FastQCFastQC Report
Fri 10 Feb 2017
ERR1630924.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630924.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698781
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT148272.1218378862619334No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG130021.8606687932270625No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT118421.6946654245035282RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC68940.9865751930862459No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC46940.6717412179209223RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC36120.5169001446805223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22160.3171236768028896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22100.31626503868880235No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG15710.22482007953851063No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA13730.19648502177363153No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT10980.15713077487796606No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT9440.13509239661639338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8790.12579048371378157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19150.033.9086151
CGTCTTC6000.031.75833337
GGATCGT352.3869776E-531.7142876
TTATACA20750.031.3831332
TATACAC22800.029.4539473
ATCGTCG358.8659E-426.4285728
CGTCGCC358.8659E-426.42857210
GGTATCA3950.025.291141
CCGTCTT18400.024.8342437
GTATCAA12100.024.4628091
GCCGTCT18650.024.40214336
TCGTCGC400.001930433723.1259
GATTACG400.001930433723.1251
CCGTTAC400.001930433723.12525
CTTAGAC502.7006326E-422.23
GGGATCG705.0971994E-621.1428595
AGCCGTT450.00382432920.55555723
GATCGTC555.140322E-420.1818187
TGCCGTC22700.020.04845835
CGTATAC656.897181E-519.9230771
Produced by FastQC (version 0.11.5)