##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630922.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	648619
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.012865796407443	31.0	31.0	34.0	28.0	34.0
2	31.012989135378398	31.0	31.0	34.0	27.0	34.0
3	30.43485466814879	31.0	30.0	34.0	26.0	34.0
4	34.674354281943636	35.0	35.0	37.0	32.0	37.0
5	34.786346067568175	35.0	35.0	37.0	32.0	37.0
6	35.008986785771	36.0	35.0	37.0	32.0	37.0
7	35.01563475630532	36.0	35.0	37.0	32.0	37.0
8	35.04766126185018	36.0	35.0	37.0	32.0	37.0
9	36.76817823714692	38.0	37.0	39.0	33.0	39.0
10	36.54618659027873	38.0	35.0	39.0	32.0	39.0
11	36.62799578797414	38.0	35.0	39.0	32.0	39.0
12	36.54680482687063	38.0	35.0	39.0	32.0	39.0
13	36.646939112175254	38.0	35.0	39.0	32.0	39.0
14	37.6365462621354	39.0	37.0	40.0	32.0	41.0
15	37.644056063729245	39.0	37.0	40.0	32.0	41.0
16	37.631148640419106	39.0	37.0	40.0	32.0	41.0
17	37.60216706571963	39.0	37.0	40.0	32.0	41.0
18	37.65418681845583	39.0	37.0	40.0	32.0	41.0
19	37.66404468570918	39.0	37.0	40.0	32.0	41.0
20	37.655124194635064	39.0	37.0	40.0	32.0	41.0
21	37.573472254127616	39.0	37.0	40.0	32.0	41.0
22	37.49382457189814	39.0	36.0	40.0	32.0	41.0
23	37.49355785137346	39.0	37.0	40.0	32.0	41.0
24	37.4537686993443	39.0	36.0	40.0	32.0	41.0
25	37.41262898558322	39.0	36.0	40.0	32.0	41.0
26	37.23770965697891	39.0	36.0	40.0	31.0	41.0
27	37.14382557402728	39.0	36.0	40.0	31.0	41.0
28	37.08555561893808	39.0	36.0	40.0	31.0	41.0
29	37.02390309257052	39.0	36.0	40.0	31.0	41.0
30	36.936633061936206	39.0	36.0	40.0	31.0	41.0
31	36.89532221535293	39.0	36.0	40.0	31.0	41.0
32	36.774571821053655	39.0	36.0	40.0	30.0	41.0
33	36.719104744079345	39.0	36.0	40.0	30.0	41.0
34	36.631740667479676	39.0	35.0	40.0	30.0	41.0
35	36.6172190453872	39.0	35.0	40.0	30.0	41.0
36	36.62369125788791	39.0	35.0	40.0	30.0	41.0
37	36.524011785038674	38.0	35.0	40.0	30.0	41.0
38	36.48035441453303	38.0	35.0	40.0	30.0	41.0
39	36.394846589446196	38.0	35.0	40.0	30.0	41.0
40	36.259196847455904	38.0	35.0	40.0	30.0	41.0
41	36.21576919578366	38.0	35.0	40.0	29.0	41.0
42	36.15578637073536	38.0	35.0	40.0	29.0	41.0
43	35.310473482892114	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	4.0
10	12.0
11	17.0
12	12.0
13	11.0
14	7.0
15	12.0
16	11.0
17	19.0
18	36.0
19	67.0
20	136.0
21	252.0
22	504.0
23	838.0
24	1531.0
25	2530.0
26	3828.0
27	5586.0
28	7907.0
29	11091.0
30	14634.0
31	18989.0
32	24474.0
33	31437.0
34	40880.0
35	53943.0
36	75699.0
37	109515.0
38	159549.0
39	85082.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.68039326630888	23.0710170377371	15.907489604837355	24.341100091116665
2	14.016240659000124	22.421020660819373	40.415714001594154	23.14702467858635
3	15.119661927880621	28.507798877307017	34.80116987013948	21.571369324672883
4	11.997181704513745	19.608892123110795	36.931850593337536	31.462075579037922
5	12.413913252618256	38.6502708061281	35.4795342103762	13.456281730877448
6	27.040681817831423	40.11307100162036	17.78362952673295	15.062617653815261
7	23.885670940875922	34.17661215598063	22.361509607334966	19.57620729580848
8	24.757677465507484	34.86746456702625	22.662456696458165	17.712401271008098
9	23.382293765677538	14.989539313526123	19.268168215855532	42.35999870494081
10	14.219904134784828	28.6710688401049	33.74276732565651	23.366259699453764
11	30.213731019288677	23.111873071864995	25.244712227054713	21.429683681791623
12	19.44515963917184	26.99766735171187	28.79594954819393	24.76122346092236
13	28.778990439688013	20.508958263633968	27.12640240264315	23.585648894034865
14	21.628721946165623	23.197285309249345	26.312365194359096	28.861627550225943
