FastQCFastQC Report
Fri 10 Feb 2017
ERR1630922.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630922.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648619
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT119131.836671451190915No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG117991.819095647830236No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT108871.6784892209448072TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC60940.9395346112278549No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC49190.7583804976419131RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC32180.49613101065494536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18210.28075033262978727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17130.26409957155124963No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG13160.20289260721625485No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA11890.18331254557760412No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT9490.14631085429196491No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT8290.12781000864914535No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT7430.11455106927179129No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6920.10668820987359297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6720.1036047355997897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC13650.033.205131
TTATACA14700.030.9591852
GATTACG250.00549459929.61
CGTCTTC7350.029.19727937
TATACAC16300.028.0337413
GGTATCA3850.023.5454541
CGAGCAA400.001930284823.12500210
CCGTCTT19050.023.1128637
GCCGTCT19300.022.81347336
GTATCAA8950.021.2905031
GGACCGT705.096268E-621.1428596
ATGAGCG555.139775E-420.18181829
TGCCGTC23350.018.93575935
ATGCGTT801.614901E-518.536
TGCGTTA801.614901E-518.537
ATGCCGT24350.018.23408734
CGAATTA1258.558345E-917.76000215
CTATACA1156.392111E-817.6956521
TCTAATA1054.78929E-717.619052
TAGGACC852.7201535E-517.4117644
Produced by FastQC (version 0.11.5)