##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630921.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	706128
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.540513901162395	31.0	30.0	33.0	27.0	34.0
2	30.512275394829267	31.0	30.0	34.0	27.0	34.0
3	29.90619547730723	31.0	30.0	34.0	25.0	34.0
4	34.20644557360705	35.0	35.0	37.0	30.0	37.0
5	34.12794989010491	35.0	33.0	37.0	30.0	37.0
6	34.40308272721093	35.0	35.0	37.0	30.0	37.0
7	34.460515657217954	35.0	35.0	37.0	30.0	37.0
8	34.502581684906986	35.0	35.0	37.0	30.0	37.0
9	36.14568038655881	37.0	35.0	39.0	32.0	39.0
10	35.83087485555027	37.0	35.0	39.0	30.0	39.0
11	35.978627104434324	37.0	35.0	39.0	31.0	39.0
12	35.86176727165613	37.0	35.0	39.0	30.0	39.0
13	35.99443018829447	37.0	35.0	39.0	31.0	39.0
14	36.850684295198604	38.0	36.0	40.0	31.0	41.0
15	36.84989831871842	38.0	36.0	40.0	31.0	41.0
16	36.86787806176784	38.0	36.0	40.0	31.0	41.0
17	36.80698541907416	38.0	36.0	40.0	31.0	41.0
18	36.90853358031405	38.0	36.0	40.0	31.0	41.0
19	36.9225409557474	39.0	36.0	40.0	31.0	41.0
20	36.93487582987787	39.0	36.0	40.0	31.0	41.0
21	36.88211485736297	38.0	36.0	40.0	31.0	41.0
22	36.700807785557295	38.0	36.0	40.0	31.0	41.0
23	36.748947782838236	38.0	36.0	40.0	31.0	41.0
24	36.675183536129424	38.0	35.0	40.0	31.0	41.0
25	36.63609572202207	38.0	35.0	40.0	30.0	41.0
26	36.488783903201686	38.0	35.0	40.0	30.0	41.0
27	36.354042043368906	38.0	35.0	40.0	30.0	41.0
28	36.36307864863028	38.0	35.0	40.0	30.0	41.0
29	36.25012887181927	38.0	35.0	40.0	30.0	41.0
30	36.17224355924139	38.0	35.0	40.0	30.0	41.0
31	36.14687563727823	38.0	35.0	40.0	30.0	41.0
32	36.01107306324066	38.0	35.0	40.0	30.0	41.0
33	35.953082160741396	38.0	34.0	40.0	30.0	41.0
34	35.8508400743208	38.0	34.0	40.0	29.0	41.0
35	35.811113565812434	38.0	34.0	40.0	29.0	41.0
36	35.80206987968187	38.0	34.0	40.0	29.0	41.0
37	35.71054681304239	38.0	34.0	40.0	29.0	41.0
38	35.66918037522942	38.0	34.0	40.0	28.0	41.0
39	35.5824156526862	38.0	34.0	40.0	28.0	41.0
40	35.460678517209345	38.0	34.0	40.0	27.0	41.0
41	35.40658350893889	38.0	34.0	40.0	27.0	41.0
42	35.3356062923436	38.0	34.0	40.0	27.0	41.0
43	34.527081492307346	37.0	33.0	40.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	14.0
11	10.0
12	8.0
13	11.0
14	5.0
15	11.0
16	20.0
17	30.0
18	41.0
19	77.0
20	239.0
21	403.0
22	703.0
23	1265.0
24	2042.0
25	3472.0
26	5236.0
27	7758.0
28	11124.0
29	15347.0
30	20840.0
31	27186.0
32	35022.0
33	45120.0
34	59005.0
35	77814.0
36	101809.0
37	125057.0
38	122716.0
39	43734.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.2406985702309	24.29474542859085	16.191257109192666	22.273298891985586
2	14.567897038497268	24.136842045634786	39.97079849545692	21.32446242041103
3	16.057003829334057	29.62196655563864	34.40934221557564	19.911687399451658
4	12.18815852083475	20.08970045090975	38.11844311512927	29.603697913126233
5	11.602853873518683	40.015266353975484	35.536191738608295	12.845688033897536
6	26.157014025785692	41.48567965014841	17.82509686629053	14.53220945777536
7	22.954761742913465	34.678840096979584	23.136881698502254	19.229516461604696
8	24.38368114562799	35.61648879523259	22.724491876826864	17.275338182312556
9	24.089116987288424	15.327249450524553	19.933213241791854	40.65042032039517
10	14.012048807015159	29.757919244103054	34.33088618494097	21.899145763940815
11	30.751081956812364	24.065891736342422	25.278419776584414	19.9046065302608