15	24.198951927094335	28.999613023978636	22.54250954720722	24.25892550171981
16	24.773403184303884	27.81278377599176	23.313378115658036	24.100434924046322
17	20.46640631865548	31.356312411446474	24.741489225570017	23.43579204432803
18	23.05020358638893	25.878366190321284	25.60116185310637	25.47026837018342
19	23.87426208606285	27.21674819886559	25.756723130219743	23.152266584851816
20	23.761715275069033	26.49999460392002	25.636621807255107	24.10166831375584
21	24.339866701407143	27.345174902369497	24.920022386023227	23.394936010200134
22	23.151341542569675	30.37098820725264	25.3216449101861	21.156025339991583
23	24.16534205750988	25.78277848783338	25.91336362332895	24.13851583132779
24	23.261884095285524	27.511374165727492	25.953294615174705	23.273447123812286
25	23.592586711150922	27.276875947204754	27.16032061965499	21.97021672198933
26	24.559872590843007	27.293064187142217	25.229140681972005	22.917922540042767
27	23.776670125296977	28.966619849248943	26.081258797537537	21.175451227916543
28	21.64552688095785	27.458030060790694	27.47236821616388	23.424074842087574
29	23.034786215019913	25.89470860397244	25.99831333957223	25.07219184143542
30	24.67812382924336	25.942810802643773	28.545879784588486	20.83318558352438
31	23.238758038231996	27.162479051646653	25.311315271368862	24.28744763875249
32	24.068366791598766	26.164358429216534	28.986816605742355	20.780458173442344
33	24.318899076345282	25.66067290659077	28.82277577437602	21.197652242687926
34	21.036386538168014	28.400956493719733	26.613466457195983	23.94919051091627
35	21.31590348108828	25.700295551009145	32.76052659573648	20.223274372166095
36	24.80978818073476	25.761194167916756	25.389635517923466	24.039382133425015
37	22.167404901799053	26.554417924852647	28.05745745961805	23.220719713730247
38	22.27147215853991	24.921872470587513	28.437187316436923	24.369468054435657
39	24.256921243441838	25.739455674286443	26.83871425289731	23.164908829374408
40	20.584040862201075	25.081442264256832	32.12178489991813	22.21273197362396
41	22.0600999970707	26.27413011336393	26.666039693564326	24.999730196001043
42	22.61635875606481	24.898746413533985	30.36019604729433	22.12469878310688
43	22.566868993970264	24.49959066879015	29.882257534854823	23.05128280238476
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	12.0
2	16.0
3	67.5
4	119.0
5	119.0
6	157.0
7	195.0
8	204.0
9	213.0
10	331.0
11	449.0
12	449.0
13	671.0
14	893.0
15	2134.0
16	3375.0
17	3790.0
18	4205.0
19	4205.0
20	4756.0
21	5307.0
22	6703.0
23	8099.0
24	10097.5
25	12096.0
26	12096.0
27	14396.5
28	16697.0
29	19859.0
30	23021.0
31	25825.0
32	28629.0
33	28629.0
34	30753.0
35	32877.0
36	35428.5
37	37980.0
38	40216.5
39	42453.0
40	42453.0
41	43856.5
42	45260.0
43	44089.5
44	42919.0
45	43257.0
46	43595.0
47	43595.0
48	42748.5
49	41902.0
50	41808.5
51	41715.0
52	43040.5
53	44366.0
54	44366.0
55	51572.5
56	58779.0
57	43023.5
58	27268.0
59	32249.5
60	37231.0
61	37231.0
62	30805.5
63	24380.0
64	16991.5
65	9603.0
66	7816.0
67	6029.0
68	6029.0
69	4779.5
70	3530.0
71	3037.5
72	2545.0
73	1998.5
74	1452.0
75	1452.0
76	1097.0
77	742.0
78	576.0
79	410.0
80	270.5
81	131.0
82	131.0
83	101.0
84	71.0
85	55.0
86	39.0
87	27.5
88	16.0
89	16.0
90	9.5
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	648619.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.89404076536385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.02217852305819	39.615016996049945
2	11.080862264893462	10.835762625901658
3	3.015856036141214	4.423721639206715
4	1.251631509669069	2.4478928822789348
5	0.7432266118861991	1.8169676129733536
6	0.46403794214616956	1.361321403598222
