##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630923.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	810271
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.047211365086497	31.0	31.0	34.0	28.0	34.0
2	31.0372023680966	31.0	31.0	34.0	28.0	34.0
3	30.444547564950494	31.0	30.0	34.0	26.0	34.0
4	34.692900276574136	35.0	35.0	37.0	32.0	37.0
5	34.83728777162209	35.0	35.0	37.0	32.0	37.0
6	35.05882106110178	36.0	35.0	37.0	32.0	37.0
7	35.07622141234229	36.0	35.0	37.0	32.0	37.0
8	35.11083945988441	37.0	35.0	37.0	32.0	37.0
9	36.8221644363429	39.0	37.0	39.0	33.0	39.0
10	36.59782838087504	38.0	35.0	39.0	32.0	39.0
11	36.67764982333071	38.0	35.0	39.0	32.0	39.0
12	36.5999005271076	38.0	35.0	39.0	32.0	39.0
13	36.6878933097692	38.0	35.0	39.0	32.0	39.0
14	37.70765953613051	39.0	37.0	40.0	33.0	41.0
15	37.71782403664947	39.0	37.0	40.0	33.0	41.0
16	37.71702553836926	39.0	37.0	40.0	33.0	41.0
17	37.687743977015096	39.0	37.0	40.0	32.0	41.0
18	37.726786470205646	39.0	37.0	40.0	33.0	41.0
19	37.754292082525474	39.0	37.0	40.0	32.0	41.0
20	37.756413594957735	39.0	37.0	40.0	32.0	41.0
21	37.70918248339136	39.0	37.0	40.0	32.0	41.0
22	37.61717376038387	39.0	37.0	40.0	32.0	41.0
23	37.60220963109873	39.0	37.0	40.0	32.0	41.0
24	37.551509309848186	39.0	37.0	40.0	32.0	41.0
25	37.52005193324209	39.0	37.0	40.0	32.0	41.0
26	37.39396202011426	39.0	36.0	40.0	32.0	41.0
27	37.29949239205155	39.0	36.0	40.0	32.0	41.0
28	37.28237959892431	39.0	36.0	40.0	32.0	41.0
29	37.202281705750295	39.0	36.0	40.0	31.0	41.0
30	37.10958185594696	39.0	36.0	40.0	31.0	41.0
31	37.10974106194101	39.0	36.0	40.0	31.0	41.0
32	36.96834022197512	39.0	36.0	40.0	31.0	41.0
33	36.94533557291326	39.0	36.0	40.0	31.0	41.0
34	36.863011264132616	39.0	36.0	40.0	30.0	41.0
35	36.80025324860448	39.0	36.0	40.0	30.0	41.0
36	36.81108912943941	39.0	36.0	40.0	30.0	41.0
37	36.737806240134475	39.0	35.0	40.0	30.0	41.0
38	36.68991732395705	39.0	35.0	40.0	30.0	41.0
39	36.63300673478379	39.0	35.0	40.0	30.0	41.0
40	36.550941598551596	39.0	35.0	40.0	30.0	41.0
41	36.49732373489857	38.0	35.0	40.0	30.0	41.0
42	36.453041019609486	38.0	35.0	40.0	30.0	41.0
43	35.70684005721542	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	7.0
11	9.0
12	16.0
13	9.0
14	4.0
15	6.0
16	12.0
17	14.0
18	34.0
19	50.0
20	139.0
21	262.0
22	519.0
23	932.0
24	1650.0
25	2607.0
26	4090.0
27	6271.0
28	8959.0
29	12588.0
30	17162.0
31	22680.0
32	29381.0
33	38148.0
34	49710.0
35	66497.0
36	92108.0
37	136274.0
38	202318.0
39	117807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.94088644416498	22.88370187258337	15.634522277114693	23.54088940613696
2	14.46516042163671	23.03476244367625	39.99390327433661	22.506173860350426
3	15.556523681583077	28.636591955037265	34.01911212421523	21.787772239164426
4	12.147664176553278	19.511990432830498	38.1695753642917	30.17077002632453
5	11.975869801584901	39.137646540478435	35.874787571071906	13.011696086864767
6	27.056626733524958	40.74784855930917	17.92622468285302	14.269300024312853
7	24.160311796917327	33.67712777576885	23.119548891667108	19.043011535646713
8	24.567582944471663	35.264127680738916	22.538755552154775	17.62953382263465
9	24.349384341781946	15.309322436567518	19.687117026278862	40.65417619537167
10	14.347422035343731	28.97388651451181	34.177330794265124	22.501360655879328
11	31.003084153326483	23.857943823733045	25.138626459542547	20.000345563397925
12	20.413540654916687	27.033301204165028	29.97256473451475	22.580593406403537
13	28.69706554967412	21.49663507641271	26.98171352547481	22.82458584843836
