FastQCFastQC Report
Fri 10 Feb 2017
ERR1630911.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630911.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530882
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT179383.378905293455043No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG133622.516943501569087No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT121932.2967439091926263RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC70581.3294856484115114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61351.155624036979969No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59201.1151253950972155No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC50010.9420172467704688RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC37070.6982719323691517No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23400.44077591630531837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22730.4281554092999951No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG16290.30684784942793314No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14500.2731303754883383No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA13980.26333535512599787No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11770.22170651858605112No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT11200.21096966934271646No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10580.1992909912183875No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10380.19552367569441043No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10100.1902494339608425No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9990.18817741042265512No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9600.18083114515089982No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT8810.16595024883119036No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8630.16255966485961099No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8330.15690869157364537No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8110.15276464449727056No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7090.13355133532498747No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6890.1297840198010104No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6370.11998899943866999No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6030.11358456304790894No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5960.11226600261451697No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5830.10981724752393188No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5740.10812195553814219No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5610.10567320044755708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG200.001841044337.09
CGCCTAT251.2327309E-437.034
CAAATAC200.001841044337.03
TCTATCG200.001841044337.029
CTTATAC21850.034.0366131
TTATACA22250.033.0921362
TATACAC24050.030.8461533
CGTCTTC6150.030.68292837
GCAAATA250.005493712629.62
GCGCCAG250.005493712629.613
TTAAGCC250.005493712629.631
CCGAGCT250.005493712629.623
GGACAGC250.005493712629.66
TATCGCC250.005493712629.631
GATTACG1200.029.2916681
TTACGGG1150.028.9565223
TGTACTA405.9342023E-527.755
GGTATCA9150.027.0928961
GGCCAGC358.863084E-426.4285731
CCGTCTT19000.025.02368437
Produced by FastQC (version 0.11.5)