##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630912.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	199590
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.462463049250964	31.0	30.0	33.0	27.0	34.0
2	30.456490806152612	31.0	30.0	34.0	27.0	34.0
3	29.890736008818077	31.0	30.0	34.0	25.0	34.0
4	34.116183175509796	35.0	33.0	37.0	28.0	37.0
5	34.02977604088381	35.0	33.0	37.0	30.0	37.0
6	34.302540207425224	35.0	35.0	37.0	30.0	37.0
7	34.29628738914775	35.0	35.0	37.0	30.0	37.0
8	34.39467408186783	35.0	35.0	37.0	30.0	37.0
9	36.04848439300566	37.0	35.0	39.0	32.0	39.0
10	35.77706297910717	37.0	35.0	39.0	30.0	39.0
11	35.84475675134025	37.0	35.0	39.0	30.0	39.0
12	35.70612756150108	37.0	35.0	39.0	30.0	39.0
13	35.90075154065835	37.0	35.0	39.0	31.0	39.0
14	36.53309785059372	38.0	36.0	40.0	31.0	41.0
15	36.570228969387244	38.0	36.0	40.0	31.0	41.0
16	36.5931860313643	38.0	36.0	40.0	31.0	41.0
17	36.52508642717571	38.0	36.0	40.0	31.0	41.0
18	36.69161280625282	38.0	36.0	40.0	31.0	41.0
19	36.63183526228769	38.0	36.0	40.0	31.0	41.0
20	36.60414850443409	38.0	35.0	40.0	31.0	41.0
21	36.51905406082469	38.0	35.0	40.0	30.0	41.0
22	36.12869382233579	38.0	35.0	40.0	30.0	41.0
23	36.23820331679944	38.0	35.0	40.0	30.0	41.0
24	36.02131870334185	38.0	34.0	40.0	30.0	41.0
25	35.89875244250714	38.0	34.0	40.0	29.0	41.0
26	35.75878551029611	38.0	34.0	40.0	29.0	41.0
27	35.527736860564154	38.0	34.0	40.0	27.0	41.0
28	35.53412996643118	38.0	34.0	40.0	28.0	41.0
29	35.24712159927852	38.0	34.0	40.0	27.0	41.0
30	35.02952552733103	37.0	33.0	40.0	27.0	40.0
31	34.99702890926399	37.0	33.0	40.0	27.0	40.0
32	34.51460995039832	37.0	33.0	39.0	25.0	40.0
33	34.517430733002655	37.0	33.0	39.0	25.0	40.0
34	34.54708151711007	37.0	33.0	39.0	26.0	40.0
35	34.358940828698834	37.0	33.0	39.0	25.0	40.0
36	34.482594318352625	37.0	33.0	39.0	25.0	40.0
37	34.54147502379879	37.0	33.0	40.0	25.0	40.0
38	34.40302620371762	37.0	33.0	39.0	25.0	40.0
39	34.11922441004058	37.0	32.0	39.0	24.0	40.0
40	33.75156570970489	36.0	32.0	39.0	23.0	40.0
41	33.75224710656847	36.0	32.0	39.0	23.0	40.0
42	33.5994238188286	36.0	31.0	39.0	22.0	40.0
43	32.57436745327922	35.0	30.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	11.0
10	16.0
11	14.0
12	14.0
13	11.0
14	11.0
15	12.0
16	9.0
17	12.0
18	38.0
19	69.0
20	103.0
21	204.0
22	363.0
23	676.0
24	1063.0
25	1615.0
26	2418.0
27	3312.0
28	4518.0
29	5843.0
30	7553.0
31	9557.0
32	11841.0
33	14851.0
34	18658.0
35	23130.0
36	28472.0
37	31198.0
38	25940.0
39	8055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.626835011774133	26.02535197154166	18.342101307680743	24.005711709003457
2	13.577333533744177	21.571722030161833	40.4013227115587	24.449621724535298
3	14.457137131118794	32.120346710757055	34.088381181421916	19.334134976702238
4	10.054110927401172	19.29204869983466	30.75404579387745	39.89979457888671
5	12.16694223157473	39.52252116839521	31.34124956160128	16.969287038428778
6	25.876546921188435	37.307981361791676	16.392103812816273	20.42336790420362
7	21.726038378676286	35.54536800440904	18.538002905957214	24.190590710957462
8	26.80144295806403	29.0275063880956	25.01878851645874	19.15226213738163
9	20.56565960218448	12.677488852146901	15.87754897539957	50.87930257026905
10	15.453680044090385	24.945638559046042	30.782604338894735	28.81807705796884
11	29.764016233278216	20.99554085876046	21.688461345758807	27.551981562202517
12	18.290495515807407	29.51600781602285	22.20602234580891	29.98747432236084
13	34.53579838669272	16.751841274612957	22.67047447266897	26.04188586602535
14	23.114885515306376	25.519314594919585	22.321258580089182	29.044541309684853
15	28.140688411243048	27.58905756801443	18.73240142291698	25.537852597825545
16	27.301969036524877	23.252166942231575	19.436344506237788	30.00951951500576
