FastQCFastQC Report
Fri 10 Feb 2017
ERR1630923.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630923.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810271
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG106361.3126472501175532No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT99431.2271203091311425No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT79710.9837449445926115TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC59870.738888594062974No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC28590.35284491238116633No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC27120.33470283399010947Illumina Paired End PCR Primer 2 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26320.3248295940493983No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25340.3127348751220271No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG14150.17463293145132924No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT13410.16550018450617138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9060.11181444232855427No Hit
GGGATATACAAAGTGGAAATAGACACCAAATCTTACTGGAAGG9000.11107394933300094No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA8530.10527342086783312No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT8140.1004602163967364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG200.001841615537.02
GATTACG658.731149E-1131.3076921
CTTATAC13550.030.7195591
GGTATCA3900.028.461541
TTATACA15200.027.628292
TATACAC15650.027.4249213
CGTCTTC3850.027.3896137
CCGTCTT12350.025.61538537
GTATCAA13600.025.43751
GCCGTCT12800.024.71484436
GCAACGC601.3365934E-624.6666683
TCGCCGA502.7011495E-422.1999991
AGCAACG705.0988565E-621.1428592
TTACGGG909.469659E-820.5555553
TGCCGTC15550.020.34405335
ATGCCGT16350.019.34862534
CGGTACT1153.0468073E-919.3043464
ATTACGG1103.8473445E-818.52
CGCCGAG609.2336064E-418.52
TCTATAC1306.9485395E-1018.53
Produced by FastQC (version 0.11.5)