##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630915.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	342452
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.101263826755282	31.0	31.0	34.0	28.0	34.0
2	31.10445259481621	31.0	31.0	34.0	28.0	34.0
3	30.508024482263206	31.0	30.0	34.0	26.0	34.0
4	34.71132596685083	35.0	35.0	37.0	32.0	37.0
5	34.92892434560172	35.0	35.0	37.0	32.0	37.0
6	35.16741032319858	36.0	35.0	37.0	32.0	37.0
7	35.164104750446775	37.0	35.0	37.0	32.0	37.0
8	35.19642460841227	37.0	35.0	37.0	32.0	37.0
9	36.94366509759032	39.0	37.0	39.0	33.0	39.0
10	36.75044385782533	39.0	37.0	39.0	32.0	39.0
11	36.81846506955719	39.0	37.0	39.0	32.0	39.0
12	36.69482146402999	39.0	35.0	39.0	32.0	39.0
13	36.81093700723021	39.0	37.0	39.0	32.0	39.0
14	37.651729293448426	39.0	37.0	40.0	32.0	41.0
15	37.66618679406165	39.0	37.0	40.0	32.0	41.0
16	37.69428124233469	39.0	37.0	40.0	32.0	41.0
17	37.68131300153014	39.0	37.0	40.0	32.0	41.0
18	37.80874983939355	39.0	37.0	41.0	33.0	41.0
19	37.7648137549204	39.0	37.0	41.0	32.0	41.0
20	37.70010395617488	39.0	37.0	41.0	32.0	41.0
21	37.60401457722542	39.0	37.0	41.0	32.0	41.0
22	37.528935442047356	39.0	36.0	40.0	32.0	41.0
23	37.576889607886656	39.0	36.0	40.0	32.0	41.0
24	37.47484026958523	39.0	36.0	40.0	32.0	41.0
25	37.37075268942801	39.0	36.0	40.0	32.0	41.0
26	37.20804667515448	39.0	36.0	40.0	31.0	41.0
27	37.05109621202387	39.0	36.0	40.0	31.0	41.0
28	36.977591020055364	39.0	36.0	40.0	31.0	41.0
29	36.97849333629239	39.0	36.0	40.0	31.0	41.0
30	36.72785091049256	39.0	35.0	40.0	30.0	41.0
31	36.517024283695235	38.0	35.0	40.0	30.0	41.0
32	36.42172333640919	38.0	35.0	40.0	30.0	41.0
33	36.35954527933842	38.0	35.0	40.0	30.0	41.0
34	36.267640428439606	38.0	35.0	40.0	30.0	41.0
35	36.20401399320197	38.0	35.0	40.0	30.0	41.0
36	36.09742387254272	38.0	35.0	40.0	30.0	41.0
37	35.93205763143448	38.0	35.0	40.0	29.0	41.0
38	35.81829278263815	38.0	35.0	40.0	28.0	41.0
39	35.59913506126406	38.0	34.0	40.0	27.0	41.0
40	35.337031175171994	38.0	34.0	40.0	26.0	41.0
41	35.23810636235151	38.0	34.0	40.0	26.0	41.0
42	35.040177893544204	38.0	34.0	40.0	25.0	41.0
43	34.07180276359899	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	13.0
11	17.0
12	13.0
13	9.0
14	14.0
15	15.0
16	19.0
17	14.0
18	23.0
19	55.0
20	89.0
21	152.0
22	351.0
23	568.0
24	989.0
25	1597.0
26	2460.0
27	3748.0
28	5035.0
29	6828.0
30	8829.0
31	10924.0
32	13314.0
33	17109.0
34	21940.0
35	28211.0
36	38587.0
37	54899.0
38	76992.0
39	49625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.66411643091587	22.257717869949655	16.623643605527196	24.454522093607277
2	13.07949727261047	22.0398771214652	41.90076273463143	22.979862871292912
3	14.744548141053345	30.030194012591544	35.66280821837805	19.56244962797706
4	10.186829103056779	20.1978671463446	32.796421104271545	36.818882646327076
5	13.160092509315174	38.118042820599676	32.479588380269355	16.2422762898158
6	26.333909569808323	39.63066356744887	15.799294499667107	18.2361323630757
7	23.50431593332788	33.61639003422378	18.548584911170032	24.33070912127831
8	27.29638022262974	31.25489119643045	22.442561293261537	19.006167287678274
9	22.30969595738965	12.103886092065459	17.563921367082102	48.02249658346279
10	15.438952028313457	28.28688400126149	29.35593893450761	26.91822503591744
11	30.988868512959485	21.248525340777686	23.987303330101735	23.775302816161098
12	20.217723943793583	27.415521007323658	24.378307032810437	27.988448016072326
13	32.82766635908098	18.23262822234941	25.97152301636434	22.968182402205272
14	22.21362409914382	23.421676614532842	25.904068307383227	28.460630978940117
15	26.634389637087825	26.259154567647435	21.833132818613997	25.273322976650743
16	26.59058787800918	24.595855769567706	21.114783969724225	27.698772382698888
17	19.580262343335708	31.978496256409656	22.510892037424224	25.930349362830412
18	26.7450620816932	20.27174611332391	25.15213810986649	27.8310536951164
19	28.622697487531102	24.572494831392426	24.90276009484541	21.902047586231063
20	24.37217478653943	22.63149288075409	25.721560977888874	27.274771354817613
21	26.47144709331527	24.104984056159694	25.62724119000619	23.796327660518845
22	19.966301846682164	31.286136451239884	26.677607372712085	22.069954329365867
23	23.562718278766077	21.407963743823952	29.28118393234672	25.74813404506325
