##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630926.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 277608 Sequences flagged as poor quality 0 Sequence length 43 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.186503991239444 31.0 31.0 34.0 28.0 34.0 2 31.208120803435058 31.0 31.0 34.0 28.0 34.0 3 30.644905766404428 31.0 31.0 34.0 26.0 34.0 4 34.800099420765974 37.0 35.0 37.0 32.0 37.0 5 35.04766793464165 35.0 35.0 37.0 32.0 37.0 6 35.243148612431916 37.0 35.0 37.0 32.0 37.0 7 35.22215498112446 37.0 35.0 37.0 32.0 37.0 8 35.251282383792976 37.0 35.0 37.0 32.0 37.0 9 37.05583772801936 39.0 37.0 39.0 33.0 39.0 10 36.889437624275956 39.0 37.0 39.0 33.0 39.0 11 36.9419217025446 39.0 37.0 39.0 33.0 39.0 12 36.82472046915075 39.0 37.0 39.0 32.0 39.0 13 36.94671983516325 39.0 37.0 39.0 33.0 39.0 14 37.75078527996311 39.0 37.0 41.0 32.0 41.0 15 37.79790928215325 39.0 37.0 41.0 33.0 41.0 16 37.79814342526152 39.0 37.0 41.0 33.0 41.0 17 37.7558211578917 39.0 37.0 41.0 32.0 41.0 18 37.91966009625083 40.0 37.0 41.0 33.0 41.0 19 37.81399311259042 39.0 37.0 41.0 33.0 41.0 20 37.75843635630097 39.0 37.0 41.0 32.0 41.0 21 37.76311921846633 39.0 37.0 41.0 33.0 41.0 22 37.59077908417625 39.0 36.0 41.0 32.0 41.0 23 37.64981916947639 39.0 37.0 41.0 32.0 41.0 24 37.530503443704795 39.0 36.0 41.0 32.0 41.0 25 37.334957926284545 39.0 36.0 41.0 32.0 41.0 26 37.17066511051555 39.0 36.0 40.0 31.0 41.0 27 36.895489323074266 39.0 35.0 40.0 31.0 41.0 28 36.98799386184836 39.0 35.0 40.0 31.0 41.0 29 36.985710065992336 39.0 35.0 40.0 31.0 41.0 30 36.5207054551742 38.0 35.0 40.0 30.0 41.0 31 36.527812599060546 38.0 35.0 40.0 30.0 41.0 32 36.3420326503559 38.0 35.0 40.0 30.0 41.0 33 36.121253710267716 38.0 35.0 40.0 30.0 41.0 34 36.093790524768735 38.0 35.0 40.0 30.0 41.0 35 36.04244474222645 38.0 35.0 40.0 30.0 41.0 36 35.875860926198094 38.0 35.0 40.0 29.0 41.0 37 35.80832324716867 38.0 35.0 40.0 29.0 41.0 38 35.737705685715106 38.0 35.0 40.0 28.0 41.0 39 35.47394527535229 38.0 34.0 40.0 27.0 41.0 40 35.12457494020345 38.0 34.0 40.0 26.0 41.0 41 35.04516080228235 38.0 34.0 40.0 26.0 41.0 42 34.852954525806176 38.0 34.0 40.0 24.0 41.0 43 33.81109334024956 37.0 33.0 40.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 9.0 10 14.0 11 26.0 12 16.0 13 14.0 14 8.0 15 9.0 16 19.0 17 14.0 18 22.0 19 35.0 20 67.0 21 146.0 22 255.0 23 472.0 24 822.0 25 1359.0 26 2110.0 27 3069.0 28 4272.0 29 5659.0 30 7173.0 31 8855.0 32 11066.0 33 13851.0 34 17484.0 35 22924.0 36 30517.0 37 43007.0 38 60642.0 39 43668.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.24793233624391 21.64851157027175 17.812166796345927 26.291389297138412 2 12.155989740929657 21.195354600731967 43.226419987896605 23.422235670441776 3 14.102619521051265 29.986167545603877 36.82062476585689 19.09058816748797 4 9.338707818218495 20.985706463790667 28.830221031094204 40.84536468689663 5 14.047505835566698 36.47517362612028 30.3651191608311 19.112201377481917 6 25.83318924526671 38.18333765597533 15.101149822771678 20.882323275986284 7 22.48998587936947 33.749747845883405 15.560790755309645 28.199475519437478 8 27.816201262211464 28.94657214489496 23.467623411429063 19.76960318146451 9 20.613599031728192 11.168626264372785 14.895464107662605 53.32231059623642 10 15.59897409296562 27.296763782023575 26.85729517881329 30.246966946197517 11 29.798853058989653 20.240771159332585 22.95286879340653 27.007506988271228 12 19.049883288666035 26.744906486844762 21.643828708106394 32.561381516382816 13 35.472680902567646 16.124175095818565 24.741001700239188 23.6621423013746 14 23.34803031612922 24.848707530042365 22.999697415060087 28.803564738768333 15 28.900463963574534 24.789991642892137 19.022146335840464 27.28739805769286 16 29.35362093311432 22.34229561107749 18.44723495000144 29.85684850580675 17 18.825466124895538 33.555949396271004 19.05096394916573 28.56762052966773 18 29.4728538082476 16.262139419613266 