##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630925.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	974994
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47325214309011	31.0	31.0	34.0	28.0	34.0
2	31.468452113551468	31.0	31.0	34.0	28.0	34.0
3	30.93713294645916	31.0	31.0	34.0	27.0	34.0
4	35.09548366451486	37.0	35.0	37.0	32.0	37.0
5	35.28837408230204	37.0	35.0	37.0	33.0	37.0
6	35.478562945002736	37.0	35.0	37.0	33.0	37.0
7	35.487956848965226	37.0	35.0	37.0	33.0	37.0
8	35.51350572413779	37.0	35.0	37.0	33.0	37.0
9	37.281679682131376	39.0	37.0	39.0	34.0	39.0
10	37.10791451024314	39.0	37.0	39.0	33.0	39.0
11	37.171582594354426	39.0	37.0	39.0	33.0	39.0
12	37.11173094398529	39.0	37.0	39.0	33.0	39.0
13	37.177367245336896	39.0	37.0	39.0	33.0	39.0
14	38.33799797742345	40.0	38.0	41.0	34.0	41.0
15	38.33696207361276	40.0	38.0	41.0	34.0	41.0
16	38.3381559271134	40.0	38.0	41.0	34.0	41.0
17	38.31729118333036	40.0	38.0	41.0	33.0	41.0
18	38.34523494503556	40.0	38.0	41.0	34.0	41.0
19	38.382063889623936	40.0	38.0	41.0	34.0	41.0
20	38.387470076738936	40.0	38.0	41.0	34.0	41.0
21	38.346246233310154	40.0	38.0	41.0	34.0	41.0
22	38.28825203026891	40.0	38.0	41.0	34.0	41.0
23	38.27205911010734	40.0	38.0	41.0	34.0	41.0
24	38.24322611216069	40.0	38.0	41.0	34.0	41.0
25	38.22117674570305	40.0	38.0	41.0	34.0	41.0
26	38.114727885504934	40.0	37.0	41.0	33.0	41.0
27	38.06484450160719	40.0	37.0	41.0	33.0	41.0
28	38.03357764252908	40.0	37.0	41.0	33.0	41.0
29	37.96796082847689	40.0	37.0	41.0	33.0	41.0
30	37.8963603878588	40.0	37.0	41.0	33.0	41.0
31	37.87103407815843	40.0	37.0	41.0	33.0	41.0
32	37.796186438070386	40.0	37.0	41.0	33.0	41.0
33	37.77205500751799	40.0	37.0	41.0	33.0	41.0
34	37.709331544604375	40.0	37.0	41.0	33.0	41.0
35	37.67881135678784	40.0	37.0	41.0	33.0	41.0
36	37.66062150126052	40.0	37.0	41.0	33.0	41.0
37	37.61273300143385	40.0	37.0	41.0	32.0	41.0
38	37.58471026488368	40.0	37.0	41.0	32.0	41.0
39	37.513296492080975	40.0	37.0	41.0	32.0	41.0
40	37.45090533890465	40.0	37.0	41.0	32.0	41.0
41	37.396321413259976	39.0	37.0	41.0	32.0	41.0
42	37.34342160054318	39.0	36.0	41.0	31.0	41.0
43	36.74970102380117	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	8.0
11	8.0
12	12.0
13	3.0
14	3.0
15	4.0
16	11.0
17	12.0
18	12.0
19	39.0
20	79.0
21	139.0
22	322.0
23	557.0
24	1079.0
25	1933.0
26	3289.0
27	5060.0
28	7679.0
29	11274.0
30	15228.0
31	20393.0
32	27190.0
33	35462.0
34	47348.0
35	64068.0
36	89266.0
37	134098.0
38	235457.0
39	274956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.63213517211388	22.420137970079814	14.93906629169	24.008660566116305
2	13.95075251745139	22.387830078954334	40.62732693739653	23.03409046619774
3	14.64193625806928	28.547662857412455	33.72133572104033	23.08906516347793
4	11.789918707192044	18.875500772312446	39.49757639534192	29.837004125153594
5	12.401717343901604	38.519621659210216	36.08494000988724	12.993720987000945
6	27.867966366972514	40.68178881100807	17.97487984541443	13.475364976604984
7	24.837999003070788	33.13189619628428	23.78127455143314	18.24883024921179
8	24.78794741300972	35.57621893057804	21.846288284850985	17.78954537156126
9	24.710818733243485	15.346248284604828	20.38802290065375	39.55491008149794
10	14.401114263267262	28.900485541449484	34.45436587302076	22.244034322262497
11	31.706759221082386	23.68876116160715	25.55779830439982	19.046681312910643
12	20.96956494091246	26.76621599722665	30.414443576063032	21.849775485797863
