##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630919.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	629585
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.422656194159647	31.0	30.0	33.0	27.0	34.0
2	30.385263308369797	31.0	30.0	33.0	27.0	34.0
3	29.805160542261966	31.0	30.0	34.0	25.0	34.0
4	34.087097055997205	35.0	33.0	37.0	28.0	37.0
5	33.853265246154216	35.0	33.0	37.0	28.0	37.0
6	34.13797978033149	35.0	33.0	37.0	30.0	37.0
7	34.21501306416131	35.0	35.0	37.0	30.0	37.0
8	34.265176266905975	35.0	35.0	37.0	30.0	37.0
9	35.86864363032792	37.0	35.0	39.0	30.0	39.0
10	35.52409920820858	37.0	35.0	39.0	30.0	39.0
11	35.71035046895971	37.0	35.0	39.0	30.0	39.0
12	35.596939253635334	37.0	35.0	39.0	30.0	39.0
13	35.75070244684991	37.0	35.0	39.0	30.0	39.0
14	36.59868961299904	38.0	36.0	40.0	31.0	41.0
15	36.596596170493264	38.0	36.0	40.0	31.0	41.0
16	36.60757483103949	38.0	36.0	40.0	31.0	41.0
17	36.51830332679464	38.0	35.0	40.0	31.0	41.0
18	36.61842483540745	38.0	36.0	40.0	31.0	41.0
19	36.627406942668586	38.0	35.0	40.0	31.0	41.0
20	36.63800439972363	38.0	35.0	40.0	31.0	41.0
21	36.57468014644567	38.0	35.0	40.0	30.0	41.0
22	36.41129474177434	38.0	35.0	40.0	30.0	41.0
23	36.439730933869136	38.0	35.0	40.0	30.0	41.0
24	36.359994281947635	38.0	35.0	40.0	30.0	41.0
25	36.31844151306019	38.0	35.0	40.0	30.0	41.0
26	36.13507151536329	38.0	35.0	40.0	30.0	41.0
27	36.01754647902984	38.0	34.0	40.0	29.0	41.0
28	35.98199448843285	38.0	34.0	40.0	29.0	41.0
29	35.862280708720824	38.0	34.0	40.0	29.0	41.0
30	35.783007854380266	38.0	34.0	40.0	29.0	41.0
31	35.77988039740464	38.0	34.0	40.0	29.0	41.0
32	35.63298204372722	38.0	34.0	40.0	28.0	41.0
33	35.59315580898529	38.0	34.0	40.0	28.0	41.0
34	35.46702192714248	38.0	34.0	40.0	27.0	41.0
35	35.41455244327613	38.0	34.0	40.0	27.0	41.0
36	35.44044569041512	38.0	34.0	40.0	27.0	41.0
37	35.34005575101059	38.0	34.0	40.0	27.0	41.0
38	35.28671108746238	38.0	34.0	40.0	27.0	41.0
39	35.23175742751177	38.0	34.0	40.0	27.0	41.0
40	35.11404655447636	38.0	33.0	40.0	27.0	41.0
41	35.06946004113821	38.0	33.0	40.0	27.0	41.0
42	35.00698873067179	38.0	33.0	40.0	26.0	41.0
43	34.18854483508978	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	3.0
11	11.0
12	6.0
13	9.0
14	4.0
15	7.0
16	18.0
17	36.0
18	74.0
19	131.0
20	271.0
21	462.0
22	842.0
23	1431.0
24	2321.0
25	3638.0
26	5632.0
27	8028.0
28	11406.0
29	15694.0
30	20684.0
31	26969.0
32	34278.0
33	43993.0
34	57158.0
35	73124.0
36	92372.0
37	105438.0
38	92384.0
39	33153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.30840950785041	23.674007481118515	15.78928977024548	22.228293240785597
2	15.618701207938562	23.658282837106984	38.79253794165998	21.930478013294472
3	16.989445428337717	29.18493928540229	32.70868905707729	21.116926229182713
4	12.730449423032633	19.553515410945305	38.23455133143261	29.48148383458945
5	12.084150670679893	39.47457452131166	35.472573202982915	12.968701605025531
6	27.280351342551047	40.819587506055576	17.69292470436875	14.207136447024627
7	24.41640128020839	33.88343114909028	23.267231589062636	18.4329359816387
8	24.80030496279295	35.31421491935164	22.336777400986364	17.548702716869048
9	24.660212679781125	15.166022062152052	19.99602913030012	40.17773612776671
10	14.30434333727773	29.14666010149543	34.1979240293209	22.35107253190594
11	31.463424319194388	23.98024095237339	24.598743616826958	19.957591111605264