17	18.98191292148905	33.272709053559794	19.838669272007618	27.906708752943533
18	25.717220301618315	19.36369557593066	21.78566060423869	33.13342351821233
19	28.917781451976555	26.427175710205926	22.21253569818127	22.442507139636252
20	22.14690114735207	21.73555789368205	22.567763916027857	33.549777042938025
21	34.4345909113683	20.687910215942683	23.921539155268302	20.955959717420715
22	18.55654090886317	33.910516558945844	27.373615912620874	20.15932661957012
23	28.64622476075956	24.689613708101607	23.31279122200511	23.351370309133724
24	29.380730497519913	26.906157623127413	25.268300015030814	18.44481186432186
25	19.207876146099505	27.64116438699334	28.04699634250213	25.10396312440503
26	31.925948193797282	27.98436795430633	23.184027255874543	16.905656596021846
27	18.415251265093442	28.5575429630743	29.182824790821183	23.844380981011074
28	24.167042436995843	24.5167593566812	28.649230923392956	22.666967282930006
29	25.4952652938524	21.4364447116589	30.465955208176766	22.60233478631194
30	18.281477027907208	26.420662357833557	32.90495515807405	22.39290545618518
31	30.830201913923545	22.647928252918483	23.23362893932562	23.288240893832356
32	21.72704043288742	23.409990480484993	36.374567864121445	18.488401222506138
33	17.769928353123905	31.07470314143995	31.92745127511398	19.227917230322163
34	22.268149706899145	30.07264893030713	26.40913873440553	21.250062628388196
35	19.423317801493063	21.281126308933313	41.429931359286535	17.86562453028709
36	23.75670123753695	25.449170800140287	22.487098552031664	28.307029410291097
37	21.14885515306378	23.183526228768976	30.753043739666314	24.91457487850093
38	21.377323513202064	20.038579087128614	28.95986772884413	29.62422967082519
39	28.869682849842178	21.878851645874043	26.147602585299868	23.103862918983918
40	18.537501878851646	20.141289643769728	39.465905105466206	21.85530337191242
41	23.76672177964828	24.107921238539003	24.373966631594772	27.751390350217946
42	20.721980059121197	20.586702740618268	36.49882258630192	22.192494613958615
43	24.858459842677487	19.443358885715718	31.07821033117892	24.619970940427876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	10.5
2	10.0
3	33.0
4	56.0
5	56.0
6	81.5
7	107.0
8	121.5
9	136.0
10	208.0
11	280.0
12	280.0
13	478.5
14	677.0
15	1756.5
16	2836.0
17	2776.5
18	2717.0
19	2717.0
20	2213.0
21	1709.0
22	1335.0
23	961.0
24	992.0
25	1023.0
26	1023.0
27	1242.0
28	1461.0
29	1897.0
30	2333.0
31	2917.0
32	3501.0
33	3501.0
34	4366.5
35	5232.0
36	5832.0
37	6432.0
38	7682.5
39	8933.0
40	8933.0
41	9651.0
42	10369.0
43	11416.5
44	12464.0
45	13525.5
46	14587.0
47	14587.0
48	16394.0
49	18201.0
50	25309.5
51	32418.0
52	23622.5
53	14827.0
54	14827.0
55	21819.5
56	28812.0
57	23019.5
58	17227.0
59	11216.5
60	5206.0
61	5206.0
62	4338.0
63	3470.0
64	2579.0
65	1688.0
66	1315.0
67	942.0
68	942.0
69	745.5
70	549.0
71	402.0
72	255.0
73	157.5
74	60.0
75	60.0
76	48.5
77	37.0
78	32.5
79	28.0
80	17.5
81	7.0
82	7.0
83	6.0
84	5.0
85	10.0
86	15.0
87	10.0
88	5.0
89	5.0
90	4.0
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	199590.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.681146350017535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.42048890883197	18.34160028057518
2	10.466863062606082	5.376020842727591
3	4.543769631464971	3.500676386592515
4	2.6962171020543537	2.769677839571121
5	1.8358468111672552	2.3573325316899645
6	1.2993347250131688	2.002104313843379
7	0.9813293794018377	1.7641164386993338
8	0.8408607604814952	1.7275414599929855
9	0.659422127709386	1.5241244551330226
>10	4.668630626060831	23.225111478530987
>50	0.40189632635542466	6.994839420812666
>100	0.1580271962853854	6.69973445563405
>500	0.009754765202801568	1.9469913322310735
