FastQCFastQC Report
Fri 10 Feb 2017
ERR1630912.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630912.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199590
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG109685.495265293852397No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT92954.657046946239792TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC59332.9725938173255173No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT56102.8107620622275666No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC36861.8467859111177913RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC29951.5005761811714013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.9684853950598726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16810.8422265644571372No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG13500.676386592514655No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA8790.4404028257928754No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT8630.43238639210381286No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT7650.3832857357583045No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7030.352222055213187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6760.33869432336289396No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4800.24049301067187737No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4180.20942933012675988No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4080.20441905907109575No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3250.16283380930908362No Hit
ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT3160.15832456535898592TruSeq Adapter, Index 12 (95% over 21bp)
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3070.15381532140888823No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2860.14329375219199358No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2770.13878450824189587No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2730.13678039981963025No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.13177012876396613No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2560.12826293902500127No Hit
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT2500.1252567763916028TruSeq Adapter, Index 12 (95% over 24bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2380.11924445112480586No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA2250.11273109875244251No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2120.10621774638007916No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2110.10571671927451275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC7400.033.251
GGTATCA2200.031.9545461
TTATACA7700.031.7142892
TGATACA352.3785013E-531.7142872
CGTCTTC4850.031.2783537
ATCTATA250.005485598529.61
CTATACA250.005485598529.61
CCGTCTG250.005485598529.69
CTGCGAG250.005485598529.61
TAGGAGC250.005485598529.66
CAGACAC250.005485598529.618
TATACAC8900.027.8539333
GATACAC405.915936E-527.753
GATCAAC358.8436255E-426.4285723
CGGATCG358.8436255E-426.42857237
ATAAAGA358.8436255E-426.42857235
CCGTCTT14750.024.70847537
GCCGTCT15050.024.21594836
GATAAAG400.0019256323.12534
CATGCTA400.0019256323.1254
Produced by FastQC (version 0.11.5)