24	25.600960134559003	24.76639061824723	25.068330744162687	24.56431850303108
25	20.927312440867627	26.486339691402005	27.83251375375235	24.75383411397802
26	27.315652996624344	24.88173525048766	28.54940254400617	19.253209208881827
27	23.03680515809515	28.712637099505915	27.273311296181657	20.97724644621728
28	18.31088736523659	25.183967388130306	31.929730297968767	24.57541494866434
29	24.750621984978917	22.14295726116361	25.61147255673788	27.494948197119594
30	23.20091574877647	21.652377559482787	34.10112950136078	21.045577190379966
31	21.708151799376264	24.99445177718337	27.103068459229323	26.194327964211045
32	20.261525702872227	22.451613656804454	28.94186630535082	28.34499433497249
33	17.837828367187225	22.203695700419328	39.724399331877166	20.234076600516275
34	18.370165745856355	27.55656267155689	27.685047831520915	26.388223751065844
35	19.38578253302653	22.748881595084857	34.937159076308504	22.92817679558011
36	23.276254774391738	22.25800988167685	29.80184084192821	24.6638945020032
37	21.082370668006025	25.94787006646187	29.512457220281966	23.457302045250135
38	20.99856330230222	21.28298272458622	30.878780091808487	26.83967388130307
39	25.592199782743275	22.15551376543282	27.230677584011776	25.021608867812134
40	18.78657446883067	22.645217431932068	37.472988915234836	21.095219184002428
41	22.639669209115436	23.46664642052025	26.14760608786049	27.746078282503827
42	23.36648639809375	21.625512480581218	33.53754686788221	21.47045425344282
43	23.31071215820027	21.259913798138133	32.12450212000514	23.304871923656453
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	9.5
2	16.0
3	109.0
4	202.0
5	202.0
6	293.5
7	385.0
8	418.0
9	451.0
10	686.0
11	921.0
12	921.0
13	1391.0
14	1861.0
15	4689.5
16	7518.0
17	7389.0
18	7260.0
19	7260.0
20	6048.0
21	4836.0
22	3973.0
23	3110.0
24	3294.0
25	3478.0
26	3478.0
27	3848.0
28	4218.0
29	4700.5
30	5183.0
31	6083.5
32	6984.0
33	6984.0
34	8417.0
35	9850.0
36	10784.5
37	11719.0
38	12700.0
39	13681.0
40	13681.0
41	14290.0
42	14899.0
43	16554.0
44	18209.0
45	19677.5
46	21146.0
47	21146.0
48	23905.0
49	26664.0
50	27431.5
51	28199.0
52	40640.0
53	53081.0
54	53081.0
55	38796.0
56	24511.0
57	30067.0
58	35623.0
59	28169.0
60	20715.0
61	20715.0
62	14232.5
63	7750.0
64	5994.5
65	4239.0
66	3501.5
67	2764.0
68	2764.0
69	2127.0
70	1490.0
71	1160.0
72	830.0
73	567.0
74	304.0
75	304.0
76	231.5
77	159.0
78	122.0
79	85.0
80	72.0
81	59.0
82	59.0
83	38.0
84	17.0
85	18.0
86	19.0
87	13.0
88	7.0
89	7.0
90	6.0
91	5.0
92	3.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	342452.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.64194356776313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.99244384480676	22.229217884564072
2	12.210627851280547	7.238934833892295
3	4.103301204733785	3.6488946825675987
4	2.0134579128820635	2.3873122331886503
5	1.2056697905525413	1.7869197946457613
6	0.8107777666915361	1.441981728376052
7	0.6138317430844972	1.273661612202864
8	0.44687750655786324	1.0597054264872705
9	0.35790189562436886	0.9548017013603693
>10	2.658271342078603	16.01708006159854
>50	0.34590518403880843	7.214353637365687
>100	0.21494108256746766	11.859158686126
>500	0.012996437550591067	2.877443985164134
>1k	0.007997807723440657	5.72110541478669
>5k	0.0019994519308601643	3.341213278344553
>10k+	0.0029991778962902465	10.948215039329446
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	14709	4.29520049525189	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG	11693	3.4144931260439417	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	10543	3.0786796397743332	RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	6190	1.8075525913120671	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5085	1.4848796327660518	No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC	4633	1.3528903320757362	RNA PCR Primer, Index 27 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4411	1.2880637286393422	No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	3047	0.8897597327508673	RNA PCR Primer, Index 27 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1880	0.5489820471190123	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1755	0.5124805812201417	No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG	1366	0.39888801934285684	No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA	