FastQCFastQC Report
Fri 10 Feb 2017
ERR1630925.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630925.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974994
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT97240.9973394708069998No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG82400.8451334059491648No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT70570.7237993259445699RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC47790.4901568625037693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26510.2718991091227228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25240.2588733879387976No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC24870.25507849279072486RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC22960.23548862864797115No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG11500.11794944379144898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG658.731149E-1131.3076921
CTTATAC13700.029.4379561
TTATACA14250.028.3017542
TATACAC15950.026.0971783
GGTATCA5350.025.9345781
GTATCAA16000.024.2812521
CCGTCTT11200.024.2812537
GCCGTCT11250.024.00888836
CGTCTTC4250.021.76470637
GTACGGT450.003825397720.55555511
CCGGCGT555.142325E-420.18181823
GCAACGC555.142325E-420.1818183
ATTTCGG656.900756E-519.92307729
TGCCGTC14100.019.15602935
CGGTCGT1002.8738032E-718.510
ATGCCGT15200.017.89144734
TACACAT24300.017.2818935
TCGGGAT752.0668609E-417.26666622
AACGGCC650.001579701317.07692337
CTAACCG650.001579701317.0769236