##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630916.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	309225
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.175069932896758	31.0	31.0	34.0	28.0	34.0
2	31.195624545234054	31.0	31.0	34.0	28.0	34.0
3	30.649944215377154	31.0	31.0	34.0	26.0	34.0
4	34.8289077532541	37.0	35.0	37.0	32.0	37.0
5	34.97687444417495	35.0	35.0	37.0	32.0	37.0
6	35.18318699975746	37.0	35.0	37.0	32.0	37.0
7	35.18517907672407	37.0	35.0	37.0	32.0	37.0
8	35.23130406661816	37.0	35.0	37.0	32.0	37.0
9	36.95312798124343	39.0	37.0	39.0	33.0	39.0
10	36.77482739105829	39.0	37.0	39.0	32.0	39.0
11	36.83089336243835	39.0	37.0	39.0	33.0	39.0
12	36.72326299620018	39.0	37.0	39.0	32.0	39.0
13	36.843065728838226	39.0	37.0	39.0	33.0	39.0
14	37.876918101705876	39.0	37.0	41.0	33.0	41.0
15	37.863060878001455	39.0	37.0	41.0	33.0	41.0
16	37.87251030802813	39.0	37.0	41.0	33.0	41.0
17	37.84514512086668	39.0	37.0	41.0	33.0	41.0
18	37.924705311666266	40.0	37.0	41.0	33.0	41.0
19	37.94018594874282	40.0	37.0	41.0	33.0	41.0
20	37.921519928854394	40.0	37.0	41.0	33.0	41.0
21	37.87622604899345	40.0	37.0	41.0	33.0	41.0
22	37.75592853100493	39.0	37.0	41.0	32.0	41.0
23	37.772948500282965	39.0	37.0	41.0	33.0	41.0
24	37.71812757700703	39.0	37.0	41.0	32.0	41.0
25	37.667336082140835	39.0	37.0	41.0	32.0	41.0
26	37.49149001536098	39.0	37.0	41.0	32.0	41.0
27	37.43195407874525	39.0	36.0	40.0	32.0	41.0
28	37.387730616864744	39.0	36.0	40.0	32.0	41.0
29	37.33485002829655	39.0	36.0	40.0	32.0	41.0
30	37.23557603686636	39.0	36.0	40.0	31.0	41.0
31	37.15564394858113	39.0	36.0	40.0	31.0	41.0
32	37.04488640957232	39.0	36.0	40.0	31.0	41.0
33	36.89931279812434	39.0	36.0	40.0	31.0	41.0
34	36.83243916242218	39.0	36.0	40.0	30.0	41.0
35	36.8231805319751	39.0	36.0	40.0	30.0	41.0
36	36.77514754628507	39.0	35.0	40.0	30.0	41.0
37	36.724989894090065	39.0	35.0	40.0	30.0	41.0
38	36.686946398253696	39.0	35.0	40.0	30.0	41.0
39	36.535965720753495	39.0	35.0	40.0	30.0	41.0
40	36.34352009054895	39.0	35.0	40.0	30.0	41.0
41	36.33069447813081	39.0	35.0	40.0	30.0	41.0
42	36.25741773789312	38.0	35.0	40.0	29.0	41.0
43	35.47882286361064	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	14.0
11	9.0
12	12.0
13	9.0
14	12.0
15	14.0
16	13.0
17	11.0
18	17.0
19	28.0
20	51.0
21	117.0
22	181.0
23	360.0
24	624.0
25	1028.0
26	1683.0
27	2308.0
28	3391.0
29	4969.0
30	6454.0
31	8487.0
32	10860.0
33	13992.0
34	18111.0
35	24139.0
36	33348.0
37	48567.0
38	76582.0
39	53831.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.25491147222896	24.162664726331958	16.65777346592287	23.92465033551621
2	13.573611447974777	22.189344328563344	40.17624706928612	24.060797154175763
3	14.521141563586385	29.03452178834182	34.80119653973644	21.643140108335356
4	11.270757539008812	19.196701430996846	36.36737003799822	33.16517099199612
5	12.10833535451532	38.36494461961355	35.119088042687366	14.407631983183766
6	27.330261136712753	39.436656156520336	17.5800792303339	15.653003476433017
7	23.403347077370846	34.41054248524537	21.921578138895626	20.264532298488156
8	25.524132912927477	33.82553157086264	22.741369552914545	17.908965963295334
9	22.7940819791414	15.089659632953351	18.93378607809847	43.182472309806776
10	14.534723906540545	28.159754224270355	32.9940981485973	24.3114237205918
11	30.531813404478942	22.806370765623736	24.224755436979546	22.43706039291778
12	19.493896030398577	27.725119249737247	27.99935322176409	24.78163149810009
