##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630909.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 234086 Sequences flagged as poor quality 0 Sequence length 43 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.73323479405005 31.0 30.0 33.0 27.0 34.0 2 30.73206428406654 31.0 30.0 34.0 27.0 34.0 3 30.139593995369225 31.0 30.0 34.0 25.0 34.0 4 34.33885409635775 35.0 35.0 37.0 30.0 37.0 5 34.483587228625375 35.0 35.0 37.0 30.0 37.0 6 34.70560819527866 35.0 35.0 37.0 32.0 37.0 7 34.72138444845057 35.0 35.0 37.0 32.0 37.0 8 34.74679818528233 35.0 35.0 37.0 32.0 37.0 9 36.47860615329409 38.0 35.0 39.0 32.0 39.0 10 36.27076800833881 38.0 35.0 39.0 32.0 39.0 11 36.30942901326863 38.0 35.0 39.0 32.0 39.0 12 36.139470109276076 37.0 35.0 39.0 32.0 39.0 13 36.23198738925011 38.0 35.0 39.0 32.0 39.0 14 36.881116341857265 38.0 36.0 40.0 31.0 41.0 15 36.92614680074844 38.0 36.0 40.0 31.0 41.0 16 36.9552344010321 38.0 36.0 40.0 31.0 41.0 17 36.99736848850422 38.0 36.0 40.0 31.0 41.0 18 37.10350896678998 39.0 36.0 40.0 31.0 41.0 19 37.00337909998889 39.0 36.0 40.0 31.0 41.0 20 36.964739454730314 39.0 36.0 40.0 31.0 41.0 21 36.8784122074793 38.0 36.0 40.0 31.0 41.0 22 36.67253488034312 38.0 35.0 40.0 30.0 41.0 23 36.79851422126911 38.0 35.0 40.0 31.0 41.0 24 36.63891902975829 38.0 35.0 40.0 30.0 41.0 25 36.525076254026295 38.0 35.0 40.0 30.0 41.0 26 36.31963466418325 38.0 35.0 40.0 30.0 41.0 27 36.018194167955365 38.0 35.0 40.0 30.0 41.0 28 36.08167083892245 38.0 35.0 40.0 30.0 41.0 29 35.98597096793486 38.0 35.0 40.0 30.0 41.0 30 35.7334056714199 38.0 34.0 40.0 29.0 41.0 31 35.62947378313953 38.0 34.0 40.0 29.0 41.0 32 35.36535717642234 38.0 34.0 40.0 28.0 41.0 33 35.3599574515349 38.0 34.0 40.0 28.0 41.0 34 35.21794981331647 38.0 34.0 40.0 27.0 41.0 35 35.18958416992046 38.0 34.0 40.0 27.0 41.0 36 35.07820202831438 38.0 33.0 40.0 26.0 41.0 37 34.929406286578434 37.0 33.0 40.0 26.0 41.0 38 34.75178353254787 37.0 33.0 40.0 25.0 41.0 39 34.513443777073384 37.0 33.0 40.0 24.0 41.0 40 34.12631682373145 37.0 33.0 40.0 23.0 41.0 41 34.0753953675145 37.0 33.0 40.0 23.0 41.0 42 33.85774458959528 37.0 32.0 40.0 22.0 41.0 43 32.75059166289312 36.0 31.0 39.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 12.0 10 16.0 11 15.0 12 12.0 13 19.0 14 9.0 15 13.0 16 14.0 17 7.0 18 27.0 19 72.0 20 93.0 21 184.0 22 359.0 23 613.0 24 1045.0 25 1619.0 26 2378.0 27 3407.0 28 4718.0 29 6123.0 30 7661.0 31 9681.0 32 11940.0 33 14810.0 34 18761.0 35 23822.0 36 31477.0 37 39774.0 38 40375.0 39 15027.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.63721025605974 23.518706800064933 18.55984552685765 24.284237417017675 2 13.133634647095512 21.93253761438104 41.1464162743607 23.787411464162744 3 15.393060670010167 31.09241902548636 35.15631007407534 18.35821023042813 4 9.877139171073878 19.99606982049332 28.18921251164102 41.93757849679178 5 13.87566962569312 38.11633331339764 28.59760942559572 19.410387635313516 6 26.029749750091845 36.808267047153606 15.507975701238005 21.65400750151654 7 22.185008928342576 34.47835410917355 15.517801150004699 27.818835812479175 8 28.387857454098064 27.568073272216193 24.363695394000494 19.680373879685245 9 20.87523388839999 10.915219192946182 14.642054629495144 53.56749228915868 10 15.92534367711012 26.070333125432533 27.255367685380588 30.74895551207676 11 30.58405885016618 19.955913638577275 21.446818690566715 28.013208820689833 12 19.148518066009927 27.87052621686047 21.37419580837812 31.606759908751485 13 36.722828362225854 16.30255547106619 22.765564792426716 24.209051374281245 14 23.54903753321429 25.203984860265034 23.062464222550684 28.184513383969996 15 29.37510145843835 25.245849815879634 18.55685517288518 26.822193552796836 16 28.173833548354022 22.06112283519732 18.10787488358979 31.657168732858864 17 18.727305349316065 33.05152807087993 19.06606973505464 29.155096844749366 18 25.776424049281033 