##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630906.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	731297
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22684764192934	31.0	31.0	34.0	28.0	34.0
2	31.169926855983274	31.0	31.0	34.0	28.0	34.0
3	30.57906568740197	31.0	30.0	34.0	26.0	34.0
4	34.82520508083583	37.0	35.0	37.0	32.0	37.0
5	35.06275699202923	35.0	35.0	37.0	32.0	37.0
6	35.29655803319308	37.0	35.0	37.0	33.0	37.0
7	35.303332298642005	37.0	35.0	37.0	33.0	37.0
8	35.32384790310913	37.0	35.0	37.0	32.0	37.0
9	37.07931524401167	39.0	37.0	39.0	33.0	39.0
10	36.89575507625493	39.0	37.0	39.0	33.0	39.0
11	36.96347448437502	39.0	37.0	39.0	33.0	39.0
12	36.86192613944813	39.0	37.0	39.0	33.0	39.0
13	36.94191279329739	39.0	37.0	39.0	33.0	39.0
14	37.89698439895145	39.0	37.0	41.0	33.0	41.0
15	37.89312413424368	39.0	37.0	41.0	33.0	41.0
16	37.93498811016591	39.0	37.0	41.0	33.0	41.0
17	37.96316544440904	40.0	37.0	41.0	33.0	41.0
18	38.05281164834534	40.0	37.0	41.0	33.0	41.0
19	38.06192422504126	40.0	37.0	41.0	33.0	41.0
20	38.05002208405067	40.0	37.0	41.0	33.0	41.0
21	37.98702305629587	40.0	37.0	41.0	33.0	41.0
22	37.8777870003569	40.0	37.0	41.0	33.0	41.0
23	37.876400422810434	40.0	37.0	41.0	33.0	41.0
24	37.81087574542217	40.0	37.0	41.0	33.0	41.0
25	37.77174800388898	39.0	37.0	41.0	33.0	41.0
26	37.62010920323753	39.0	37.0	41.0	32.0	41.0
27	37.50532820454617	39.0	36.0	41.0	32.0	41.0
28	37.475854543366104	39.0	36.0	41.0	32.0	41.0
29	37.37138809539763	39.0	36.0	41.0	32.0	41.0
30	37.187097718163756	39.0	36.0	40.0	31.0	41.0
31	37.071297981531444	39.0	36.0	40.0	31.0	41.0
32	36.91946910762659	39.0	35.0	40.0	31.0	41.0
33	36.863386558402404	39.0	35.0	40.0	31.0	41.0
34	36.771094370686605	39.0	35.0	40.0	31.0	41.0
35	36.674516646451444	39.0	35.0	40.0	30.0	41.0
36	36.566347188625144	39.0	35.0	40.0	30.0	41.0
37	36.45969011222527	39.0	35.0	40.0	30.0	41.0
38	36.359903021617754	39.0	35.0	40.0	30.0	41.0
39	36.23136153983949	38.0	35.0	40.0	30.0	41.0
40	36.069635182422466	38.0	35.0	40.0	30.0	41.0
41	35.924174446223624	38.0	35.0	40.0	29.0	41.0
42	35.746305536601405	38.0	35.0	40.0	28.0	41.0
43	34.99320932534935	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	5.0
10	10.0
11	9.0
12	9.0
13	5.0
14	8.0
15	9.0
16	12.0
17	14.0
18	31.0
19	65.0
20	133.0
21	264.0
22	534.0
23	931.0
24	1582.0
25	2611.0
26	4132.0
27	6041.0
28	8695.0
29	12015.0
30	15761.0
31	20023.0
32	25648.0
33	32686.0
34	43006.0
35	56752.0
36	78207.0
37	115583.0
38	172381.0
39	134129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.90458459422096	21.115771020529277	15.97039233033911	22.009252054910657
2	14.939757718136407	23.813580528841225	39.8810606361027	21.365601116919663
3	15.976545780989118	29.131529323927214	33.694107865887595	21.197817029196074
4	11.490406770436635	18.52489481017972	37.956398016127515	32.02830040325613
5	13.1804178056248	38.45797261577717	32.80773748559067	15.553872093007357
6	26.64567200467115	40.998253787448874	16.34438538651191	16.01168882136806
7	24.700224395833704	32.57595751110698	21.107703163010378	21.61611493004894
8	26.633091616675582	31.678647662987814	21.7412350932658	19.947025627070808
9	25.344559050563586	13.086748612396878	19.352192064236558	42.21650027280298
10	16.007313034239168	28.11867134693565	31.248179604182706	24.625836014642477
11	33.024475691818786	23.128222869777943	23.491276458128503	20.35602498027477
12	22.383655341126794	27.298211260267717	27.794042639310707	22.524090759294786
13	31.656221753952224	20.805226877725467	25.509745014679396	22.02880635364291
14	22.670132654721677	21.965767670317256	28.931473806128018	26.43262586883305
15	25.165015034931088	27.299578693745495	24.710753633612608	22.824652637710805
16	22.970147559746586	26.185120409354884	24.11236474373613	26.732367287162397
17	21.17087858968381	27.777633437577347	25.529572800107207	25.521915172631637
18	24.436446477969966	22.762844644515155	28.630911927711995	24.169796949802887
19	25.912454173885575	24.12453490168837	29.241607718888496	20.72140320553756
20	25.132743604855484	23.007478493689977	28.279891753965895	23.57988614748864
21	24.63048528846693	23.818913519404564	28.747280516671065	22.803320675457442
22	21.97862154500839	28.51932935592516	28.68273765651985	20.8193114425466
23	24.0914430115261	23.426596854629516	29.02514299935594	23.45681713448845
24	22.758879087429595	25.27030741272014	29.265127574706312	22.705685925143957
25	22.80796994928189	26.54174706035988	29.528358519178937	21.121924471179288
26	23.493054121649617	25.616131339250675	29.26786244166187	21.62295209743784
