##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630910.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	313164
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.504674866842933	31.0	30.0	33.0	27.0	34.0
2	30.510607860418183	31.0	30.0	34.0	27.0	34.0
3	29.917027499968068	31.0	30.0	34.0	25.0	34.0
4	34.15361280351509	35.0	35.0	37.0	30.0	37.0
5	34.095145035827876	35.0	33.0	37.0	30.0	37.0
6	34.38236515052816	35.0	35.0	37.0	30.0	37.0
7	34.412617669974836	35.0	35.0	37.0	30.0	37.0
8	34.46672350589468	35.0	35.0	37.0	30.0	37.0
9	36.14738603415463	37.0	35.0	39.0	32.0	39.0
10	35.860925904637824	37.0	35.0	39.0	30.0	39.0
11	35.94625180416651	37.0	35.0	39.0	31.0	39.0
12	35.817817501373085	37.0	35.0	39.0	30.0	39.0
13	36.00863764672823	37.0	35.0	39.0	31.0	39.0
14	36.728541594819326	38.0	36.0	40.0	31.0	41.0
15	36.737667803451224	38.0	36.0	40.0	31.0	41.0
16	36.73513877712636	38.0	36.0	40.0	31.0	41.0
17	36.605609201568505	38.0	36.0	40.0	31.0	41.0
18	36.785055114891875	38.0	36.0	40.0	31.0	41.0
19	36.730786425004155	38.0	36.0	40.0	31.0	41.0
20	36.690082512677066	38.0	36.0	40.0	31.0	41.0
21	36.54236438415654	38.0	35.0	40.0	30.0	41.0
22	36.2857161104086	38.0	35.0	40.0	30.0	41.0
23	36.415858783257335	38.0	35.0	40.0	30.0	41.0
24	36.28275280683604	38.0	35.0	40.0	30.0	41.0
25	36.237214366913186	38.0	35.0	40.0	30.0	41.0
26	36.05975463335505	38.0	35.0	40.0	30.0	41.0
27	35.81983561328888	38.0	34.0	40.0	29.0	41.0
28	35.83123219782606	38.0	34.0	40.0	29.0	41.0
29	35.68189510927182	38.0	34.0	40.0	29.0	41.0
30	35.582477551698155	38.0	34.0	40.0	28.0	41.0
31	35.577473783704384	38.0	34.0	40.0	28.0	41.0
32	35.31523738360731	38.0	34.0	40.0	27.0	41.0
33	35.31571955908087	38.0	34.0	40.0	27.0	41.0
34	35.317993128201195	38.0	34.0	40.0	27.0	41.0
35	35.33094480847096	38.0	34.0	40.0	27.0	41.0
36	35.35993281475521	38.0	34.0	40.0	27.0	41.0
37	35.292447407748014	38.0	34.0	40.0	27.0	41.0
38	35.17078272087468	38.0	33.0	40.0	27.0	41.0
39	35.010809032966755	38.0	33.0	40.0	26.0	41.0
40	34.70685966458469	37.0	33.0	40.0	26.0	40.0
41	34.701661110472465	37.0	33.0	40.0	26.0	40.0
42	34.585479173851404	37.0	33.0	40.0	25.0	41.0
43	33.613716774597336	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	13.0
11	26.0
12	20.0
13	6.0
14	21.0
15	13.0
16	10.0
17	26.0
18	26.0
19	60.0
20	119.0
21	220.0
22	411.0
23	758.0
24	1216.0
25	1922.0
26	2948.0
27	4361.0
28	6038.0
29	8195.0
30	10598.0
31	13472.0
32	17063.0
33	21762.0
34	27232.0
35	35254.0
36	44568.0
37	51477.0
38	48007.0
39	17311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.5561047885453	25.416714564892516	17.39344241355967	24.633738233002518
2	14.070263504106476	21.050631617938205	40.84249786054591	24.03660701740941
3	14.378089435567306	31.90117638042687	33.76920718856574	19.951526995440087
4	10.469913527736265	19.920552809390607	32.26903475495268	37.34049890792045
5	12.346246695022417	38.929762041613984	32.92013130500313	15.803859958360475
6	26.040349465455797	38.349554865821105	16.53287095579313	19.077224712929965
7	22.698330587168382	34.392522767623355	19.773664916784817	23.135481728423446
8	26.014803745002617	30.575672810412435	24.472800194147474	18.93672325043747
9	21.020615396405717	13.034065218224317	16.525207239657178	49.42011214571279
10	15.211837886858003	25.62491218658594	31.619215490924883	27.544034435631172
11	29.4794420814653	21.78283583042751	22.753892529154054	25.983829558953136
12	18.346936429474653	28.58693847313229	23.12909529831015	29.93702979908291
13	33.620403366925956	16.968425489519866	23.785620313956905	25.62555082959727
14	23.12302818970252	24.761786156774086	22.246171335147082	29.869014318376312
15	27.6733596454254	27.353399496749308	18.92395039021088	26.049290467614412
