FastQCFastQC Report
Fri 10 Feb 2017
ERR1630907.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630907.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814794
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT177932.1837421483221524No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG148241.819355567174034No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT134761.6539149772826014TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC77780.9545971128898838No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC53240.6534166918264003RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC37940.46563916769146557No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA34820.42734728041689063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32120.3942100702754316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30420.3733459009271055No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG18430.22619214181744096No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA15370.18863663699045405No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT11920.1462946462541452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11780.1445764205431066No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11280.13843990014654012No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT9720.11929395650925265No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA9690.11892576528545866No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC8270.10149804735920982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGA200.001841621237.01
CTTATAC22750.033.909891
CGTCTTC6500.033.01538537
TTATACA24900.031.2791182
TATACAC26050.030.2533593
GGTATCA4900.026.428571
CCGTCTT21900.025.76484137
GCCGTCT22800.024.74780836
GTATCAA14500.024.4965521
GCTTTAT7650.022.7320251
GAATCGT502.7011664E-422.26
GCACCGC753.738005E-722.210
GATTACG753.738005E-722.21
TGCCGTC26000.021.77307735
ATGCCGT27300.020.73626334
GCGTCAA450.003824866420.55555526
TTATTGA8500.019.8058834
TATGCCG29650.019.27993233
CTTTATT9500.019.084212
ATTGAGC8400.018.9404756
Produced by FastQC (version 0.11.5)