12	19.939869258831262	27.866902317993336	30.007024222237327	22.18620420093807
13	28.97704098973557	21.89263136428523	26.92047334194367	22.209854304035527
14	21.003982280832936	23.40113973670496	27.953147304737954	27.64173067772415
15	23.20485804273446	29.87659461174178	23.901049101579318	23.01749824394444
16	23.023304556680944	28.773820043958036	24.265572247524528	23.937303151836495
17	20.243213694967483	30.80064804114835	26.044711440418734	22.911426823465433
18	22.860727800058914	26.549435796342873	27.277490766546574	23.31234563705164
19	23.43965966510321	28.120963904561215	28.404057054811595	20.035319375523986
20	23.00631047062289	27.71480524777378	26.961117531099177	22.31776675050416
21	24.61437586386604	26.798399157093332	26.837768789794485	21.749456189246143
22	20.578705277230192	31.02355380327646	27.05005324813631	21.347687671357036
23	23.492058097115535	26.58979675073074	27.411602429021364	22.50654272313235
24	22.092453492851156	28.434504792332266	27.94068497496205	21.53235673985453
25	21.54552715654952	28.542983708336166	28.01772483175855	21.893764303355763
26	22.697159721750165	26.860994040740486	28.314838103006824	22.12700813450253
27	20.597115537126413	30.562872453719436	27.349149162758025	21.490862846396123
28	22.49195613259919	26.85306346724673	28.498090997666147	22.156889402487934
29	20.631386944010153	27.053310447964108	28.614075635012348	23.70122697301339
30	22.07602587632837	28.222078716606617	29.15236331090114	20.54953209616387
31	22.76187886615458	26.327521355901478	27.03178460562391	23.878815172320035
32	21.341881358620533	27.212205152606895	29.551157863730086	21.894755625042485
33	21.644942559989126	26.30061405297623	29.70467110778782	22.34977227924682
34	20.62855459633381	28.510694944825865	27.673028119547734	23.187722339292595
35	20.52319126277389	26.691478032311423	32.484053882582195	20.301276822332497
36	21.777213196474293	27.05515147395373	26.62449300976594	24.54314231980604
37	22.041612852060815	27.413301837627174	28.803418077175806	21.7416672331362
38	21.532073505086895	26.505817642127205	29.011029161851674	22.951079690934222
39	23.432862030679992	27.067755421113453	27.550953934697393	21.948428613509165
40	20.69893843609091	26.065529195839847	31.906113339224618	21.32941902884463
41	21.88285976480185	27.41443477669771	27.417691976525504	23.28501348197494
42	21.83499308907167	26.06623728275893	30.274822695035464	21.823946933133936
43	22.20447284345048	25.723098361770102	29.80762694582285	22.264801848956566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	24.0
2	30.0
3	101.0
4	172.0
5	172.0
6	235.5
7	299.0
8	311.0
9	323.0
10	506.0
11	689.0
12	689.0
13	1058.0
14	1427.0
15	3414.5
16	5402.0
17	5989.0
18	6576.0
19	6576.0
20	6861.5
21	7147.0
22	8753.0
23	10359.0
24	13107.5
25	15856.0
26	15856.0
27	19375.5
28	22895.0
29	27784.5
30	32674.0
31	34943.5
32	37213.0
33	37213.0
34	39386.0
35	41559.0
36	43183.5
37	44808.0
38	46414.0
39	48020.0
40	48020.0
41	49320.5
42	50621.0
43	48500.5
44	46380.0
45	46032.5
46	45685.0
47	45685.0
48	44306.0
49	42927.0
50	43312.0
51	43697.0
52	53245.0
53	62793.0
54	62793.0
55	48631.0
56	34469.0
57	37820.0
58	41171.0
59	33715.0
60	26259.0
61	26259.0
62	20206.5
63	14154.0
64	11625.0
65	9096.0
66	7365.5
67	5635.0
68	5635.0
69	4457.0
70	3279.0
71	2709.5
72	2140.0
73	1677.5
74	1215.0
75	1215.0
76	919.0
77	623.0
78	464.0
79	305.0
80	205.0
81	105.0
82	105.0
83	79.5
84	54.0
85	41.0
86	28.0
87	21.5
88	15.0
89	15.0
