FastQCFastQC Report
Fri 10 Feb 2017
ERR1630910.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630910.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313164
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG155384.961617555019095No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT129304.128827068245392TruSeq Adapter, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT105353.3640520621782835No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC82632.638553601307941No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC56471.8032085424889197RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC41021.30985681623686TruSeq Adapter, Index 11 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG19300.6162905059329936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18730.598089180110102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17870.5706275306229325No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA14500.4630161832139071No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT12400.39595866702430677No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT9450.30175882285320155No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9320.29760764327955963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7650.24428095183354406No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6610.21107151524440868No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5840.1864837593082219No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5350.17083700553064848No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4960.1583834668097227No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4670.14912314314544456No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4360.1392241764698369No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4230.13507299689619498No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT4120.13156046033388258TruSeq Adapter, Index 11 (95% over 22bp)
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4070.12996385280555875No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3930.12549335172625206No Hit
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT3440.10984659794867864TruSeq Adapter, Index 11 (96% over 25bp)
CTTTACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA3420.10920795493734912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACA501.7826096E-1037.02
GCTCTAG251.2315753E-437.01
CTTATAA251.2315753E-437.01
ATGAGAG251.2315753E-437.029
ATAACAC251.2315753E-437.04
CTTATAC13150.034.749051
GATACAC554.965841E-1033.6363643
CGTCTTC6400.032.95312537
CGCCGAG352.3828026E-531.7142872
TTATACA14350.031.5853652
AGAGTAG303.5935524E-430.83333432
TTGTATT250.005490313329.69
ATATTCC250.005490313329.66
ATTTCGG250.005490313329.629
GGTATCA2500.029.5999981
TATACAC16200.028.4351843
AGTAGCT358.854932E-426.42857234
TAGGACC358.854932E-426.4285724
CCATTTC358.854932E-426.42857226
CGGATCG659.346331E-825.61538537
Produced by FastQC (version 0.11.5)