FastQCFastQC Report
Fri 10 Feb 2017
ERR1630917.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630917.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330851
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT149054.50504910065256No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG130843.9546502806399255No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT86622.6180969681216015Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC72202.182251224871619No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58451.7666562893870654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55061.6641932471112373No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC35861.0838715917437154No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC29900.9037300778900472Illumina PCR Primer Index 11 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22040.6661609002239679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21070.6368425665934212No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG16960.512617462241311No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15740.4757428570564997No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT13260.40078464323819485No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA12440.3760000725402069No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11140.33670746045803096No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11130.33640520959586034No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10420.3149453983817489No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT10200.308295879413996No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9890.29892610268670794No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9840.29741484837585497No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8880.2683987656074789No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8650.2614469957775555No Hit
ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT8070.24391644577166158No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG7180.21701611903847956No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6750.20401933196514443No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6720.2031125793786327No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6700.2025080776542915No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6690.20220582679212093No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6590.1991833181704151No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6440.19464955523785632No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6020.18195501902669178No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5880.1777235069563036No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5860.17711900523196242No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5830.17621225264545068No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5670.1713762388507213No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5610.1695627336776978No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5450.16472671988296847No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4860.14689391901490398No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4810.14538266470405106No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4700.14205790522017464No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4630.13994214918498055No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4600.1390353965984688No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4410.1332926302172277No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4300.12996787073335125No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4300.12996787073335125No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4240.12815436556032775No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4180.12634086038730427No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4060.12271385004125725No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3960.1196913414195514No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3940.11908683969521024No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3940.11908683969521024No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3850.11636658193567496No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3760.11364632417613971No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3620.10941481210575152No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3570.1079035577948986No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3540.10699680520838686No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3480.10518330003536334No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3470.10488104917319276No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3460.10457879831102218No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3380.10216079141365751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3350.10125403882714575No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA3330.10064953710280458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACAT355.6151475E-737.020
GTTATCG200.001840042237.011
CTTATAC18250.035.1753431
TTACGGG750.034.5333333
GATTACG750.034.5333331
CGTCTTC3600.034.43055737
ATTACTT651.8189894E-1234.1538472
ACTCAGA603.45608E-1133.9166681
TTATACA19150.033.4255872
TAGGACC507.2996045E-933.34
CTATACA451.0760232E-732.888891
CAGACAC401.5955102E-632.37518
TATACAC19800.032.328283
TTAGGAC601.2641976E-930.8333343
CCCCTAT303.593988E-430.8333341
AGTTTTT303.593988E-430.83333415
ACGTTTA303.593988E-430.83333426
ATCACGT303.593988E-430.83333423
TCACGTT303.593988E-430.83333424
TTACTTT751.4551915E-1129.63
Produced by FastQC (version 0.11.5)