90	11.5
91	8.0
92	5.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	706128.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.15554202587126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.48600202957634	47.145321555475526
2	10.880521280498021	12.437641826217826
3	2.840923449549068	4.871235588389547
4	1.124414586791871	2.5706610067954183
5	0.6153406433839896	1.758506400158014
6	0.38129313860659214	1.307580960468326
7	0.2488074220570035	0.9954506147409427
8	0.17928862602801512	0.8197870879763947
9	0.14524079184051458	0.7471184563720216
>10	0.934076316476776	10.421175500335343
>50	0.10799417621814844	4.224111554160767
>100	0.05140215024909833	5.01338716007355
>500	0.0017298800564304509	0.6495905659812286
>1k	0.0019770057787776582	2.2381848463880294
>5k	7.413771670416217E-4	3.209957643507154
>10k+	2.471257223472073E-4	1.5902892329599159
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG	11259	1.5944701243967099	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	9093	1.287726871048875	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	7289	1.0322491106428295	TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC	6344	0.8984206829356717	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2983	0.4224446559264043	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	2930	0.4149389345840981	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2917	0.4130979085944758	No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC	2260	0.3200552874266422	RNA PCR Primer, Index 7 (95% over 22bp)
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG	1658	0.23480162236874902	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1057	0.14968957469467292	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1025	0.14515781841252579	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA	1016	0.1438832619581719	No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT	877	0.12419844560759521	No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT	791	0.11201935059932477	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	708	0.10026510774250562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	1.4161738381709833E-4	0.0
18	0.0	0.0	0.0	2.8323476763419665E-4	0.0
19	0.0	0.0	0.0	2.8323476763419665E-4	0.0
20	0.0	0.0	0.0	4.2485215145129495E-4	0.0
21	0.0	0.0	0.0	8.497043029025899E-4	0.0
22	0.0	0.0	0.0	0.001416173838170983	0.0
23	0.0	0.0	0.0	0.001841025989622278	0.0
24	0.0	0.0	0.0	0.0024074955248906712	0.0
25	0.0	0.0	0.0	0.0025491129087077697	0.0
26	0.0	0.0	0.0	0.002832347676341966	0.0
27	0.0	0.0	0.0	0.003115582443976163	0.0
28	0.0	0.0	0.0	0.006939251807037817	0.0
29	0.0	0.0	0.0	0.017277320825685994	0.0
30	0.0	0.0	0.0	0.032996850429383905	0.0
31	0.0	0.0	0.0	0.07534044819069631	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1260	0.0	32.007935	1
CGTCTTC	315	0.0	28.190475	37
GATTACG	40	5.936933E-5	27.750002	1
TTATACA	1480	0.0	27.625002	2
GGTATCA	465	0.0	27.451612	1
ACGGGCT	55	6.256014E-7	26.90909	16
TATACAC	1555	0.0	26.649519	3
CCGTCTT	1055	0.0	26.303318	37
GTATCAA	1605	0.0	26.165108	1
GCCGTCT	1070	0.0	25.934578	36
AGCAACG	50	2.7006716E-4	22.2	2
GCAACGC	50	2.7006716E-4	22.2	3
GTCGCCA	155	0.0	21.48387	12
GGGACGA	45	0.0038243677	20.555557	6
TTAACGG	45	0.0038243677	20.555557	35
TCGCCAT	165	0.0	20.18182	13
TTACGGG	55	5.140396E-4	20.181818	3
TGCCGTC	1415	0.0	19.611307	35
ATTACGG	60	9.232009E-4	18.5	2
AGGACCG	50	0.007032259	18.5	5
>>END_MODULE