16	27.509867034525044	23.723991263363605	20.058499699837785	28.70764200227357
17	19.26051525718154	33.11108556539066	20.32704908610185	27.30135009132595
18	26.085373797754528	20.412946571125673	21.90769053914243	31.593989091977363
19	29.654110945063927	26.550944553013757	22.07182179305412	21.723122708868196
20	24.810642347140796	24.4019108198899	22.336219999744543	28.451226833224762
21	29.88466107215389	25.884520570691393	22.11333358879054	22.11748476836418
22	24.540496353348406	32.27127000549233	22.22477679426754	20.963456846891724
23	25.326027257283723	22.123232555466146	24.608192512549333	27.942547674700798
24	26.27249620007408	29.18375036721973	25.772438722203063	18.771314710503123
25	22.439680167579926	29.983970060415626	22.599660241917974	24.97668953008647
26	27.92881684995721	26.27377348609674	23.453845269571218	22.34356439437483
27	26.391922443192705	29.873804140961287	24.309307583247115	19.424965832598893
28	18.660829469543113	26.26834502050044	28.0476044500645	27.023221059891945
29	29.103600669297876	22.642768645182716	26.228429832292342	22.025200853227066
30	22.494603466554267	24.1327227906145	27.519446679694987	25.85322706313625
31	22.430100522409983	23.75975527199806	27.394911292485723	26.41523291310623
32	25.204365763625447	28.539678890293903	26.577767559489594	19.67818778659105
33	17.933415079638785	22.584332809646064	30.52234611896642	28.95990599174873
34	19.343219527148715	29.805469338749024	28.999501858451165	21.851809275651096
35	19.972602214813964	25.12357742269226	36.157732050938165	18.746088311555607
36	23.98040643241241	22.632550357001442	21.849893346617108	31.537149863969038
37	21.558033490439517	24.028304658262126	29.531172165382998	24.882489685915367
38	21.660535693757904	20.939507733966867	28.654315310827556	28.745641261447673
39	27.866868477858247	22.553677945102248	25.71176763612676	23.867685940912747
40	19.204633993690205	20.740251114432056	38.39649512715383	21.658619764723916
41	23.591792160018393	24.28216525526561	24.247039889642487	27.879002695073506
42	21.564739242058476	21.430624209679277	34.824245443282116	22.18039110498014
43	25.039276545196763	20.196765911790628	30.87264181068067	23.89131573233194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	3.5
2	7.0
3	28.0
4	49.0
5	49.0
6	73.0
7	97.0
8	121.0
9	145.0
10	221.5
11	298.0
12	298.0
13	492.0
14	686.0
15	1736.5
16	2787.0
17	2848.5
18	2910.0
19	2910.0
20	2426.0
21	1942.0
22	1569.5
23	1197.0
24	1324.0
25	1451.0
26	1451.0
27	1778.0
28	2105.0
29	2957.0
30	3809.0
31	4818.0
32	5827.0
33	5827.0
34	7726.5
35	9626.0
36	10831.5
37	12037.0
38	13855.5
39	15674.0
40	15674.0
41	17117.0
42	18560.0
43	20109.5
44	21659.0
45	23181.0
46	24703.0
47	24703.0
48	25815.5
49	26928.0
50	27157.0
51	27386.0
52	38006.5
53	48627.0
54	48627.0
55	34367.0
56	20107.0
57	27540.0
58	34973.0
59	27342.5
60	19712.0
61	19712.0
62	12412.5
63	5113.0
64	3732.0
65	2351.0
66	1853.5
67	1356.0
68	1356.0
69	986.5
70	617.0
71	458.5
72	300.0
73	186.5
74	73.0
75	73.0
76	52.0
77	31.0
78	20.0
79	9.0
80	7.5
81	6.0
82	6.0
83	5.0
84	4.0
85	2.5
86	1.0
87	1.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	313164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.659897050746572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.99307101472778	15.160427124445977
2	10.32713655997995	4.473694294363336
3	4.5672332709233245	2.9677740736483122
4	2.8069761613421593	2.431952587142839
5	1.8221757013754774	1.9734069050082386
6	1.3297754713921364	1.7281679886577002
7	1.1012663826274123	1.6697321531210483
8	0.8078901976972181	1.3999054808343232
9	0.6471967094691218	1.2616392688814806
