FastQCFastQC Report
Fri 10 Feb 2017
ERR1630920.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630920.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054375
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT182651.7323058684054533No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT140011.3278956727919384RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG137291.3020983995257853No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC69720.6612448132780083No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC55060.5222050978067575RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC35140.3332780082987552RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19970.18940130409010078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19180.18190871369294606No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG16370.155257854179016No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCTTGTTCTTCTGGGCA15370.145773562537048No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA14140.1341078838174274No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT11710.11106105512744516No Hit
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCA11590.10992294013040901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC23050.032.5054241
TATACAC25950.029.514453
TTATACA26300.028.7699622
CGTCTTC8350.027.6946137
GATTACG706.5901986E-926.4285721
CCGTCTT23200.024.08189637
GCCGTCT23400.023.79700936
ATCGTAC701.9226172E-723.78571525
GTATCAA11750.023.144681
CGCCGAG400.001931081923.1252
GGTATCA6050.020.7933881
TTACGGG909.476389E-820.5555553
CGCCGTA450.003825602120.55555525
TGCCGTC27900.019.95878235
GTAGACC5800.019.137934
ATGCCGT29450.019.03395734
TACACAT41550.018.789415
ATACACA41100.018.5900234
AAAGCGG500.007034505318.537
ACGACAC801.6164639E-518.535
Produced by FastQC (version 0.11.5)