FastQCFastQC Report
Fri 10 Feb 2017
ERR1630906.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630906.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731297
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT113441.551216537193507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103051.4091401988521763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT99951.3667497610409998No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG94381.29058371632866No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT72290.9885176610870824RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC53810.7358159543933586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33270.45494511805736926No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA32560.44523634036513204No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC29080.3976496553383919RNA PCR Primer, Index 29 (95% over 21bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC26520.36264335830722677RNA PCR Primer, Index 29 (95% over 24bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA21670.2963228346349021No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT19020.26008584747373503No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA16920.23136974444035735No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14890.20361084484142558No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14620.1999187744514199No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC14370.19650019075697014No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG14290.19540624397474624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14240.19472252723585629No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG13370.18282585597917125No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC13330.1822788825880593No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG12790.1748947418080479No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11410.1560241598146854No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG10910.14918699242578598No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG9690.13250430399687133No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9480.12963269369353356No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9220.12607736665130584No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.1253936499124159No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA9020.12334249969574605No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8960.12252203960907812No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8590.11746253574129253No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8550.11691556235018055No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8380.11459092543795477No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8070.1103518816568371No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8070.1103518816568371No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTAT8040.10994165161350314No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG7790.1065230679190534No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7720.10556586448460749No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7710.1054291211368295No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7590.10378820096349363No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7480.10228402413793576No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7380.10091659066015586No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT7330.10023287392126592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG1250.035.521
AGCAACG451.0790609E-732.888892
CTTATAC14700.031.5884361
CGTCTTC3550.030.7464837
TTATACA15100.030.629142
CGCGGTC250.005495053329.59999832
ATTACGG1450.029.3448282
TATACAC16350.028.740063
GGTATCA11700.027.1965831
TTACGGG1700.026.1176473
CTAAGTC509.085186E-625.8999984
GTTGTAT809.767973E-1025.43751
GTATCAA35400.025.2937851
CCGTCTT11150.025.05381237
TGGACGT601.3362951E-624.66666826
GTTATGC851.9736035E-923.9411771
TTGTATC701.9209256E-723.7857132
GCCGTCT11800.023.67372936
TAAGTCT551.9014313E-523.5454545
CGTTATT551.9014313E-523.54545410
Produced by FastQC (version 0.11.5)