FastQCFastQC Report
Fri 10 Feb 2017
ERR1630915.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630915.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342452
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT147094.29520049525189No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG116933.4144931260439417No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT105433.0786796397743332RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC61901.8075525913120671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50851.4848796327660518No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC46331.3528903320757362RNA PCR Primer, Index 27 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44111.2880637286393422No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC30470.8897597327508673RNA PCR Primer, Index 27 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18800.5489820471190123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17550.5124805812201417No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG13660.39888801934285684No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA11610.3390256152687092No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10530.3074883487320851No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9810.28646350437433565No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT9310.27186291801478746No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8150.23798955766063562No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8100.23652949902468082No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7780.22718512375456998No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7730.22572506511861515No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT7540.22017684230198684No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7090.20703631457839347No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6910.20178010348895614No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6790.19827596276266451No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6650.19418779858199106No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.17958721222244284No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5090.1486339691402007No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5000.146005863595482No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4860.1419176994148085No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4710.13753752350694404No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4390.1281931482368332No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4360.1273171130552603No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4210.12293693714739584No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3980.11622066742200367No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3970.1159286556948127No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3950.11534463224043078No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3770.11008842115099342No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3740.10921238596942054No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA3690.10775232733346571No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3660.10687629215189283No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3640.10629226869751089No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.10074404588088258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGT200.001840132237.04
GCGTCAC200.001840132237.026
GATTACG603.45608E-1133.9166681
TTATACA18300.033.259562
TATACAC18750.032.658673
CTTATAC18700.032.647061
CGTCTTC5400.031.51851837
ATTACGG658.731149E-1131.3076922
AGGCGTC250.00549102429.59999824
GACTTGC250.00549102429.59999815
CTTAGGA604.3042746E-827.7500022
GACCGTT556.2401887E-726.909097
CTCTTAC706.557457E-926.4285731
AGCTAGT358.856633E-426.428576
TAGCTAG358.856633E-426.428575
CTGTGCA509.066778E-625.8999989
TAGGACC809.731593E-1025.43754
CTTACTT809.731593E-1025.437533
CCGTCTT16450.025.1914937
GTACATG601.3328299E-624.6666681
Produced by FastQC (version 0.11.5)