14	21.619063251677524	22.555540060053982	27.379111433088436	28.446285255180058
15	23.51941510926591	29.292545333598262	23.496583241903018	23.691456315232806
16	23.597907366794566	28.223150032519982	24.108353871729335	24.070588728956118
17	20.835374831383575	30.516580255247938	25.618219089662592	23.029825823705895
18	22.992677758428968	27.175352443812006	26.447200998184556	23.384768799574463
19	23.63394469257816	28.391982435506147	26.72587319551113	21.24819967640456
20	22.921590430855847	26.189386020232735	26.852991159747784	24.036032389163626
21	23.90410122045587	26.73562301995258	26.931360001777183	22.42891575781436
22	22.017078236787448	29.933812267747456	26.421036912341673	21.628072583123426
23	22.670686720862527	27.372693827126977	27.142647336508404	22.8139721155021
24	23.51867461627036	28.57167540242709	26.364265782682583	21.545384198619967
25	21.85108439028424	27.752443318346575	26.56790135645975	23.828570934909433
26	23.96099576561422	28.196739115678582	26.993437997904408	20.84882712080279
27	22.091621198339816	28.87552436160248	26.011420870301414	23.02143356975629
28	21.3015151720844	27.3675103761581	28.84590464178034	22.48506980997716
29	23.55076264607767	27.89881410046762	26.501997479855504	22.048425773599202
30	21.164153721409257	28.07603875740339	27.67111250433497	23.088695016852387
31	24.237199652955617	26.16211119489652	27.719121133546675	21.881568018601183
32	23.017484273780006	27.03490560565539	27.18966864172604	22.75794147883856
33	22.54776488360067	27.552633625046436	28.598456565766266	21.301144925586623
34	22.917517719380307	27.85660599972108	26.989982363925158	22.235893916973456
35	21.512308844818588	26.40807828491949	29.89900909695645	22.180603773305474
36	23.962846998103103	26.463121597588955	26.302928279550912	23.271103124757026
37	22.389669629050037	27.152397160949853	28.154654430431304	22.30327877956881
38	22.206274197151323	25.98698460144816	28.418516767846807	23.388224433553713
39	23.56730032297836	26.593571780305602	27.03502902115465	22.804098875561387
40	21.082699491898392	26.22135063454079	30.74823114735687	21.94771872620395
41	22.217011345586847	26.75351826734512	27.026883598203565	24.002586788864466
42	22.475196570036445	25.781497795182105	29.268355895743525	22.47494973903793
43	22.612928267209366	25.223906569530442	29.34771206176699	22.815453101493205
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	17.5
2	17.0
3	84.0
4	151.0
5	151.0
6	202.0
7	253.0
8	278.0
9	303.0
10	453.0
11	603.0
12	603.0
13	931.0
14	1259.0
15	2988.0
16	4717.0
17	5190.0
18	5663.0
19	5663.0
20	6134.0
21	6605.0
22	8261.0
23	9917.0
24	12562.5
25	15208.0
26	15208.0
27	19082.0
28	22956.0
29	27326.0
30	31696.0
31	35486.0
32	39276.0
33	39276.0
34	42491.5
35	45707.0
36	48128.0
37	50549.0
38	53432.5
39	56316.0
40	56316.0
41	56319.5
42	56323.0
43	55561.0
44	54799.0
45	54637.0
46	54475.0
47	54475.0
48	53236.0
49	51997.0
50	52991.0
51	53985.0
52	63174.5
53	72364.0
54	72364.0
55	58232.5
56	44101.0
57	46549.0
58	48997.0
59	40327.0
60	31657.0
61	31657.0
62	25213.5
63	18770.0
64	15547.0
65	12324.0
66	10135.5
67	7947.0
68	7947.0
69	6252.0
70	4557.0
71	3872.5
72	3188.0
73	2546.0
74	1904.0
75	1904.0
76	1394.0
77	884.0
78	677.5
79	471.0
80	324.0
81	177.0
82	177.0
83	122.0
84	67.0
85	58.5
86	50.0
87	33.5
88	17.0
89	17.0
90	10.0
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	810271.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.574724285718226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.23679091541032	42.98663950863929
2	11.765399699711654	12.606560900466476
