FastQCFastQC Report
Fri 10 Feb 2017
ERR1630914.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630914.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400516
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT196664.910165886007051No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG167754.188347032328296No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT138163.4495500803962886Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC89172.226377972415584No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC57501.4356480140618602Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC42161.0526420917017048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32700.8164467836490927No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31040.7750002496779155No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG20530.5125887604989564No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16200.40447822309221104No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA15800.39449110647265023No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT13160.32857613678354924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11900.29711671943193285No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10970.273896673291454No Hit
ACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT10550.2634102008409152No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9490.2369443417990792No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9180.22920432641891958No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8350.20848105943333098No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7830.195497807827902No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7110.17752099791269263No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6770.16903194878606598No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6660.16628549171568677No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6660.16628549171568677No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4730.11809765402630606No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4700.11734862027983901No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4680.11684926444886097No Hit
ATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT4590.11460216320945979Illumina PCR Primer Index 10 (95% over 21bp)
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4570.11410280737848176No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4360.10885957115321235No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4180.10436536867441No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4160.10386601284343197No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4160.10386601284343197No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4140.10336665701245393No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4100.10236794535049785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG251.2321897E-437.02
GCAACGC251.2321897E-437.03
TAGGTCG251.2321897E-437.021
CATTGCG251.2321897E-437.029
TGTGCGC251.2321897E-437.010
GCTCTAG200.001840504837.01
CAGCTCG251.2321897E-437.037
GTATACA251.2321897E-437.02
ACGAGCC200.001840504837.04
CTGTGCG251.2321897E-437.09
CTTATAC22750.035.129671
TTATACA23750.033.4168432
GATTACG401.5966234E-632.3751
CGTCTTC6450.032.1240337
CGTTATT352.3844521E-531.71428710
TCAGAGT352.3844521E-531.7142873
TGTTACG352.3844521E-531.71428716
TTGTTAC352.3844521E-531.71428715
GTAAGGC352.3844521E-531.71428716
TATACAC25600.031.4355473
Produced by FastQC (version 0.11.5)