16.212417658467402 21.994053467529028 36.01710482472254 19 27.194279025657238 24.09029160223166 21.702280358500722 27.01314901361038 20 26.139965653648662 18.90843536136292 24.782772143571165 30.168826841417257 21 28.53054005792743 20.045624257751424 27.88804114727066 23.535794537050485 22 19.625693121331476 37.643430192322484 22.189280862588966 20.54159582375708 23 24.718265936450706 17.831907931273122 26.609878420751347 30.83994771152482 24 23.628495510197105 26.926855941833345 28.3904206146459 21.05422793332365 25 23.722905257042285 30.06373725895611 23.094503729398596 23.11885375460301 26 28.760370120383104 21.20460001879651 26.726502225677745 23.30852763514264 27 24.460241107968866 32.59528549336569 25.338977982450896 17.605495416214552 28 18.062592380578078 18.909289748212196 34.56208402040276 28.466033850806973 29 19.03659338875456 25.82384251941594 26.524439735823584 28.61512435600591 30 22.579735652708834 22.207650179848432 30.722042326324512 24.49057184111822 31 25.930640875575644 17.216322206368602 32.922515656639014 23.930521261416747 32 20.794067137718617 31.997214698871357 27.779106823987764 19.429611339422266 33 22.88133421050383 20.407884281845135 32.59699426706424 24.11378724058679 34 17.197525695684494 29.547260408567794 30.862588963030678 22.392624932717037 35 19.56033252736174 24.995087275616655 38.07318677750912 17.371393419512486 36 28.671513888058236 20.063139188161617 23.31493553651222 27.950411387267927 37 22.199960698204933 23.481968165545997 29.44302521295592 24.87504592329315 38 21.13026836290936 18.062592380578078 29.841169484719295 30.965969771793272 39 29.463957690763227 19.309569987098758 26.09980947173261 25.126662850405406 40 18.40648308741232 19.13271190929829 40.44154712370667 22.019257879582717 41 24.39103577317738 21.825312064796698 24.424784053723844 29.358868108302076 42 22.839469254889227 18.254402228240902 36.74803277427954 22.15809574259033 43 24.76782037370881 18.515844604119852 31.837444358056437 24.8788906641149 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 5.0 2 10.0 3 44.5 4 79.0 5 79.0 6 138.0 7 197.0 8 239.5 9 282.0 10 407.0 11 532.0 12 532.0 13 921.0 14 1310.0 15 3419.5 16 5529.0 17 5418.5 18 5308.0 19 5308.0 20 4468.0 21 3628.0 22 2810.0 23 1992.0 24 1894.5 25 1797.0 26 1797.0 27 1759.0 28 1721.0 29 1657.0 30 1593.0 31 1558.5 32 1524.0 33 1524.0 34 1898.0 35 2272.0 36 2240.0 37 2208.0 38 2397.0 39 2586.0 40 2586.0 41 3363.0 42 4140.0 43 5649.0 44 7158.0 45 9962.0 46 12766.0 47 12766.0 48 17931.5 49 23097.0 50 33866.0 51 44635.0 52 33756.5 53 22878.0 54 22878.0 55 31517.0 56 40156.0 57 33061.0 58 25966.0 59 17289.5 60 8613.0 61 8613.0 62 7037.0 63 5461.0 64 4260.0 65 3059.0 66 2514.5 67 1970.0 68 1970.0 69 1529.0 70 1088.0 71 744.5 72 401.0 73 242.5 74 84.0 75 84.0 76 54.0 77 24.0 78 17.0 79 10.0 80 9.0 81 8.0 82 8.0 83 5.0 84 2.0 85 2.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 234086.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.71967567475201 #Duplication Level Percentage of deduplicated Percentage of total 1 73.69857672060832 14.533120306212247 2 10.00411602868222 3.9455584699640305 3 4.272004505968241 2.5272763001631877 4 2.432789584281103 1.9189528634775255 5 1.5055999653387058 1.4844971506198577 6 1.0874981044604752 1.2867065950120895 7 0.8600333614956348 1.1871705270712474 8 0.5849093390524468 0.9227377972198253 9 0.5784103463963085 1.0265457994070555 >10 4.007712137951951 16.203019403125346 >50 0.5242520742618227 7.2601522517365416 >100 0.3899395593682979 15.161094640431294 >500 0.02166330885379433 3.2261647428722777 >1k 0.02166330885379433 8.584451868116846 >5k 0.004332661770758865 5.3100142682603835 >10k+ 0.0064989926561382985 15.422537016310244 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG 13150 5.617593534000324 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 12077 5.159214989362884 TruSeq Adapter, Index 11 