>10	5.649334375138211	24.83044028049201
>50	0.6442482050979641	9.621156965679324
>100	0.2786336630744055	10.21956546729509
>500	0.00884551311347319	1.4120396980495842
>1k	0.00884551311347319	3.9538388831411018
>5k	0.00294850437115773	4.44176214379686
>10k+	0.004422756556736595	12.454496685442772
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	15538	4.961617555019095	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	12930	4.128827068245392	TruSeq Adapter, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	10535	3.3640520621782835	No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	8263	2.638553601307941	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	5647	1.8032085424889197	RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	4102	1.30985681623686	TruSeq Adapter, Index 11 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG	1930	0.6162905059329936	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1873	0.598089180110102	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1787	0.5706275306229325	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA	1450	0.4630161832139071	No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT	1240	0.39595866702430677	No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT	945	0.30175882285320155	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	932	0.29760764327955963	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	765	0.24428095183354406	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	661	0.21107151524440868	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	584	0.1864837593082219	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	535	0.17083700553064848	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	496	0.1583834668097227	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	467	0.14912314314544456	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	436	0.1392241764698369	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	423	0.13507299689619498	No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT	412	0.13156046033388258	TruSeq Adapter, Index 11 (95% over 22bp)
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	407	0.12996385280555875	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	393	0.12549335172625206	No Hit
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT	344	0.10984659794867864	TruSeq Adapter, Index 11 (96% over 25bp)
CTTTACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA	342	0.10920795493734912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	9.579645169942905E-4	0.0
22	0.0	0.0	0.0	9.579645169942905E-4	0.0
23	0.0	0.0	0.0	0.001277286022659054	0.0
24	0.0	0.0	0.0	0.0022352505396533444	0.0
25	0.0	0.0	0.0	0.0022352505396533444	0.0
26	0.0	0.0	0.0	0.0022352505396533444	0.0
27	0.0	0.0	0.0	0.002554572045318108	0.0
28	0.0	0.0	0.0	0.007025073124624797	0.0
29	0.0	0.0	0.0	0.0166047182945677	0.0
30	0.0	0.0	0.0	0.0332094365891354	0.0
31	0.0	0.0	0.0	0.07248598178590132	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATACA	50	1.7826096E-10	37.0	2
GCTCTAG	25	1.2315753E-4	37.0	1
CTTATAA	25	1.2315753E-4	37.0	1
ATGAGAG	25	1.2315753E-4	37.0	29
ATAACAC	25	1.2315753E-4	37.0	4
CTTATAC	1315	0.0	34.74905	1
GATACAC	55	4.965841E-10	33.636364	3
CGTCTTC	640	0.0	32.953125	37
CGCCGAG	35	2.3828026E-5	31.714287	2
TTATACA	1435	0.0	31.585365	2
AGAGTAG	30	3.5935524E-4	30.833334	32
TTGTATT	25	0.0054903133	29.6	9
ATATTCC	25	0.0054903133	29.6	6
ATTTCGG	25	0.0054903133	29.6	29
GGTATCA	250	0.0	29.599998	1
TATACAC	1620	0.0	28.435184	3
AGTAGCT	35	8.854932E-4	26.428572	34
TAGGACC	35	8.854932E-4	26.428572	4
CCATTTC	35	8.854932E-4	26.428572	26
CGGATCG	65	9.346331E-8	25.615385	37
>>END_MODULE