Basic Statistics
Measure | Value |
---|---|
Filename | ERR1630919.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 629585 |
Sequences flagged as poor quality | 0 |
Sequence length | 43 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG | 8263 | 1.312451853204889 | No Hit |
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT | 6064 | 0.9631741544032975 | RNA PCR Primer, Index 47 (95% over 22bp) |
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT | 4996 | 0.7935386008243525 | No Hit |
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC | 4662 | 0.7404877816339335 | No Hit |
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC | 2105 | 0.33434722873003647 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT | 2082 | 0.33069402860614533 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1993 | 0.3165577324745666 | No Hit |
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC | 1956 | 0.31068084531874174 | RNA PCR Primer, Index 47 (95% over 23bp) |
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG | 1213 | 0.19266659783825854 | No Hit |
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT | 842 | 0.1337388914920146 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT | 765 | 0.12150861281637904 | No Hit |
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA | 672 | 0.10673697753281923 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGGATCG | 20 | 0.0018413033 | 37.0 | 37 |
CTTATAC | 825 | 0.0 | 30.048487 | 1 |
GATTACG | 25 | 0.0054944786 | 29.6 | 1 |
GGTATCA | 445 | 0.0 | 29.516855 | 1 |
CGTCTTC | 340 | 0.0 | 27.205881 | 37 |
CCGTCTT | 1045 | 0.0 | 25.669855 | 37 |
GCCGTCT | 1065 | 0.0 | 25.187794 | 36 |
TTATACA | 1050 | 0.0 | 24.314285 | 2 |
TATACAC | 1080 | 0.0 | 23.981482 | 3 |
GATACAC | 95 | 2.8012437E-10 | 23.368422 | 3 |
GTCGCCC | 40 | 0.0019302218 | 23.125002 | 37 |
GTATCAA | 1220 | 0.0 | 22.745901 | 1 |
GCAACGC | 50 | 2.7002214E-4 | 22.2 | 3 |
TCTGACG | 45 | 0.0038239153 | 20.555555 | 8 |
ACCGACC | 55 | 5.139542E-4 | 20.181818 | 8 |
TGCCGTC | 1350 | 0.0 | 20.007408 | 35 |
ATGCCGT | 1410 | 0.0 | 19.156027 | 34 |
CGAGGGT | 60 | 9.230492E-4 | 18.5 | 16 |
TATGCCG | 1540 | 0.0 | 17.65909 | 33 |
GCGAACT | 65 | 0.001578861 | 17.076923 | 28 |