FastQCFastQC Report
Fri 10 Feb 2017
ERR1630921.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630921.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706128
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG112591.5944701243967099No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT90931.287726871048875No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT72891.0322491106428295TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC63440.8984206829356717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29830.4224446559264043No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC29300.4149389345840981No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29170.4130979085944758No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC22600.3200552874266422RNA PCR Primer, Index 7 (95% over 22bp)
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG16580.23480162236874902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10570.14968957469467292No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10250.14515781841252579No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA10160.1438832619581719No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT8770.12419844560759521No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT7910.11201935059932477No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT7080.10026510774250562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12600.032.0079351
CGTCTTC3150.028.19047537
GATTACG405.936933E-527.7500021
TTATACA14800.027.6250022
GGTATCA4650.027.4516121
ACGGGCT556.256014E-726.9090916
TATACAC15550.026.6495193
CCGTCTT10550.026.30331837
GTATCAA16050.026.1651081
GCCGTCT10700.025.93457836
AGCAACG502.7006716E-422.22
GCAACGC502.7006716E-422.23
GTCGCCA1550.021.4838712
GGGACGA450.003824367720.5555576
TTAACGG450.003824367720.55555735
TCGCCAT1650.020.1818213
TTACGGG555.140396E-420.1818183
TGCCGTC14150.019.61130735
ATTACGG609.232009E-418.52
AGGACCG500.00703225918.55
Produced by FastQC (version 0.11.5)