FastQCFastQC Report
Fri 10 Feb 2017
ERR1630927.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630927.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000291
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT222492.2242527424519465No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG177751.7769828979766886No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT147461.4741710162342758Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC93230.9320287796251291No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC59400.5938271962858809Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC42110.4209774955487953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27850.27841898007679766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26670.26662241287785254No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG21500.21493745320111846No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA17240.17234984619475732No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT14390.14385813728205094No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT11090.11086773748839088No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT10570.10566925024817779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10470.10466954116352142No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10350.10346989026193378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC25100.033.6095621
GATTACG1000.033.31
CGTCTTC7250.031.13103537
TTATACA27700.030.6552352
TATACAC28500.030.3789483
GGTATCA6150.027.3739831
CCGTCTT22300.025.05381237
GCCGTCT23200.024.08189636
TCGCCGA551.9023391E-523.5454541
GTATCAA15650.023.0511191
TTACGGG1500.022.23
ATTACGG1550.021.4838712
AACGGCC909.475116E-820.55555737
CGCAGCC450.003825465520.55555728
ATGCGCT450.003825465520.55555731
TGCCGTC28100.020.01423535
TTAACGG851.2449782E-619.58823635
ATGCCGT29250.019.2273534
AGGACCG701.2189965E-418.55
CGAACTA1002.8740033E-718.49999829
Produced by FastQC (version 0.11.5)