12	20.660276213696324	27.181714939205985	30.149225283321552	22.008783563776138
13	29.07153124677367	21.699691066337348	26.43916230532811	22.789615381560868
14	21.795150773922504	22.593772087962705	27.474606288269253	28.136470849845534
15	23.413518428806277	29.776122366320674	23.592207565300953	23.2181516395721
16	23.313611347157252	28.415861241929207	24.419260306392307	23.85126710452123
17	20.795126948704304	30.223083459739353	25.962499106554315	23.019290485002024
18	22.843460374691265	27.06243001342154	26.750001985434853	23.344107626452345
19	23.814893938070316	28.53244597631773	27.1073802584242	20.545279827187752
20	23.318376390797113	26.702828053400257	27.550370482143	22.428425073659632
21	24.85446762549934	26.723476575839637	26.29398730910044	22.128068489560583
22	21.532755704154326	29.318360507318314	26.90200687754632	22.246876910981044
23	23.697038525377828	26.657083634457617	27.12151655455578	22.524361285608773
24	21.778155451607013	29.103139369584724	26.657877808397597	22.46082737041067
25	22.40364684673237	28.3021355337246	27.283210368734963	22.01100725080807
26	23.88494007957623	28.4495342169842	26.482841872026814	21.182683831412756
27	21.570081879333213	28.80437113336563	27.595479561933654	22.030067425367502
28	23.728328978612893	26.742536750399072	27.782110437828095	21.74702383315994
29	21.521796103782652	27.66536686865157	26.941397904969143	23.87143912259663
30	22.92827815148074	27.30306471723437	28.721618208820093	21.047038922464797
31	23.847613904397342	26.286680908852656	26.59291438010753	23.272790806642472
32	22.067393600546392	28.494484461986865	27.370251832556363	22.067870104910376
33	23.437820151369554	26.514291160049876	28.7651389407308	21.282749747849774
34	21.884098255199856	28.24130180992241	27.300523360626443	22.574076574251293
35	21.621862020219666	27.518762359331937	30.143348396165727	20.716027224282662
36	23.691638142585987	26.708069601404098	26.488877593970635	23.11141466203928
37	22.279755712096065	26.88389971171486	28.5067147406625	22.329629835526575
38	22.4060293685523	26.0805133540348	28.275927793705378	23.237529483707522
39	23.876521835812483	26.72442958456761	27.294487638682625	22.104560940937283
40	21.180301309592828	26.490783611426576	30.571090480236983	21.757824598743618
41	22.47718735357418	26.88453505086684	27.058300308933664	23.57997728662532
42	22.088836296925756	26.20503982782309	29.42986252849099	22.27626134676017
43	23.007854380266366	25.466299228857103	28.91555548496232	22.610290905914212
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15.0
1	18.5
2	22.0
3	74.0
4	126.0
5	126.0
6	181.0
7	236.0
8	225.0
9	214.0
10	352.0
11	490.0
12	490.0
13	758.5
14	1027.0
15	2223.5
16	3420.0
17	3944.5
18	4469.0
19	4469.0
20	4757.5
21	5046.0
22	6371.5
23	7697.0
24	9871.5
25	12046.0
26	12046.0
27	14822.5
28	17599.0
29	20672.5
30	23746.0
31	27395.5
32	31045.0
33	31045.0
34	33834.0
35	36623.0
36	38775.0
37	40927.0
38	42763.5
39	44600.0
40	44600.0
41	44748.0
42	44896.0
43	44968.0
44	45040.0
45	44327.5
46	43615.0
47	43615.0
48	42304.5
49	40994.0
50	40976.5
51	40959.0
52	46231.0
53	51503.0
54	51503.0
55	42374.5
56	33246.0
57	35097.0
58	36948.0
59	30616.5
60	24285.0
61	24285.0
62	19291.0
63	14297.0
64	11767.5
65	9238.0
66	7568.0
67	5898.0
68	5898.0
69	4762.5
70	3627.0
71	3096.5
72	2566.0
73	2041.0
74	1516.0
75	1516.0
