FastQCFastQC Report
Fri 10 Feb 2017
ERR1630918.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630918.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218080
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG132206.0619955979457085No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT112645.16507703595011TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC73253.358859134262656No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT52282.397285399853265No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC44042.019442406456346RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC37881.7369772560528247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24221.1106016140865738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20510.9404805575935437No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG16200.7428466617754952No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA10870.4984409391049156No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT10480.4805575935436537No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT9520.43653705062362436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9070.4159024211298606No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5160.23661041819515774No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4220.19350696991929567No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT3990.18296038151137198No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT3770.1728723404255319No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.14490095377842993No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3140.14398385913426265No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA2940.13481291269258988No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2690.1233492296404989No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2560.11738811445341159No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2550.11692956713132796No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2450.11234409391049156No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2450.11234409391049156No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2370.10867571533382245No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2190.10042186353631695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTATA200.001838667337.04
CGCCGAG251.2303477E-436.9999962
CTTATAC6750.035.0814821
CGTCTTC4700.032.27659637
AGCAACG352.3795064E-531.7142872
GCAACGC352.3795064E-531.7142873
TAGGACC352.3795064E-531.7142874
GGTATCA2600.031.3076921
TCGCCGA303.5900046E-430.8333321
AGGGATC303.5900046E-430.83333236
TTATACA7650.030.4705892
GACAGAC250.005486702529.59999816
CGCCTGT250.005486702529.5999981
ACGTCGC250.005486702529.59999816
AAAGGCT250.005486702529.5999985
CAAAGGC250.005486702529.5999984
TAGCAAC405.9184153E-527.7500021
CCGTCTT17600.027.01420437
TATACAC8950.026.871513
CTAATCC358.846268E-426.4285723
Produced by FastQC (version 0.11.5)