##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630918.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	218080
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.104140682318416	31.0	30.0	31.0	26.0	34.0
2	30.092154255319148	31.0	30.0	33.0	26.0	34.0
3	29.537853081438005	31.0	30.0	33.0	25.0	34.0
4	33.68622982391783	35.0	33.0	37.0	28.0	37.0
5	33.34175990462216	35.0	33.0	37.0	28.0	37.0
6	33.666773661041816	35.0	33.0	37.0	28.0	37.0
7	33.730741012472485	35.0	33.0	37.0	29.0	37.0
8	33.82296404988995	35.0	33.0	37.0	29.0	37.0
9	35.395276962582535	37.0	34.0	39.0	30.0	39.0
10	35.05702494497432	37.0	34.0	39.0	29.0	39.0
11	35.23843085106383	37.0	34.0	39.0	30.0	39.0
12	35.06733767424798	37.0	34.0	39.0	29.0	39.0
13	35.29267699926633	37.0	34.0	39.0	30.0	39.0
14	35.864925715333825	38.0	34.0	40.0	29.0	41.0
15	35.87361060161409	38.0	34.0	40.0	29.0	41.0
16	35.89380961115187	38.0	34.0	40.0	29.0	41.0
17	35.77897101980924	38.0	34.0	40.0	29.0	41.0
18	36.01253668378577	38.0	34.0	40.0	29.0	41.0
19	35.91432043286867	38.0	34.0	40.0	29.0	41.0
20	35.906570983125455	38.0	34.0	40.0	29.0	41.0
21	35.89299339691856	38.0	34.0	40.0	29.0	41.0
22	35.544639581804844	38.0	34.0	40.0	27.0	41.0
23	35.67495414526779	38.0	34.0	40.0	28.0	41.0
24	35.39809244314013	38.0	34.0	40.0	27.0	41.0
25	35.394763389581804	38.0	34.0	40.0	27.0	41.0
26	35.172569699192955	38.0	33.0	40.0	27.0	41.0
27	34.875252201027145	37.0	33.0	40.0	26.0	41.0
28	34.983373074101245	37.0	33.0	40.0	26.0	41.0
29	34.782162509170945	37.0	33.0	39.0	26.0	41.0
30	34.688843543653704	37.0	33.0	39.0	25.0	40.0
31	34.636862619222306	37.0	33.0	39.0	25.0	40.0
32	34.335280630961115	37.0	33.0	39.0	25.0	40.0
33	34.22249633162142	37.0	33.0	39.0	24.0	40.0
34	34.07708180484226	37.0	32.0	39.0	24.0	40.0
35	34.017644900953776	37.0	32.0	39.0	24.0	40.0
36	34.05855190755686	37.0	32.0	39.0	24.0	40.0
37	33.95017883345561	37.0	32.0	39.0	24.0	40.0
38	33.81540719002201	37.0	32.0	39.0	23.0	40.0
39	33.67471570066031	36.0	31.0	39.0	23.0	40.0
40	33.280465884079234	36.0	31.0	39.0	22.0	40.0
41	33.294268158473955	36.0	31.0	39.0	22.0	40.0
42	33.121139031548054	36.0	31.0	39.0	21.0	40.0
43	32.150559427732944	35.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	15.0
10	16.0
11	17.0
12	9.0
13	14.0
14	16.0
15	10.0
16	26.0
17	23.0
18	66.0
19	137.0
20	218.0
21	389.0
22	608.0
23	1067.0
24	1618.0
25	2403.0
26	3372.0
27	4717.0
28	6252.0
29	8141.0
30	10381.0
31	12727.0
32	15556.0
33	18223.0
34	21503.0
35	24163.0
36	25754.0
37	25122.0
38	23613.0
39	11900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.72230374174615	26.73055759354365	18.923330887747618	22.623807776962582
2	14.11775495231108	22.332171680117387	39.11546221570066	24.43461115187087
3	15.206346294937637	32.41287600880411	33.84675348495965	18.534024211298604
4	10.312270726338959	18.73119955979457	30.187545854732207	40.76898385913426
5	12.072175348495964	40.38609684519442	30.178374908290532	17.363352898019073
6	25.919387380777696	36.50953778429934	16.401779163609685	21.16929567131328
7	21.441214233308877	36.45772193690389	18.20157740278797	23.899486426999268
8	27.530722670579603	28.307043286867206	25.35812545854732	18.804108584005867
9	20.536500366837856	12.716892883345562	15.787784299339691	50.958822450476895
10	15.194424064563464	24.866104181951577	31.256878209831257	28.682593543653706
11	29.435069699192955	20.738719735876742	21.062454145267793	28.763756419662514
12	17.88930667644901	30.252201027146004	22.21891049156273	29.63958180484226
13	35.371881878209834	16.68745414526779	21.998807776962582	25.941856199559794
14	23.193782098312546	26.15049523110785	22.616471019809246	28.039251650770357