13	28.425405694804272	21.66833847182649	26.83780618137137	23.06844965199786
14	21.53992742519441	21.88772443727859	27.655041979745516	28.917306157781486
15	23.15511685200114	29.480796804903413	23.733889644449093	23.63019669864635
16	23.50137539307934	28.07791637692129	24.854819619402786	23.565888610596577
17	21.12946336080017	29.840081067165542	26.127032576610727	22.90342299542356
18	23.238707109992472	26.97329419463094	26.712164382550046	23.07583431282654
19	23.47327265603686	27.366835077959458	26.748472298291066	22.41141996771262
20	22.7967556723426	26.742933802669555	26.888370595101097	23.57193992988675
21	23.287220229047563	26.808780361725304	27.306834708726413	22.59716470050072
22	21.52095294945405	29.869619710480272	26.888575724568565	21.72085161549712
23	22.526600163693313	27.109295031559167	27.299860306832656	23.06424449791486
24	22.866909950215078	28.155968139291115	26.626010006215424	22.351111904278383
25	21.687312947566856	28.71884339801065	26.988371210489497	22.60547244393299
26	22.928448790454095	27.111756585168727	27.025807338301572	22.933987286075606
27	21.448234553238276	29.223461887970593	26.603856023729378	22.724447535061753
28	21.63367159182518	26.960883861849403	28.505098492913806	22.90034605341161
29	22.357676047237213	27.61883662873823	26.83411385095703	23.189373473067526
30	21.684236005554904	27.927453912536897	27.39042496671774	22.997885115190453
31	23.33604104230385	26.399547074135842	27.616272510394936	22.648139373165375
32	21.93613499159995	28.930639573166605	27.532887381871067	21.600338053362382
33	21.54946594543146	27.716067996315875	28.196173514913937	22.538292543338727
34	23.030910959452058	27.645606024242202	27.196167361029914	22.12731565527583
35	21.684338570288638	27.63124696151976	29.122025366309945	21.562389101881653
36	22.870294586428226	26.783344307759844	26.220058790105377	24.126302315706557
37	22.278496072796344	27.148269630377214	27.89965886969561	22.673575427130835
38	22.342496466644924	26.375854620643818	27.99842870827923	23.283220204432027
39	23.260245704076127	26.500265642660363	27.400886569558374	22.83860208370513
40	21.584235390166505	26.12734027081192	30.081723579837416	22.20670075918416
41	22.411009708777698	26.30949523791941	27.246936904227105	24.03255814907579
42	22.225162411255862	26.38262389307011	28.834741547127468	22.557472148546555
43	22.601062160382526	25.73195322227624	28.83853644227554	22.828448175065695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	21.5
2	32.0
3	94.0
4	156.0
5	156.0
6	204.0
7	252.0
8	262.0
9	272.0
10	430.0
11	588.0
12	588.0
13	922.5
14	1257.0
15	2836.0
16	4415.0
17	5168.0
18	5921.0
19	5921.0
20	6522.0
21	7123.0
22	9246.0
23	11369.0
24	14749.0
25	18129.0
26	18129.0
27	22883.5
28	27638.0
29	32099.5
30	36561.0
31	41160.5
32	45760.0
33	45760.0
34	50453.5
35	55147.0
36	58568.0
37	61989.0
38	65055.0
39	68121.0
40	68121.0
41	69525.5
42	70930.0
43	71158.5
44	71387.0
45	71478.0
46	71569.0
47	71569.0
48	70551.5
49	69534.0
50	75785.0
51	82036.0
52	70766.5
53	59497.0
54	59497.0
55	61797.0
56	64097.0
57	55545.5
58	46994.0
59	38695.0
60	30396.0
61	30396.0
62	27163.5
63	23931.0
64	19869.5
65	15808.0
66	13005.0
67	10202.0
68	10202.0
69	8369.5
70	6537.0
71	5066.5
72	3596.0
73	2760.0
74	1924.0
75	1924.0
76	1444.0
77	964.0
78	723.5
79	483.0
80	347.5
81	212.0
82	212.0
83	149.5
84	87.0
85	64.0
86	41.0
87	31.0
88	21.0
89	21.0
90	13.0
91	5.0