22.07861444915132 32.186392322987814 19 29.067245900694505 23.342987233797295 24.4005936428345 23.1891732226737 20 20.56460908907524 20.933474539638627 27.464626379643235 31.03728999164289 21 31.286202126739866 23.041122734215154 22.328247024581422 23.34442811446356 22 23.601625313391544 35.23421515230108 23.58901760756174 17.575141926745626 23 27.218596005878794 24.130428517910147 22.985648828564017 25.665326647647042 24 36.564148007262034 23.030676349384745 23.02671392755252 17.378461715800697 25 23.982378029451603 30.163756087720817 25.033860695657182 20.8200051871704 26 32.53652632488978 29.08921933085502 21.787916774732714 16.58633756952249 27 23.458978127431486 29.686104146854557 30.73794703322671 16.116970692487246 28 17.313261865652287 28.56077634650298 28.199475519437478 25.92648626840725 29 33.60241779775799 19.924858073254374 23.842252384657503 22.630471744330137 30 19.835523471946054 25.267643583758392 36.33828996282528 18.558542981470275 31 28.625615976484823 26.75895507334082 21.39599723350912 23.219431716665227 32 33.265611942018964 21.000475490619866 27.026238436932655 18.70767413042852 33 18.21561338289963 32.365061525604446 30.91877755684274 18.50054753465318 34 24.379700873173686 28.556453704504193 25.455318290539175 21.608527131782946 35 25.973314890060806 20.758767758854212 36.29902596466961 16.96889138641538 36 23.000417855393216 27.00318434627244 23.037520532549493 26.958877265784846 37 21.188510417567215 25.401285265554307 28.592475721160778 24.817728595717703 38 21.08692833059566 18.18463444857497 30.640327368087373 30.088109852741994 39 28.196593758104953 19.15939021930204 26.18440390766837 26.459612114924642 40 18.13456384542232 19.22711161061641 40.463531310336876 22.174793233624392 41 23.59766289155932 21.271361055877353 24.626811907437826 30.5041641451255 42 24.21616091755281 18.42922394167315 35.982392438258266 21.372222702515778 43 23.866026915650846 18.60897380478949 33.29226823434483 24.232731045214837 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 7.0 2 12.0 3 77.5 4 143.0 5 143.0 6 229.0 7 315.0 8 346.0 9 377.0 10 590.5 11 804.0 12 804.0 13 1221.5 14 1639.0 15 4015.0 16 6391.0 17 6430.0 18 6469.0 19 6469.0 20 5295.0 21 4121.0 22 3217.0 23 2313.0 24 2168.5 25 2024.0 26 2024.0 27 2004.0 28 1984.0 29 1964.5 30 1945.0 31 1981.0 32 2017.0 33 2017.0 34 2366.0 35 2715.0 36 2681.5 37 2648.0 38 3002.0 39 3356.0 40 3356.0 41 4295.5 42 5235.0 43 7096.0 44 8957.0 45 12385.5 46 15814.0 47 15814.0 48 21405.5 49 26997.0 50 41223.0 51 55449.0 52 41015.5 53 26582.0 54 26582.0 55 35895.0 56 45208.0 57 37708.0 58 30208.0 59 20060.0 60 9912.0 61 9912.0 62 8032.0 63 6152.0 64 4870.0 65 3588.0 66 2950.0 67 2312.0 68 2312.0 69 1808.5 70 1305.0 71 879.5 72 454.0 73 267.0 74 80.0 75 80.0 76 57.5 77 35.0 78 31.0 79 27.0 80 19.5 81 12.0 82 12.0 83 8.0 84 4.0 85 2.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 277608.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.568989366300684 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0828726066461 13.385060949252184 2 10.937166579371084 4.061842598196018 3 4.33762051640187 2.41635687732342 4 2.5820093503268735 1.917812166796346 5 1.621757939048284 1.5057202962450649 6 1.2395972763778154 1.3810841186132965 7 0.8399774971386448 1.0918273248609551 8 0.6401676075190595 0.9509812397337253 9 0.5024345768104134 0.8396732082648914 >10 4.1766086636016215 15.516843894988616 >50 0.5819705522900541 7.607489697703236 >100 0.3879803681933694 14.106942163050057 >500 0.03491823313740325 4.429267168093139 >1k 0.025218723932569014 9.567807844154347 >5k 0.0038798036819336942 4.985447105270741 >10k+ 0.005819705522900541 16.23584334745396 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT 17607 6.342396472724129 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG 13874 4.997694590933978 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 13591 4.895752283795856 TruSeq Adapter, Index 3 (95% over 21bp) ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC 7117 2.563686925448834 No Hit CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC 6723 2.421760179821907 RNA PCR Primer, Index 3 (95% over 22bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 4258 1.5338174692371978 No Hit CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC 4055 1.4606927754243393 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 3978 1.4329558225987724 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 2302 0.8292268234344832 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1610 0.5799544681709461 No Hit TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA 1551 0.5587014783435637 No Hit CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG 1484 0.5345667271836546 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 1444 0.5201579205210224 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT 1271 0.45783983170513814 No Hit TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT 1250 0.4502752082072563 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 1236 0.445232125875335 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 1079 0.38867755972450363 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 1043 0.3757096337281346 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC 955 0.34401025907034377 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG 951 0.3425693784040806 No Hit ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 895 0.3223970490763955 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 882 0.31771418691104003 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG 849 0.30582692141436846 No Hit GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG 664 0.23918619059969454 No Hit GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG 625 0.22513760410362815 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG 625 0.22513760410362815 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG 621 0.22369672343736494 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 618 0.2226160629376675 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 608 0.21901386127200945 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG 593 0.2136105587735224 No Hit GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG 589 0.21216967810725917 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 573 0.20640615544220628 No Hit GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG 572 0.20604593527564047 No Hit GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG 560 0.20172329327685082 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 559 0.20136307311028498 No Hit GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG 557 0.20064263277715338 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG 491 0.17686810178381027 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 477 0.171825019451889 No Hit GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG 476 0.17146479928532318 No Hit ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA 472 0.17002391861905997 No Hit ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT 459 0.1653410564537045 No Hit GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC 442 0.15921731362208583 No Hit AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT 432 0.15561511195642777 No Hit GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA 426 0.15345379095703293 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC 421 0.1516526901242039 No Hit TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 404 0.14552894729258523 TruSeq Adapter, Index 3 (95% over 23bp) GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC 397 0.1430074061266246 No Hit TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG 393 0.1415665254603614 No Hit CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT 388 0.13976542462753233 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC 371 0.13364168179591365 No Hit CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG 368 0.13256102129621625 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC 366 0.13184058096308465 No Hit ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC 366 0.13184058096308465 No Hit GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA 362 0.1303997002968214 No Hit GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG 333 0.11995331546641307 No Hit CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG 332 0.11959309529984727 No Hit CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG 331 0.11923287513328146 No Hit GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG 329 0.11851243480014986 No Hit TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA 329 0.11851243480014986 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 326 0.11743177430045244 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA 326 0.11743177430045244 No Hit GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG 324 0.11671133396732082 No Hit ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG 320 0.1152704533010576 No Hit CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC 310 0.11166825163539955 No Hit CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA 308 0.11094781130226794 No Hit TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA 305 0.10986715080257053 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT 300 0.10806604996974149 No Hit GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA 300 0.10806604996974149 No Hit GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG 299 0.1077058298031757 No Hit ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC 288 0.10374340797095186 No Hit GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA 287 0.10338318780438603 No Hit ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA 287 0.10338318780438603 No Hit GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA 285 0.10266274747125442 No Hit CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA 283 0.10194230713812281 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA 279 0.1005014264718596 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.60220166565805E-4 0.0 6 0.0 0.0 0.0 3.60220166565805E-4 0.0 7 0.0 0.0 0.0 0.001080660499697415 0.0 8 0.0 0.0 0.0 0.001080660499697415 0.0 9 0.0 0.0 0.0 0.001801100832829025 0.0 10 0.0 0.0 0.0 0.00216132099939483 0.0 11 0.0 0.0 0.0 0.00216132099939483 0.0 12 0.0 0.0 0.0 0.00216132099939483 0.0 13 0.0 0.0 0.0 0.002521541165960635 0.0 14 0.0 0.0 0.0 0.00288176133252644 0.0 15 0.0 0.0 0.0 0.00288176133252644 0.0 16 0.0 0.0 0.0 0.003241981499092245 0.0 17 0.0 0.0 0.0 0.00360220166565805 0.0 18 0.0 0.0 0.0 0.00432264199878966 0.0 19 0.0 0.0 0.0 0.005403302498487075 0.0 20 0.0 0.0 0.0 0.00576352266505288 0.0 21 0.0 0.0 0.0 0.006844183164750295 0.0 22 0.0 0.0 0.0 0.007924843664447711 0.0 23 0.0 0.0 0.0 0.01008616466384254 0.0 24 0.0 0.0 0.0 0.013328146162934786 0.0 25 0.0 0.0 0.0 0.014048586496066396 0.0 26 0.0 0.0 0.0 0.014769026829198006 0.0 27 0.0 0.0 0.0 0.01729056799515864 0.0 28 0.0 0.0 0.0 0.0356617964900147 0.0 29 0.0 0.0 0.0 0.07708711564508228 0.0 30 0.0 0.0 0.0 0.12535661796490014 0.0 31 0.0 0.0 0.0 0.1941586697789689 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCGA 20 0.0018395323 37.0 1 CAGACAC 20 0.0018395323 37.0 18 CTTATAC 1990 0.0 36.07035 1 CGTCTTC 775 0.0 35.090324 37 TTATACA 2090 0.0 34.3445 2 CTATACA 75 0.0 32.066666 1 TGATACA 35 2.3818351E-5 31.714285 2 TATACAC 2320 0.0 31.019396 3 CCGGAAA 30 3.5925105E-4 30.833334 34 GACAGAC 25 0.0054892544 29.599998 16 CGTTATT 25 0.0054892544 29.599998 10 TCAGAGT 25 0.0054892544 29.599998 3 GAAAATC 25 0.0054892544 29.599998 37 GGAAAAT 25 0.0054892544 29.599998 36 ACTCAGA 25 0.0054892544 29.599998 1 CGCCGAG 25 0.0054892544 29.599998 2 GTTATTG 25 0.0054892544 29.599998 11 GATACAC 40 5.924158E-5 27.75 3 ACAGTTG 35 8.8523893E-4 26.42857 19 GGCAAAT 35 8.8523893E-4 26.42857 27 >>END_MODULE