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT 10875 4.645728492947036 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC 7296 3.116803226164743 No Hit CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC 5134 2.19321104209564 RNA PCR Primer, Index 11 (95% over 22bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 3819 1.6314516886956076 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 3562 1.521662978563434 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 3196 1.3653101851456302 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 1762 0.7527148142135797 No Hit CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG 1625 0.6941893150380629 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1399 0.5976436010696923 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 1291 0.5515067112087011 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA 1160 0.49554437258101725 No Hit TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT 1149 0.4908452449099904 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT 1132 0.4835829566911306 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 993 0.42420307066633633 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 908 0.3878916295720376 No Hit ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 828 0.353716155600933 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG 780 0.3332108712182702 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 756 0.3229582290269388 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 725 0.30971523286313574 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC 712 0.3041617183428313 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG 671 0.28664678793264015 No Hit GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG 628 0.2682774706731714 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG 551 0.23538357697598317 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 489 0.2088975846483771 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 480 0.2050528438266278 No Hit GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG 478 0.2041984569773502 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG 477 0.2037712635527114 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG 465 0.1986449424570457 No Hit GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG 449 0.19180984766282477 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 447 0.19095546081354717 No Hit GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG 444 0.18967388053963075 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 440 0.1879651068410755 No Hit GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG 439 0.1875379134164367 No Hit GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC 424 0.18113001204685458 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG 414 0.1768580778004665 No Hit AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT 413 0.17643088437582768 No Hit GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA 389 0.16617824218449628 No Hit GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG 385 0.16446946848594107 No Hit ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT 383 0.16361508163666344 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA 374 0.15977034081491417 No Hit GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG 369 0.15763437369172012 No Hit TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 363 0.15507121314388728 TruSeq Adapter, Index 11 (95% over 23bp) CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT 363 0.15507121314388728 No Hit TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG 357 0.15250805259605446 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC 354 0.15122647232213804 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 350 0.14951769862358277 No Hit GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA 338 0.1443913775279171 No Hit ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA 338 0.1443913775279171 No Hit GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG 324 0.13841066958297377 No Hit ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG 298 0.12730364054236476 No Hit ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC 292 0.12474047999453193 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 290 0.12388609314525431 No Hit GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 286 0.12217731944669907 No Hit GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG 285 0.12175012602206026 No Hit CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA 282 0.12046854574814383 No Hit TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA 282 0.12046854574814383 No Hit TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA 273 0.11662380492639458 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC 267 0.11406064437856171 No Hit GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA 258 0.11021590355681245 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT 258 0.11021590355681245 No Hit GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC 254 0.10850712985825722 No Hit CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG 253 0.10807993643361842 No Hit CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG 252 0.10765274300897962 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC 248 0.10594396931042438 No Hit GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG 245 0.10466238903650794 No Hit GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG 244 0.10423519561186913 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC 243 0.10380800218723032 No Hit AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA 243 0.10380800218723032 No Hit GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC 242 0.10338080876259152 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA 242 0.10338080876259152 No Hit CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG 242 0.10338080876259152 No Hit CTGATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT 238 0.1016720350640363 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC 238 0.1016720350640363 No Hit ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC 235 0.10039045479011988 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.27193424638808E-4 0.0 10 0.0 0.0 0.0 4.27193424638808E-4 0.0 11 0.0 0.0 0.0 4.27193424638808E-4 0.0 12 0.0 0.0 0.0 4.27193424638808E-4 0.0 13 0.0 0.0 0.0 4.27193424638808E-4 0.0 14 0.0 0.0 0.0 4.27193424638808E-4 0.0 15 0.0 0.0 0.0 4.27193424638808E-4 0.0 16 0.0 0.0 0.0 8.54386849277616E-4 0.0 17 0.0 0.0 0.0 8.54386849277616E-4 0.0 18 0.0 0.0 0.0 0.0012815802739164239 0.0 19 0.0 0.0 0.0 0.0012815802739164239 0.0 20 0.0 0.0 0.0 0.001708773698555232 0.0 21 0.0 0.0 0.0 0.0029903539724716556 0.0 22 0.0 0.0 0.0 0.003417547397110464 0.0 23 0.0 0.0 0.0 0.00427193424638808 0.0 24 0.0 0.0 0.0 0.0051263210956656955 0.0 25 0.0 0.0 0.0 0.005553514520304503 0.0 26 0.0 0.0 0.0 0.005980707944943311 0.0 27 0.0 0.0 0.0 0.005980707944943311 0.0 28 0.0 0.0 0.0 0.017514930410191126 0.0 29 0.0 0.0 0.0 0.05040882410737934 0.0 30 0.0 0.0 0.0 0.08543868492776159 0.0 31 0.0 0.0 0.0 0.13712908930905734 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTATT 30 8.283709E-6 37.0 10 TAGGACC 35 5.605798E-7 37.0 4 GATACAC 40 3.8109647E-8 37.0 3 CTTAGGA 35 5.605798E-7 37.0 2 TGATACA 40 3.8109647E-8 37.0 2 ATTACGG 45 2.6011548E-9 37.0 2 ACTCAGA 20 0.001838943 37.0 1 TCAGAGT 20 0.001838943 37.0 3 TTAGGAC 35 5.605798E-7 37.0 3 TTACGGG 45 2.6011548E-9 37.0 3 TAGCAAC 25 1.230624E-4 36.999996 1 AGCAACG 25 1.230624E-4 36.999996 2 CTTATAC 1210 0.0 36.23554 1 CGTCTTC 605 0.0 33.636364 37 GATTACG 50 7.2795956E-9 33.3 1 GACCGTT 40 1.592869E-6 32.375 7 TTATACA 1350 0.0 32.34074 2 TTACTTT 35 2.3802491E-5 31.714285 3 ATTACTT 35 2.3802491E-5 31.714285 2 ACAGACA 30 3.5908018E-4 30.833334 17 >>END_MODULE