3	3.3563574715700493	5.394477784310272
4	1.3651502979293184	2.9255020328051726
5	0.7130784069355438	1.9101489522835469
6	0.46009393860012526	1.4789643543619129
7	0.3147677373255457	1.180451631887884
8	0.23175968879196648	0.9933169142058771
9	0.17975996446362108	0.8667531480374104
>10	1.1833303949404657	12.39472923880082
>50	0.12224246597789849	4.4881067444505724
>100	0.06688678097053452	6.0305602473994115
>500	0.0020757966503962013	0.8088690962736635
>1k	0.0013838644335974675	1.6672885297923226
>5k	6.919322167987337E-4	2.953373093497836
>10k+	2.3064407226624457E-4	1.3142578227874557
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	10636	1.3126472501175532	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	9943	1.2271203091311425	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	7971	0.9837449445926115	TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	5987	0.738888594062974	No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	2859	0.35284491238116633	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	2712	0.33470283399010947	Illumina Paired End PCR Primer 2 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2632	0.3248295940493983	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2534	0.3127348751220271	No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG	1415	0.17463293145132924	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	1341	0.16550018450617138	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	906	0.11181444232855427	No Hit
GGGATATACAAAGTGGAAATAGACACCAAATCTTACTGGAAGG	900	0.11107394933300094	No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA	853	0.10527342086783312	No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT	814	0.1004602163967364	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.2341549925888994E-4	0.0
6	0.0	0.0	0.0	1.2341549925888994E-4	0.0
7	0.0	0.0	0.0	1.2341549925888994E-4	0.0
8	0.0	0.0	0.0	1.2341549925888994E-4	0.0
9	0.0	0.0	0.0	1.2341549925888994E-4	0.0
10	0.0	0.0	0.0	2.468309985177799E-4	0.0
11	0.0	0.0	0.0	2.468309985177799E-4	0.0
12	0.0	0.0	0.0	2.468309985177799E-4	0.0
13	0.0	0.0	0.0	2.468309985177799E-4	0.0
14	0.0	0.0	0.0	2.468309985177799E-4	0.0
15	0.0	0.0	0.0	2.468309985177799E-4	0.0
16	0.0	0.0	0.0	2.468309985177799E-4	0.0
17	0.0	0.0	0.0	2.468309985177799E-4	0.0
18	0.0	0.0	0.0	3.702464977766698E-4	0.0
19	0.0	0.0	0.0	3.702464977766698E-4	0.0
20	0.0	0.0	0.0	3.702464977766698E-4	0.0
21	0.0	0.0	0.0	7.404929955533396E-4	0.0
22	0.0	0.0	0.0	0.0014809859911066791	0.0
23	0.0	0.0	0.0	0.001604401490365569	0.0
24	0.0	0.0	0.0	0.001851232488883349	0.0
25	0.0	0.0	0.0	0.001974647988142239	0.0
26	0.0	0.0	0.0	0.002098063487401129	0.0
27	0.0	0.0	0.0	0.003332218479990028	0.0
28	0.0	0.0	0.0	0.008145422951086735	0.0
29	0.0	0.0	0.0	0.01826549389031571	0.0
30	0.0	0.0	0.0	0.03282852280286472	0.0
31	0.0	0.0	0.0	0.07725810253606509	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	20	0.0018416155	37.0	2
GATTACG	65	8.731149E-11	31.307692	1
CTTATAC	1355	0.0	30.719559	1
GGTATCA	390	0.0	28.46154	1
TTATACA	1520	0.0	27.62829	2
TATACAC	1565	0.0	27.424921	3
CGTCTTC	385	0.0	27.38961	37
CCGTCTT	1235	0.0	25.615385	37
GTATCAA	1360	0.0	25.4375	1
GCCGTCT	1280	0.0	24.714844	36
GCAACGC	60	1.3365934E-6	24.666668	3
TCGCCGA	50	2.7011495E-4	22.199999	1
AGCAACG	70	5.0988565E-6	21.142859	2
TTACGGG	90	9.469659E-8	20.555555	3
TGCCGTC	1555	0.0	20.344053	35
ATGCCGT	1635	0.0	19.348625	34
CGGTACT	115	3.0468073E-9	19.304346	4
ATTACGG	110	3.8473445E-8	18.5	2
CGCCGAG	60	9.2336064E-4	18.5	2
TCTATAC	130	6.9485395E-10	18.5	3
>>END_MODULE