1161	0.3390256152687092	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1053	0.3074883487320851	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	981	0.28646350437433565	No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT	931	0.27186291801478746	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	815	0.23798955766063562	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	810	0.23652949902468082	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	778	0.22718512375456998	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	773	0.22572506511861515	No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT	754	0.22017684230198684	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	709	0.20703631457839347	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	691	0.20178010348895614	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	679	0.19827596276266451	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	665	0.19418779858199106	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	615	0.17958721222244284	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	509	0.1486339691402007	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	500	0.146005863595482	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	486	0.1419176994148085	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	471	0.13753752350694404	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	439	0.1281931482368332	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	436	0.1273171130552603	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	421	0.12293693714739584	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	398	0.11622066742200367	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	397	0.1159286556948127	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	395	0.11534463224043078	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	377	0.11008842115099342	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	374	0.10921238596942054	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	369	0.10775232733346571	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	366	0.10687629215189283	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	364	0.10629226869751089	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	345	0.10074404588088258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.92011727190964E-4	0.0
2	0.0	0.0	0.0	2.92011727190964E-4	0.0
3	0.0	0.0	0.0	2.92011727190964E-4	0.0
4	0.0	0.0	0.0	2.92011727190964E-4	0.0
5	0.0	0.0	0.0	2.92011727190964E-4	0.0
6	0.0	0.0	0.0	2.92011727190964E-4	0.0
7	0.0	0.0	0.0	5.84023454381928E-4	0.0
8	0.0	0.0	0.0	5.84023454381928E-4	0.0
9	0.0	0.0	0.0	5.84023454381928E-4	0.0
10	0.0	0.0	0.0	5.84023454381928E-4	0.0
11	0.0	0.0	0.0	5.84023454381928E-4	0.0
12	0.0	0.0	0.0	5.84023454381928E-4	0.0
13	0.0	0.0	0.0	5.84023454381928E-4	0.0
14	0.0	0.0	0.0	5.84023454381928E-4	0.0
15	0.0	0.0	0.0	5.84023454381928E-4	0.0
16	0.0	0.0	0.0	5.84023454381928E-4	0.0
17	0.0	0.0	0.0	8.76035181572892E-4	0.0
18	0.0	0.0	0.0	8.76035181572892E-4	0.0
19	0.0	0.0	0.0	8.76035181572892E-4	0.0
20	0.0	0.0	0.0	0.001168046908763856	0.0
21	0.0	0.0	0.0	0.001168046908763856	0.0
22	0.0	0.0	0.0	0.002044082090336748	0.0
23	0.0	0.0	0.0	0.002336093817527712	0.0
24	0.0	0.0	0.0	0.003504140726291568	0.0
25	0.0	0.0	0.0	0.003796152453482532	0.0
26	0.0	0.0	0.0	0.004672187635055424	0.0
27	0.0	0.0	0.0	0.007008281452583136	0.0
28	0.0	0.0	0.0	0.021316856084940373	0.0
29	0.0	0.0	0.0	0.0543141812575193	0.0
30	0.0	0.0	0.0	0.09782392860897293	0.0
31	0.0	0.0	0.0	0.16002242650064827	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATAGT	20	0.0018401322	37.0	4
GCGTCAC	20	0.0018401322	37.0	26
GATTACG	60	3.45608E-11	33.916668	1
TTATACA	1830	0.0	33.25956	2
TATACAC	1875	0.0	32.65867	3
CTTATAC	1870	0.0	32.64706	1
CGTCTTC	540	0.0	31.518518	37
ATTACGG	65	8.731149E-11	31.307692	2
AGGCGTC	25	0.005491024	29.599998	24
GACTTGC	25	0.005491024	29.599998	15
CTTAGGA	60	4.3042746E-8	27.750002	2
GACCGTT	55	6.2401887E-7	26.90909	7
CTCTTAC	70	6.557457E-9	26.42857	31
AGCTAGT	35	8.856633E-4	26.42857	6
TAGCTAG	35	8.856633E-4	26.42857	5
CTGTGCA	50	9.066778E-6	25.899998	9
TAGGACC	80	9.731593E-10	25.4375	4
CTTACTT	80	9.731593E-10	25.4375	33
CCGTCTT	1645	0.0	25.19149	37
GTACATG	60	1.3328299E-6	24.666668	1
>>END_MODULE