FastQCFastQC Report
Fri 10 Feb 2017
ERR1630913.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630913.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208540
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT175298.405581662990313No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG136756.557494964994726No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT104074.990409513762348RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC72973.4990889038074227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38241.8337009686391101No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC37061.7771170998369619RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC35931.7229308525942264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33821.6217512227869952No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17950.8607461398292894No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG16270.7801860554330105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13740.6588664045267095No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA13570.650714491224705No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT11380.545698666922413No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10420.49966433298168217No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8210.3936894600556248No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT8100.38841469262491607RNA PCR Primer, Index 26 (95% over 21bp)
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7290.34957322336242447No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7280.3490936990505419No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6800.32607653208017645No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6760.324158434832646No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6720.32224033758511555No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6650.3188836674019373No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6400.30689555960487197No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5890.28243981969885873No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4920.23592596144624534No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4640.2224992807135322No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4530.21722451328282344No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4360.209072599980819No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4270.20475688117387553No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4260.2042773568619929No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.200441162366932No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3890.18653495732233624No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3820.18317828713915796No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3780.1812601898916275No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3780.1812601898916275No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3680.17646494677280136No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3660.17550589814903617No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3370.1615996931044404No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT3220.1544068284262012RNA PCR Primer, Index 26 (95% over 23bp)
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3140.1505706339311403No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3140.1505706339311403No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3070.14721396374796203No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3030.14529586650043158No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2940.14098014769348804No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2760.13234871007960103No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2750.13186918576771842No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2710.12995108852018797No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2690.12899203989642274No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2550.12227869953006618No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.12084012659441834No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA2520.12084012659441834No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2510.12036060228253573No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2450.11748345641124006No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2450.11748345641124006No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2430.11652440778747483No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2390.11460631053994438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2370.11364726191617915No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC2330.1117291646686487No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2260.10837249448547041No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2210.10597487292605735No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2190.10501582430229213No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2110.10117962980723122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCAA200.00183848337.02
ATTACGG650.037.02
ACTCAGA403.8076905E-837.01
GATCAAC200.00183848337.03
GATTACG650.037.01
GTTGTAT200.00183848337.01
CTATACA251.2301626E-436.9999961
TTATCAA251.2301626E-436.9999962
TGTATCC251.2301626E-436.9999963
CTTATAC18750.035.9146651
CGTCTTC4050.034.7160537
TTACGGG700.034.3571433
TTATACA19700.034.1827432
TCAGAGT451.0727672E-732.888893
CAGACAC451.0727672E-732.8888918
GATACAC401.5917649E-632.3753
TGATACA401.5917649E-632.3752
TATACAC21000.032.330953
TTGTATC303.589468E-430.8333342
TGTGAAA250.005486157729.59999816
Produced by FastQC (version 0.11.5)