774	0.12051100402481843	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	772	0.12019960608160184	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	767	0.11942111122356038	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	722	0.1124146575011872	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	710	0.1105462698418877	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	709	0.1103905708702794	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	695	0.1082107852677633	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.5569897160829253E-4	0.0
4	0.0	0.0	0.0	3.1139794321658506E-4	0.0
5	0.0	0.0	0.0	3.1139794321658506E-4	0.0
6	0.0	0.0	0.0	3.1139794321658506E-4	0.0
7	0.0	0.0	0.0	3.1139794321658506E-4	0.0
8	0.0	0.0	0.0	3.1139794321658506E-4	0.0
9	0.0	0.0	0.0	3.1139794321658506E-4	0.0
10	0.0	0.0	0.0	6.227958864331701E-4	0.0
11	0.0	0.0	0.0	7.784948580414627E-4	0.0
12	0.0	0.0	0.0	7.784948580414627E-4	0.0
13	0.0	0.0	0.0	7.784948580414627E-4	0.0
14	0.0	0.0	0.0	9.341938296497552E-4	0.0
15	0.0	0.0	0.0	9.341938296497552E-4	0.0
16	0.0	0.0	0.0	9.341938296497552E-4	0.0
17	0.0	0.0	0.0	9.341938296497552E-4	0.0
18	0.0	0.0	0.0	9.341938296497552E-4	0.0
19	0.0	0.0	0.0	9.341938296497552E-4	0.0
20	0.0	0.0	0.0	0.0012455917728663403	0.0
21	0.0	0.0	0.0	0.0015569897160829253	0.0
22	0.0	0.0	0.0	0.002024086630907803	0.0
23	0.0	0.0	0.0	0.002646882517340973	0.0
24	0.0	0.0	0.0	0.0034253773753824355	0.0
25	0.0	0.0	0.0	0.0037367753185990208	0.0
26	0.0	0.0	0.0	0.004982367091465361	0.0
27	0.0	0.0	0.0	0.006072259892723408	0.0
28	0.0	0.0	0.0	0.019462371451036565	0.0
29	0.0	0.0	0.0	0.0499793698862619	0.0
30	0.0	0.0	0.0	0.08781421998707699	0.0
31	0.0	0.0	0.0	0.16550800681961494	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAA	30	8.306273E-6	37.0	31
AGCAACG	20	0.0018413318	37.0	2
ATTACTT	50	1.8007995E-10	37.0	2
CGAGAAC	30	8.306273E-6	37.0	32
GATTACG	105	0.0	33.47619	1
CGTCTTC	375	0.0	32.066666	37
TTATACA	1460	0.0	31.931507	2
AGAACGA	35	2.38668E-5	31.714285	34
AACGAGA	35	2.38668E-5	31.714285	36
ACGAGAG	35	2.38668E-5	31.714285	37
TATACAC	1550	0.0	30.912905	3
CTTATAC	1530	0.0	30.591503	1
AAGCAAG	45	4.004638E-6	28.777777	32
GCAAAGC	40	5.936113E-5	27.75	22
ACCAAGC	40	5.936113E-5	27.75	35
AGTTTAG	55	6.2545223E-7	26.90909	9
CGACCCA	35	8.865119E-4	26.42857	22
GGTATCA	730	0.0	25.59589	1
GTATCAA	2435	0.0	25.147842	1
CCGTCTT	1210	0.0	25.074379	37
>>END_MODULE