FastQCFastQC Report
Fri 10 Feb 2017
ERR1630916.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630916.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309225
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT76672.4794243673700382RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG68742.2229767968307867No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT55021.7792869269949065No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC40511.3100493168404883No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC25550.8262591963780418RNA PCR Primer, Index 46 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC25230.815910744603444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.4414261460101867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12460.4029428409734012No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG10020.32403589619209316No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA6820.22055137844611528No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT5930.19176974694801519No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT5360.17333656722451288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5040.1629881154499151No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA4250.13744037513137683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA200.001839856537.01
CGTCTTC2650.035.60377537
CTTATAC6450.030.9767421
GAGATAA250.005490207629.624
TATACAC6950.029.0143893
CCGTCTT12850.028.93774237
GCCGTCT13150.028.27756736
GGTATCA1750.027.4857161
TTATACA7150.027.4265732
CTCCGTG358.8546774E-426.4285727
GTATCAA5400.025.6944431
TTAACGG601.3321296E-624.66666835
TCTAATA701.9134859E-723.7857152
CCGCCTT400.001928013723.12537
TGCCGTC16250.022.88307635
AACGGCC652.6723283E-622.7692337
ACGATAA603.714957E-521.58333417
ATGCCGT17050.021.48387134
GTTTAAC705.0820454E-621.14285933
TATGCCG18250.020.57808333
Produced by FastQC (version 0.11.5)