13	29.659309564233165	20.107365187161452	25.585253456221196	24.648071792384187
14	21.926105586547013	23.17083030156035	26.029913493410945	28.873150618481684
15	24.23898455816962	29.481445549357264	21.979464791009782	24.300105101463334
16	25.533834586466163	26.972269383135256	22.816072439162422	24.677823591236155
17	20.19144635783006	32.06661815829897	24.03039857708788	23.71153690678309
18	23.05926105586547	24.90063869350796	26.07130730050934	25.96879295011723
19	22.942840973401246	28.30721966205837	27.978979707332847	20.770959657207534
20	21.503436009378284	28.998625596248683	25.045193629234376	24.452744765138654
21	24.922629153528984	27.33252486053844	25.690031530439	22.054814455493574
22	23.343196701430998	30.989409006386936	25.20333090791495	20.46406338426712
23	25.12959818902094	25.41741450400194	25.631821489206892	23.821165817770233
24	23.418222976796834	27.85447489691972	26.0981485972997	22.62915352898375
25	24.06952866036058	29.07462203896839	24.9313606597138	21.924488640957232
26	22.6449995957636	29.660926509822943	27.37262511116501	20.321448783248446
27	22.648880265179077	31.354191931441505	25.748241571671116	20.248686231708305
28	21.370199692780336	27.829573934837093	28.219904600210207	22.580321772172365
29	23.01430996846956	25.822297679683075	27.638774355242944	23.524617996604412
30	24.323065728838227	26.824480556229286	28.28037836526801	20.572075349664484
31	21.534804753820033	27.869997574581618	25.17519605465276	25.42000161694559
32	26.810251435039213	25.790282157005418	26.977443609022554	20.422022798932815
33	21.922224917131537	27.428894817689386	29.32524860538443	21.323631659794646
34	21.271566011803703	28.835314091680814	26.442234618805077	23.450885277710405
35	22.713234699652357	24.960142291211902	32.18012773870159	20.14649527043415
36	23.31473845905085	27.31829573934837	25.159996766108822	24.20696903549196
37	21.635378769504406	26.12822378526963	28.902255639097746	23.334141806128223
38	22.208100897404805	24.337618239146252	28.219581211092247	25.234699652356696
39	25.208181744684293	25.420971784299457	26.516937505052958	22.853908965963296
40	20.259034683482902	24.487347400759965	33.249575551782684	22.004042363974452
41	22.39016897081413	26.013097259277224	26.114964831433422	25.48176893847522
42	21.932896758024093	24.19403347077371	31.723502304147466	22.149567467054734
43	22.929582019565043	24.013258953836203	29.834263077047456	23.222895949551297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	10.0
2	11.0
3	64.0
4	117.0
5	117.0
6	153.0
7	189.0
8	170.5
9	152.0
10	222.5
11	293.0
12	293.0
13	467.5
14	642.0
15	1448.0
16	2254.0
17	2530.5
18	2807.0
19	2807.0
20	2822.5
21	2838.0
22	3288.5
23	3739.0
24	4609.5
25	5480.0
26	5480.0
27	6825.0
28	8170.0
29	9357.5
30	10545.0
31	11717.5
32	12890.0
33	12890.0
34	14149.0
35	15408.0
36	16317.5
37	17227.0
38	18144.0
39	19061.0
40	19061.0
41	19287.0
42	19513.0
43	19782.0
44	20051.0
45	19978.0
46	19905.0
47	19905.0
48	20239.5
49	20574.0
50	26070.0
51	31566.0
52	24753.0
53	17940.0
54	17940.0
55	22973.0
56	28006.0
57	23347.5
58	18689.0
59	14218.5
60	9748.0
61	9748.0
62	8613.5
63	7479.0
64	6320.5
65	5162.0
66	4335.0
67	3508.0
68	3508.0
69	2864.5
70	2221.0
71	1863.5
72	1506.0
73	1146.5
74	787.0
75	787.0
76	580.5
77	374.0
78	291.5
79	209.0
80	147.5
81	86.0
82	86.0
83	65.0
84	44.0
85	32.5
86	21.0
87	12.5
88	4.0
89	4.0
