FastQCFastQC Report
Fri 10 Feb 2017
ERR1630926.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630926.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277608
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT176076.342396472724129No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG138744.997694590933978No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT135914.895752283795856TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC71172.563686925448834No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC67232.421760179821907RNA PCR Primer, Index 3 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42581.5338174692371978No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC40551.4606927754243393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39781.4329558225987724No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23020.8292268234344832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16100.5799544681709461No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA15510.5587014783435637No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG14840.5345667271836546No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14440.5201579205210224No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12710.45783983170513814No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT12500.4502752082072563No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12360.445232125875335No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10790.38867755972450363No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10430.3757096337281346No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9550.34401025907034377No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9510.3425693784040806No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT8950.3223970490763955No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8820.31771418691104003No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8490.30582692141436846No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6640.23918619059969454No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6250.22513760410362815No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6250.22513760410362815No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6210.22369672343736494No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6180.2226160629376675No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6080.21901386127200945No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5930.2136105587735224No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5890.21216967810725917No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.20640615544220628No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5720.20604593527564047No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5600.20172329327685082No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5590.20136307311028498No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5570.20064263277715338No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4910.17686810178381027No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4770.171825019451889No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4760.17146479928532318No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4720.17002391861905997No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT4590.1653410564537045No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4420.15921731362208583No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4320.15561511195642777No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4260.15345379095703293No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4210.1516526901242039No Hit
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT4040.14552894729258523TruSeq Adapter, Index 3 (95% over 23bp)
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3970.1430074061266246No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3930.1415665254603614No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3880.13976542462753233No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3710.13364168179591365No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3680.13256102129621625No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3660.13184058096308465No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3660.13184058096308465No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3620.1303997002968214No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3330.11995331546641307No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3320.11959309529984727No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3310.11923287513328146No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3290.11851243480014986No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3290.11851243480014986No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.11743177430045244No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3260.11743177430045244No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3240.11671133396732082No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3200.1152704533010576No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3100.11166825163539955No Hit
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA3080.11094781130226794No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA3050.10986715080257053No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3000.10806604996974149No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3000.10806604996974149No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2990.1077058298031757No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2880.10374340797095186No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2870.10338318780438603No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2870.10338318780438603No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2850.10266274747125442No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2830.10194230713812281No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2790.1005014264718596No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGA200.001839532337.01
CAGACAC200.001839532337.018
CTTATAC19900.036.070351
CGTCTTC7750.035.09032437
TTATACA20900.034.34452
CTATACA750.032.0666661
TGATACA352.3818351E-531.7142852
TATACAC23200.031.0193963
CCGGAAA303.5925105E-430.83333434
GACAGAC250.005489254429.59999816
CGTTATT250.005489254429.59999810
TCAGAGT250.005489254429.5999983
GAAAATC250.005489254429.59999837
GGAAAAT250.005489254429.59999836
ACTCAGA250.005489254429.5999981
CGCCGAG250.005489254429.5999982
GTTATTG250.005489254429.59999811
GATACAC405.924158E-527.753
ACAGTTG358.8523893E-426.4285719
GGCAAAT358.8523893E-426.4285727
Produced by FastQC (version 0.11.5)