15	27.869589141599416	28.112619222303742	18.942131327953042	25.0756603081438
16	26.986885546588407	23.764214966984593	19.391049156272928	29.857850330154072
17	18.360234776228907	33.378576669112256	20.354457079970654	27.90673147468819
18	22.9736793837124	19.19341526045488	24.29200293470286	33.54090242112986
19	23.449192956713134	29.03750917094644	27.99156272927366	19.521735143066763
20	21.68103448275862	27.859042553191486	22.877842993396918	27.582079970652973
21	35.34299339691856	20.563096111518707	24.28191489361702	19.81199559794571
22	23.163517975055026	33.6546221570066	23.106658107116655	20.075201760821717
23	26.20185253118122	21.939655172413794	24.003118121790166	27.855374174614823
24	21.969002201027145	32.95854732208364	27.898019075568598	17.174431401320618
25	21.37472487160675	26.629218635363173	28.312545854732207	23.68351063829787
26	29.593727072633897	24.117296404988995	29.33235509904622	16.956621423330887
27	20.6754402054292	34.260821716801175	27.082721936903887	17.981016140865737
28	24.955979457079973	20.46038151137197	30.00641966250917	24.577219369038886
29	24.732208363903155	25.230649303008068	28.455612619222304	21.58152971386647
30	19.045763022743948	22.291819515774026	33.62206529713866	25.04035216434336
31	27.77787967718269	25.662600880410857	22.955337490829052	23.604181951577402
32	24.673055759354366	23.014948642699924	33.93112619222304	18.38086940572267
33	23.48312545854732	21.69249816581071	32.74532281731475	22.079053558327217
34	18.83207997065297	33.21946074834923	26.815847395451208	21.132611885546588
35	20.06052824651504	19.894534115920763	42.844369038884814	17.200568598679382
36	26.69203961848863	21.72230374174615	23.071349963316216	28.51430667644901
37	21.168378576669113	22.385821716801175	31.71588407923698	24.729915627292737
38	20.946900220102716	20.012380777696258	28.989820249449743	30.05089875275128
39	29.229640498899485	22.124908290535583	26.42149669845928	22.22395451210565
40	18.139214966984593	19.56804842259721	40.62270726338958	21.670029347028613
41	23.90728173147469	24.67993396918562	24.52494497432135	26.88783932501834
42	19.784024211298608	20.169203961848865	38.16351797505503	21.883253851797505
43	24.83079603815114	19.41168378576669	31.10097212032282	24.65654805575935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	7.5
2	7.0
3	49.0
4	91.0
5	91.0
6	138.5
7	186.0
8	197.5
9	209.0
10	326.0
11	443.0
12	443.0
13	694.0
14	945.0
15	2276.0
16	3607.0
17	3517.0
18	3427.0
19	3427.0
20	2868.0
21	2309.0
22	1892.5
23	1476.0
24	1524.0
25	1572.0
26	1572.0
27	1817.5
28	2063.0
29	2520.5
30	2978.0
31	3549.5
32	4121.0
33	4121.0
34	5131.5
35	6142.0
36	6828.0
37	7514.0
38	8530.0
39	9546.0
40	9546.0
41	10335.0
42	11124.0
43	11964.0
44	12804.0
45	13515.0
46	14226.0
47	14226.0
48	16226.0
49	18226.0
50	26138.5
51	34051.0
52	24390.0
53	14729.0
54	14729.0
55	23496.5
56	32264.0
57	25768.5
58	19273.0
59	12615.5
60	5958.0
61	5958.0
62	4978.0
63	3998.0
64	3034.0
65	2070.0
66	1691.5
67	1313.0
68	1313.0
69	1027.5
70	742.0
71	531.0
72	320.0
73	248.0
74	176.0
75	176.0
76	128.5
77	81.0
78	59.0
79	37.0
80	28.5
81	20.0
82	20.0
83	14.0
84	8.0
85	8.5
86	9.0
87	7.0
88	5.0
89	5.0
90	3.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	218080.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.22835656639765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.23976258037644	28.267149669845924
2	8.446359305443469	5.951027146001468
3	3.17991305026944	3.3606933235509904
4	1.8379194543513915	2.589875275128393
5	1.1532554083253064	2.031364636830521
6	0.7927003878895165	1.675531914893617
7	0.5883424882201338	1.450843727072634
8	0.4607815062609013	1.298606016140866