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	309225.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.27054680805045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.23506349693241	50.51928820682085
2	8.777070048449113	10.404434822882818
3	2.294377439474498	4.0796701626512455
4	1.0214083529744356	2.4215772638042
5	0.5610948293548541	1.6628198673515984
6	0.3819680906151589	1.358367455639246
7	0.2415833071866172	1.0023142301653618
8	0.19684878424507424	0.9333868064564399
9	0.15770020635625137	0.8412279716229671
>10	1.045759566609934	11.46178490312639
>50	0.0653444083512577	2.6433216904373245
>100	0.014702491879032982	1.3432850885958665
>500	0.002178146945041923	0.747166021167571
>1k	0.0032672204175628844	4.112479240482587
>5k	0.0016336102087814422	6.468876268795519
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	7667	2.4794243673700382	RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	6874	2.2229767968307867	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	5502	1.7792869269949065	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	4051	1.3100493168404883	No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC	2555	0.8262591963780418	RNA PCR Primer, Index 46 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	2523	0.815910744603444	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1365	0.4414261460101867	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1246	0.4029428409734012	No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG	1002	0.32403589619209316	No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	682	0.22055137844611528	No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	593	0.19176974694801519	No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT	536	0.17333656722451288	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	504	0.1629881154499151	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	425	0.13744037513137683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.233891179561808E-4	0.0
11	0.0	0.0	0.0	3.233891179561808E-4	0.0
12	0.0	0.0	0.0	6.467782359123616E-4	0.0
13	0.0	0.0	0.0	6.467782359123616E-4	0.0
14	0.0	0.0	0.0	6.467782359123616E-4	0.0
15	0.0	0.0	0.0	6.467782359123616E-4	0.0
16	0.0	0.0	0.0	0.0012935564718247231	0.0
17	0.0	0.0	0.0	0.0012935564718247231	0.0
18	0.0	0.0	0.0	0.0012935564718247231	0.0
19	0.0	0.0	0.0	0.0012935564718247231	0.0
20	0.0	0.0	0.0	0.0012935564718247231	0.0
21	0.0	0.0	0.0	0.0019403347077370846	0.0
22	0.0	0.0	0.0	0.002910502061605627	0.0
23	0.0	0.0	0.0	0.0035572802975179884	0.0
24	0.0	0.0	0.0	0.00420405853343035	0.0
25	0.0	0.0	0.0	0.00420405853343035	0.0
26	0.0	0.0	0.0	0.00420405853343035	0.0
27	0.0	0.0	0.0	0.005821004123211254	0.0
28	0.0	0.0	0.0	0.009378284420729242	0.0
29	0.0	0.0	0.0	0.01746301236963376	0.0
30	0.0	0.0	0.0	0.030721966205837174	0.0
31	0.0	0.0	0.0	0.06338426711941143	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAA	20	0.0018398565	37.0	1
CGTCTTC	265	0.0	35.603775	37
CTTATAC	645	0.0	30.976742	1
GAGATAA	25	0.0054902076	29.6	24
TATACAC	695	0.0	29.014389	3
CCGTCTT	1285	0.0	28.937742	37
GCCGTCT	1315	0.0	28.277567	36
GGTATCA	175	0.0	27.485716	1
TTATACA	715	0.0	27.426573	2
CTCCGTG	35	8.8546774E-4	26.428572	7
GTATCAA	540	0.0	25.694443	1
TTAACGG	60	1.3321296E-6	24.666668	35
TCTAATA	70	1.9134859E-7	23.785715	2
CCGCCTT	40	0.0019280137	23.125	37
TGCCGTC	1625	0.0	22.883076	35
AACGGCC	65	2.6723283E-6	22.76923	37
ACGATAA	60	3.714957E-5	21.583334	17
ATGCCGT	1705	0.0	21.483871	34
GTTTAAC	70	5.0820454E-6	21.142859	33
TATGCCG	1825	0.0	20.578083	33
>>END_MODULE