76	1161.0
77	806.0
78	647.0
79	488.0
80	323.5
81	159.0
82	159.0
83	120.0
84	81.0
85	63.5
86	46.0
87	33.0
88	20.0
89	20.0
90	14.5
91	9.0
92	4.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	629585.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.22586566441106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.68513231452795	54.1751666515563
2	8.902797425748934	11.257741481557336
3	2.2192296313650584	4.209381436535029
4	0.9335425770845655	2.360961502830274
5	0.4600660975710676	1.4544038640889074
6	0.3287368923496201	1.2470804756779827
7	0.21506686767283184	0.9518452219043695
8	0.1555698047839211	0.7868828462965478
9	0.13347374737117265	0.7595093898913771
>10	0.8480466415117763	10.260983615958883
>50	0.08636158271742224	3.792430419992457
>100	0.02819967509544289	2.79477235272532
>500	0.0015106968801073847	0.6423391685588394
>1k	0.0017624796934586156	3.025759746418305
>5k	5.035656267024615E-4	2.2807418260080525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG	8263	1.312451853204889	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	6064	0.9631741544032975	RNA PCR Primer, Index 47 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	4996	0.7935386008243525	No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC	4662	0.7404877816339335	No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	2105	0.33434722873003647	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2082	0.33069402860614533	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1993	0.3165577324745666	No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC	1956	0.31068084531874174	RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG	1213	0.19266659783825854	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	842	0.1337388914920146	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	765	0.12150861281637904	No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA	672	0.10673697753281923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	1.5883478799526673E-4	0.0
16	0.0	0.0	0.0	1.5883478799526673E-4	0.0
17	0.0	0.0	0.0	1.5883478799526673E-4	0.0
18	0.0	0.0	0.0	1.5883478799526673E-4	0.0
19	0.0	0.0	0.0	1.5883478799526673E-4	0.0
20	0.0	0.0	0.0	4.7650436398580015E-4	0.0
21	0.0	0.0	0.0	6.353391519810669E-4	0.0
22	0.0	0.0	0.0	7.941739399763337E-4	0.0
23	0.0	0.0	0.0	9.530087279716003E-4	0.0
24	0.0	0.0	0.0	0.0014295130919574006	0.0
25	0.0	0.0	0.0	0.0014295130919574006	0.0
26	0.0	0.0	0.0	0.0014295130919574006	0.0
27	0.0	0.0	0.0	0.001747182667947934	0.0
28	0.0	0.0	0.0	0.0065122263078059356	0.0
29	0.0	0.0	0.0	0.013183287403607137	0.0
30	0.0	0.0	0.0	0.02366638341129474	0.0
31	0.0	0.0	0.0	0.057974697618272356	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATCG	20	0.0018413033	37.0	37
CTTATAC	825	0.0	30.048487	1
GATTACG	25	0.0054944786	29.6	1
GGTATCA	445	0.0	29.516855	1
CGTCTTC	340	0.0	27.205881	37
CCGTCTT	1045	0.0	25.669855	37
GCCGTCT	1065	0.0	25.187794	36
TTATACA	1050	0.0	24.314285	2
TATACAC	1080	0.0	23.981482	3
GATACAC	95	2.8012437E-10	23.368422	3
GTCGCCC	40	0.0019302218	23.125002	37
GTATCAA	1220	0.0	22.745901	1
GCAACGC	50	2.7002214E-4	22.2	3
TCTGACG	45	0.0038239153	20.555555	8
ACCGACC	55	5.139542E-4	20.181818	8
TGCCGTC	1350	0.0	20.007408	35
ATGCCGT	1410	0.0	19.156027	34
CGAGGGT	60	9.230492E-4	18.5	16
TATGCCG	1540	0.0	17.65909	33
GCGAACT	65	0.001578861	17.076923	28
>>END_MODULE