FastQCFastQC Report
Fri 10 Feb 2017
ERR1630919.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630919.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629585
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG82631.312451853204889No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT60640.9631741544032975RNA PCR Primer, Index 47 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT49960.7935386008243525No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC46620.7404877816339335No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC21050.33434722873003647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20820.33069402860614533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19930.3165577324745666No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC19560.31068084531874174RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG12130.19266659783825854No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT8420.1337388914920146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7650.12150861281637904No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA6720.10673697753281923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG200.001841303337.037
CTTATAC8250.030.0484871
GATTACG250.005494478629.61
GGTATCA4450.029.5168551
CGTCTTC3400.027.20588137
CCGTCTT10450.025.66985537
GCCGTCT10650.025.18779436
TTATACA10500.024.3142852
TATACAC10800.023.9814823
GATACAC952.8012437E-1023.3684223
GTCGCCC400.001930221823.12500237
GTATCAA12200.022.7459011
GCAACGC502.7002214E-422.23
TCTGACG450.003823915320.5555558
ACCGACC555.139542E-420.1818188
TGCCGTC13500.020.00740835
ATGCCGT14100.019.15602734
CGAGGGT609.230492E-418.516
TATGCCG15400.017.6590933
GCGAACT650.00157886117.07692328