FastQCFastQC Report
Fri 10 Feb 2017
ERR1630909.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630909.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234086
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG131505.617593534000324No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT120775.159214989362884TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT108754.645728492947036No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC72963.116803226164743No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC51342.19321104209564RNA PCR Primer, Index 11 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38191.6314516886956076No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC35621.521662978563434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31961.3653101851456302No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17620.7527148142135797No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG16250.6941893150380629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13990.5976436010696923No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12910.5515067112087011No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA11600.49554437258101725No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT11490.4908452449099904No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11320.4835829566911306No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9930.42420307066633633No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9080.3878916295720376No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT8280.353716155600933No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7800.3332108712182702No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7560.3229582290269388No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7250.30971523286313574No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7120.3041617183428313No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6710.28664678793264015No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6280.2682774706731714No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5510.23538357697598317No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4890.2088975846483771No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4800.2050528438266278No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4780.2041984569773502No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4770.2037712635527114No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4650.1986449424570457No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4490.19180984766282477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.19095546081354717No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4440.18967388053963075No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4400.1879651068410755No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4390.1875379134164367No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4240.18113001204685458No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4140.1768580778004665No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4130.17643088437582768No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3890.16617824218449628No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3850.16446946848594107No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT3830.16361508163666344No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3740.15977034081491417No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3690.15763437369172012No Hit
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT3630.15507121314388728TruSeq Adapter, Index 11 (95% over 23bp)
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3630.15507121314388728No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3570.15250805259605446No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3540.15122647232213804No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3500.14951769862358277No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3380.1443913775279171No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3380.1443913775279171No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3240.13841066958297377No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2980.12730364054236476No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2920.12474047999453193No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.12388609314525431No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2860.12217731944669907No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2850.12175012602206026No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA2820.12046854574814383No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2820.12046854574814383No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2730.11662380492639458No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2670.11406064437856171No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2580.11021590355681245No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2580.11021590355681245No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2540.10850712985825722No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2530.10807993643361842No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2520.10765274300897962No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2480.10594396931042438No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2450.10466238903650794No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2440.10423519561186913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2430.10380800218723032No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA2430.10380800218723032No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2420.10338080876259152No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2420.10338080876259152No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2420.10338080876259152No Hit
CTGATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT2380.1016720350640363No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2380.1016720350640363No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2350.10039045479011988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT308.283709E-637.010
TAGGACC355.605798E-737.04
GATACAC403.8109647E-837.03
CTTAGGA355.605798E-737.02
TGATACA403.8109647E-837.02
ATTACGG452.6011548E-937.02
ACTCAGA200.00183894337.01
TCAGAGT200.00183894337.03
TTAGGAC355.605798E-737.03
TTACGGG452.6011548E-937.03
TAGCAAC251.230624E-436.9999961
AGCAACG251.230624E-436.9999962
CTTATAC12100.036.235541
CGTCTTC6050.033.63636437
GATTACG507.2795956E-933.31
GACCGTT401.592869E-632.3757
TTATACA13500.032.340742
TTACTTT352.3802491E-531.7142853
ATTACTT352.3802491E-531.7142852
ACAGACA303.5908018E-430.83333417
Produced by FastQC (version 0.11.5)