7	0.3425487248645975	1.1724013922357124
8	0.25074962650825	0.9808129968315309
9	0.19768107252148256	0.8698883776565596
>10	1.3627395449447397	13.463201866441526
>50	0.16875493221823026	5.836770517421716
>100	0.09403417051133735	7.819358869202467
>500	0.002532906949800313	0.8628834577999711
>1k	0.001899680212350235	2.194516666267024
>5k	3.1661336872503915E-4	0.9433820939779377
>10k+	9.498401061751175E-4	5.356100602156657
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	11913	1.836671451190915	No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	11799	1.819095647830236	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	10887	1.6784892209448072	TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	6094	0.9395346112278549	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	4919	0.7583804976419131	RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	3218	0.49613101065494536	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1821	0.28075033262978727	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1713	0.26409957155124963	No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG	1316	0.20289260721625485	No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	1189	0.18331254557760412	No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT	949	0.14631085429196491	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	829	0.12781000864914535	No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT	743	0.11455106927179129	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	692	0.10668820987359297	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	672	0.1036047355997897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	1.5417371369016324E-4	0.0	0.0	0.0	0.0
11	1.5417371369016324E-4	0.0	0.0	0.0	0.0
12	1.5417371369016324E-4	0.0	0.0	1.5417371369016324E-4	0.0
13	1.5417371369016324E-4	0.0	0.0	1.5417371369016324E-4	0.0
14	1.5417371369016324E-4	0.0	0.0	1.5417371369016324E-4	0.0
15	1.5417371369016324E-4	0.0	0.0	1.5417371369016324E-4	0.0
16	1.5417371369016324E-4	0.0	0.0	1.5417371369016324E-4	0.0
17	1.5417371369016324E-4	0.0	0.0	1.5417371369016324E-4	0.0
18	1.5417371369016324E-4	0.0	0.0	1.5417371369016324E-4	0.0
19	1.5417371369016324E-4	0.0	0.0	1.5417371369016324E-4	0.0
20	3.083474273803265E-4	0.0	0.0	1.5417371369016324E-4	0.0
21	3.083474273803265E-4	0.0	0.0	6.16694854760653E-4	0.0
22	4.625211410704898E-4	0.0	0.0	0.001233389709521306	0.0
23	4.625211410704898E-4	0.0	0.0	0.001695910850591796	0.0
24	4.625211410704898E-4	0.0	0.0	0.0018500845642819592	0.0
25	4.625211410704898E-4	0.0	0.0	0.0018500845642819592	0.0
26	4.625211410704898E-4	0.0	0.0	0.0018500845642819592	0.0
27	6.16694854760653E-4	0.0	0.0	0.002929300560113102	0.0
28	6.16694854760653E-4	0.0	0.0	0.007708685684508163	0.0
29	6.16694854760653E-4	0.0	0.0	0.01942588792496057	0.0
30	6.16694854760653E-4	0.0	0.0	0.032376479874934284	0.0
31	6.16694854760653E-4	0.0	0.0	0.07045738715640461	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1365	0.0	33.20513	1
TTATACA	1470	0.0	30.959185	2
GATTACG	25	0.005494599	29.6	1
CGTCTTC	735	0.0	29.197279	37
TATACAC	1630	0.0	28.033741	3
GGTATCA	385	0.0	23.545454	1
CGAGCAA	40	0.0019302848	23.125002	10
CCGTCTT	1905	0.0	23.11286	37
GCCGTCT	1930	0.0	22.813473	36
GTATCAA	895	0.0	21.290503	1
GGACCGT	70	5.096268E-6	21.142859	6
ATGAGCG	55	5.139775E-4	20.181818	29
TGCCGTC	2335	0.0	18.935759	35
ATGCGTT	80	1.614901E-5	18.5	36
TGCGTTA	80	1.614901E-5	18.5	37
ATGCCGT	2435	0.0	18.234087	34
CGAATTA	125	8.558345E-9	17.760002	15
CTATACA	115	6.392111E-8	17.695652	1
TCTAATA	105	4.78929E-7	17.61905	2
TAGGACC	85	2.7201535E-5	17.411764	4
>>END_MODULE