>1k	0.009754765202801568	5.834460644320857
>5k	0.005852859121680941	10.440402825792876
>10k+	0.0019509530405603139	5.495265293852397
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	10968	5.495265293852397	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	9295	4.657046946239792	TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	5933	2.9725938173255173	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	5610	2.8107620622275666	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	3686	1.8467859111177913	RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	2995	1.5005761811714013	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1933	0.9684853950598726	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1681	0.8422265644571372	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	1350	0.676386592514655	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	879	0.4404028257928754	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	863	0.43238639210381286	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	765	0.3832857357583045	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	703	0.352222055213187	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	676	0.33869432336289396	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	480	0.24049301067187737	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	418	0.20942933012675988	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	408	0.20441905907109575	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	325	0.16283380930908362	No Hit
ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT	316	0.15832456535898592	TruSeq Adapter, Index 12 (95% over 21bp)
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	307	0.15381532140888823	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	286	0.14329375219199358	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	277	0.13878450824189587	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	273	0.13678039981963025	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	263	0.13177012876396613	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	256	0.12826293902500127	No Hit
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT	250	0.1252567763916028	TruSeq Adapter, Index 12 (95% over 24bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	238	0.11924445112480586	No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	225	0.11273109875244251	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	212	0.10621774638007916	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	211	0.10571671927451275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0015030813166992333	0.0
22	0.0	0.0	0.0	0.0015030813166992333	0.0
23	0.0	0.0	0.0	0.0015030813166992333	0.0
24	0.0	0.0	0.0	0.0015030813166992333	0.0
25	0.0	0.0	0.0	0.0020041084222656444	0.0
26	0.0	0.0	0.0	0.0025051355278320558	0.0
27	0.0	0.0	0.0	0.0025051355278320558	0.0
28	0.0	0.0	0.0	0.007515406583496167	0.0
29	0.0	0.0	0.0	0.015531840272558746	0.0
30	0.0	0.0	0.0	0.033568816072949544	0.0
31	0.0	0.0	0.0	0.06563455082919986	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	740	0.0	33.25	1
GGTATCA	220	0.0	31.954546	1
TTATACA	770	0.0	31.714289	2
TGATACA	35	2.3785013E-5	31.714287	2
CGTCTTC	485	0.0	31.27835	37
ATCTATA	25	0.0054855985	29.6	1
CTATACA	25	0.0054855985	29.6	1
CCGTCTG	25	0.0054855985	29.6	9
CTGCGAG	25	0.0054855985	29.6	1
TAGGAGC	25	0.0054855985	29.6	6
CAGACAC	25	0.0054855985	29.6	18
TATACAC	890	0.0	27.853933	3
GATACAC	40	5.915936E-5	27.75	3
GATCAAC	35	8.8436255E-4	26.428572	3
CGGATCG	35	8.8436255E-4	26.428572	37
ATAAAGA	35	8.8436255E-4	26.428572	35
CCGTCTT	1475	0.0	24.708475	37
GCCGTCT	1505	0.0	24.215948	36
GATAAAG	40	0.00192563	23.125	34
CATGCTA	40	0.00192563	23.125	4
>>END_MODULE