9	0.3878895165699112	1.22982391782832
>10	2.615000130164267	17.368396918561995
>50	0.21216775570770313	5.018800440205429
>100	0.06768541899877645	4.156272927366104
>500	0.0039049280191601806	1.0890498899486427
>1k	0.009111498711373754	7.529347028613353
>5k	0.0026032853461067868	5.756144534115921
>10k+	0.0026032853461067868	11.227072633895819
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	13220	6.0619955979457085	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	11264	5.16507703595011	TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	7325	3.358859134262656	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	5228	2.397285399853265	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	4404	2.019442406456346	RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	3788	1.7369772560528247	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2422	1.1106016140865738	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2051	0.9404805575935437	No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG	1620	0.7428466617754952	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	1087	0.4984409391049156	No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT	1048	0.4805575935436537	No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	952	0.43653705062362436	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	907	0.4159024211298606	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	516	0.23661041819515774	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	422	0.19350696991929567	No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT	399	0.18296038151137198	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	377	0.1728723404255319	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	316	0.14490095377842993	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	314	0.14398385913426265	No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	294	0.13481291269258988	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	269	0.1233492296404989	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	256	0.11738811445341159	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	255	0.11692956713132796	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	245	0.11234409391049156	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	245	0.11234409391049156	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	237	0.10867571533382245	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	219	0.10042186353631695	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	9.17094644167278E-4	0.0
23	0.0	0.0	0.0	0.001834189288334556	0.0
24	0.0	0.0	0.0	0.003209831254585473	0.0
25	0.0	0.0	0.0	0.003668378576669112	0.0
26	0.0	0.0	0.0	0.0045854732208363905	0.0
27	0.0	0.0	0.0	0.005502567865003668	0.0
28	0.0	0.0	0.0	0.011005135730007337	0.0
29	0.0	0.0	0.0	0.022010271460014674	0.0
30	0.0	0.0	0.0	0.03347395451210565	0.0
31	0.0	0.0	0.0	0.06006969919295671	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTATA	20	0.0018386673	37.0	4
CGCCGAG	25	1.2303477E-4	36.999996	2
CTTATAC	675	0.0	35.081482	1
CGTCTTC	470	0.0	32.276596	37
AGCAACG	35	2.3795064E-5	31.714287	2
GCAACGC	35	2.3795064E-5	31.714287	3
TAGGACC	35	2.3795064E-5	31.714287	4
GGTATCA	260	0.0	31.307692	1
TCGCCGA	30	3.5900046E-4	30.833332	1
AGGGATC	30	3.5900046E-4	30.833332	36
TTATACA	765	0.0	30.470589	2
GACAGAC	25	0.0054867025	29.599998	16
CGCCTGT	25	0.0054867025	29.599998	1
ACGTCGC	25	0.0054867025	29.599998	16
AAAGGCT	25	0.0054867025	29.599998	5
CAAAGGC	25	0.0054867025	29.599998	4
TAGCAAC	40	5.9184153E-5	27.750002	1
CCGTCTT	1760	0.0	27.014204	37
TATACAC	895	0.0	26.87151	3
CTAATCC	35	8.846268E-4	26.428572	3
>>END_MODULE