92	3.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	974994.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.694779159112144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.01683020787901	46.16553347855753
2	12.142633253446915	14.011330879354222
3	3.492785937812256	6.045465399963915
4	1.375561985136651	3.1745097980851593
5	0.7106041487907218	2.049907471701478
6	0.4202717847853398	1.4548492685997652
7	0.2890908710558795	1.167532377373903
8	0.21605812736789592	0.9972340755217662
9	0.17099473970996984	0.8878953370442916
>10	1.0209713892890695	11.432600931259966
>50	0.09690984733930455	3.8207512712065954
>100	0.04478948898992308	4.214482924655938
>500	8.922208561157279E-4	0.36630666907280734
>1k	0.0010706650273388733	1.6356138424844548
>5k	5.353325136694366E-4	2.575986275118244
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	9724	0.9973394708069998	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	8240	0.8451334059491648	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	7057	0.7237993259445699	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	4779	0.4901568625037693	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2651	0.2718991091227228	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2524	0.2588733879387976	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	2487	0.25507849279072486	RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	2296	0.23548862864797115	No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG	1150	0.11794944379144898	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0256473373169476E-4	0.0
3	0.0	0.0	0.0	1.0256473373169476E-4	0.0
4	0.0	0.0	0.0	1.0256473373169476E-4	0.0
5	0.0	0.0	0.0	1.0256473373169476E-4	0.0
6	0.0	0.0	0.0	1.0256473373169476E-4	0.0
7	0.0	0.0	0.0	1.0256473373169476E-4	0.0
8	0.0	0.0	0.0	1.0256473373169476E-4	0.0
9	0.0	0.0	0.0	1.0256473373169476E-4	0.0
10	0.0	0.0	0.0	1.0256473373169476E-4	0.0
11	0.0	0.0	0.0	2.0512946746338952E-4	0.0
12	0.0	0.0	0.0	2.0512946746338952E-4	0.0
13	0.0	0.0	0.0	3.0769420119508426E-4	0.0
14	0.0	0.0	0.0	3.0769420119508426E-4	0.0
15	0.0	0.0	0.0	4.1025893492677905E-4	0.0
16	0.0	0.0	0.0	4.1025893492677905E-4	0.0
17	0.0	0.0	0.0	4.1025893492677905E-4	0.0
18	0.0	0.0	0.0	6.153884023901685E-4	0.0
19	0.0	0.0	0.0	6.153884023901685E-4	0.0
20	0.0	0.0	0.0	6.153884023901685E-4	0.0
21	0.0	0.0	0.0	0.0010256473373169476	0.0
22	0.0	0.0	0.0	0.0013333415385120319	0.0
23	0.0	0.0	0.0	0.0015384710059754214	0.0
24	0.0	0.0	0.0	0.002256424142097285	0.0
25	0.0	0.0	0.0	0.0023589888758289795	0.0
26	0.0	0.0	0.0	0.002564118343292369	0.0
27	0.0	0.0	0.0	0.0031795067456825373	0.0
28	0.0	0.0	0.0	0.00882056710092575	0.0
29	0.0	0.0	0.0	0.022769370888436235	0.0
30	0.0	0.0	0.0	0.04010281088909265	0.0
31	0.0	0.0	0.0	0.09097491882001325	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	65	8.731149E-11	31.307692	1
CTTATAC	1370	0.0	29.437956	1
TTATACA	1425	0.0	28.301754	2
TATACAC	1595	0.0	26.097178	3
GGTATCA	535	0.0	25.934578	1
GTATCAA	1600	0.0	24.281252	1
CCGTCTT	1120	0.0	24.28125	37
GCCGTCT	1125	0.0	24.008888	36
CGTCTTC	425	0.0	21.764706	37
GTACGGT	45	0.0038253977	20.555555	11
CCGGCGT	55	5.142325E-4	20.181818	23
GCAACGC	55	5.142325E-4	20.181818	3
ATTTCGG	65	6.900756E-5	19.923077	29
TGCCGTC	1410	0.0	19.156029	35
CGGTCGT	100	2.8738032E-7	18.5	10
ATGCCGT	1520	0.0	17.891447	34
TACACAT	2430	0.0	17.281893	5
TCGGGAT	75	2.0668609E-4	17.266666	22
AACGGCC	65	0.0015797013	17.076923	37
CTAACCG	65	0.0015797013	17.076923	6
>>END_MODULE