FastQCFastQC Report
Fri 10 Feb 2017
ERR1630908.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630908.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310470
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG52751.699036943988147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51191.6487905433697296No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT49471.5933906657648083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44531.434277063806487No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT37301.2014043224788225No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC34911.124424259992914No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19220.6190614230038329No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC18900.6087544690308242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15900.512126775533868No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13470.4338583438013335No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC11410.3675073276000902Illumina PCR Primer Index 7 (95% over 21bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10900.3510806197056076No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10790.3475376042773859No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG8520.2744226495313557No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8210.26443778787000355No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8150.2625052340000644No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8070.25992849550681224No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8030.2586401262601862No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7700.24801107997552096No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6510.20968209488839504No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6080.1958321254871646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.1906786485006603No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5700.18359261764421683No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5330.17167520211292556No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5250.1690984636196734No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5240.16877637130801687No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4960.15975778658163428No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4900.15782523271169516No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.15718104808838212No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4580.1475182787386865No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4550.14655200180371694No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA4540.1462299094920604No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4520.14558572486874738No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT4490.14461944793377782No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4490.14461944793377782No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4450.14333107868715173No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4400.14172061712886913No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4070.13109157084420395No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4000.1288369246626083No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3980.12819274003929526No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3880.12497181692273004No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3820.12303926305279092No Hit
ACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT3790.12207298611782136RNA PCR Primer, Index 41 (95% over 21bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3760.1211067091828518No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3730.12014043224788223No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3550.11434277063806486No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3490.11241021676812574No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3310.10661255515830836No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3260.10500209360002576No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3230.1040358166650562No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3200.10306953973008663No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3180.10242535510677361No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3180.10242535510677361No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3130.10081489354849099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG850.036.9999961
TTACGGG900.034.9444433
CTTATAC5700.034.0789451
CGTCTTC2000.032.37537
TTATACA6200.031.0322592
ATTACTT303.5934823E-430.8333322
TATACAC6550.029.6564883
GCAACCG250.005490241629.69
CGTTATT250.005490241629.610
AATTGCA250.005490241629.617
TCAGAGT502.7099668E-729.63
CGAGAGA250.005490241629.636
GAATTGC250.005490241629.616
CCCATAG250.005490241629.61
ATCTGTT250.005490241629.623
GTACATG453.9958795E-628.7777771
TGTGCAA453.9958795E-628.77777710
TACATGC453.9958795E-628.7777772
CTGTGCA453.9958795E-628.7777779
TCTATAG453.9958795E-628.7777771
Produced by FastQC (version 0.11.5)