27	22.875794649779774	27.148614037798595	29.439201856427687	20.536389455993938
28	21.257573872175055	24.79416707575718	29.903445522133964	24.044813529933805
29	21.963716520100586	25.862816338642165	29.51878648483448	22.654680656422766
30	24.06122273166716	24.753827788162674	30.59235850823947	20.592590971930694
31	23.71239045148551	24.192769832229587	27.384906542758962	24.70993317352594
32	20.957011993759032	26.168847950969305	30.937635461378893	21.93650459389277
33	22.254432877476592	25.50495899750717	30.912064455344407	21.32854366967183
34	20.547465667163955	25.739610582294198	29.387786357663163	24.32513739287868
35	20.902588141343394	25.267846032460135	33.193353726324595	20.63621209987187
36	22.903006575987593	26.59958949646997	27.744268060719516	22.75313586682292
37	22.627742216910505	25.73044877799307	29.213575332594004	22.42823367250242
38	22.03783141459626	24.385577952596552	29.383684057229825	24.192906575577364
39	23.3937784511628	24.1454566338984	29.18226110595285	23.278503808985953
40	21.28820438207732	24.801961446580528	32.258712944262044	21.651121227080104
41	23.128496356473498	24.12576559181837	28.135764265407897	24.60997378630023
42	22.343589540227843	24.277140477808608	30.711872194197433	22.66739778776612
43	23.23023340722032	23.52409486159522	30.135909213356545	23.109762517827914
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	32.0
2	51.0
3	233.0
4	415.0
5	415.0
6	605.5
7	796.0
8	854.0
9	912.0
10	1365.0
11	1818.0
12	1818.0
13	2889.0
14	3960.0
15	9628.5
16	15297.0
17	15523.0
18	15749.0
19	15749.0
20	12793.5
21	9838.0
22	8652.5
23	7467.0
24	8636.0
25	9805.0
26	9805.0
27	11144.5
28	12484.0
29	14006.5
30	15529.0
31	17303.0
32	19077.0
33	19077.0
34	22309.0
35	25541.0
36	27805.5
37	30070.0
38	31924.5
39	33779.0
40	33779.0
41	36458.0
42	39137.0
43	42073.5
44	45010.0
45	49012.0
46	53014.0
47	53014.0
48	58613.5
49	64213.0
50	72251.5
51	80290.0
52	70440.5
53	60591.0
54	60591.0
55	64718.5
56	68846.0
57	59337.0
58	49828.0
59	38687.5
60	27547.0
61	27547.0
62	23023.5
63	18500.0
64	14373.0
65	10246.0
66	8134.5
67	6023.0
68	6023.0
69	4577.5
70	3132.0
71	2260.5
72	1389.0
73	924.5
74	460.0
75	460.0
76	336.0
77	212.0
78	163.0
79	114.0
80	82.5
81	51.0
82	51.0
83	42.0
84	33.0
85	31.5
86	30.0
87	24.0
88	18.0
89	18.0
90	14.0
91	10.0
92	5.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	731297.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.748212702193516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.645689747052	23.063708099869658
2	13.43106057551427	8.528243359349453
3	4.82799982926587	4.598410965170604
4	2.3151574160687733	2.940084403376486
5	1.3144565937447739	2.0865823763004925
6	0.8429850812987232	1.6057961799508655
7	0.5848118305370194	1.299671127065391
8	0.47688445668050233	1.2112183332050064
9	0.33716356033675715	0.9633906386100202
>10	2.619242208942669	16.89547627312739
>50	0.35676157223808025	7.927206681083927
>100	0.22184083216134423	13.568236608895178
>500	0.01600021596485329	3.6397328766991146
>1k	0.007351450578446105	4.300791071460015
>5k	0.0017297530772814366	4.399229020711015
>10k+	8.648765386407183E-4	2.972221985125387
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	11344	1.551216537193507	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10305	1.4091401988521763	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9995	1.3667497610409998	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	9438	1.29058371632866	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	7229	0.9885176610870824	RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	5381	0.7358159543933586	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3327	0.45494511805736926	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	3256	0.44523634036513204	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	2908	0.3976496553383919	RNA PCR Primer, Index 29 (95% over 21bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC	2652	0.36264335830722677	RNA PCR Primer, Index 29 (95% over 24bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	2167	0.2963228346349021	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1902	0.26008584747373503	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1692	0.23136974444035735	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1489	0.20361084484142558	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1462	0.1999187744514199	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1437	0.19650019075697014	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1429	0.19540624397474624	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1424	0.19472252723585629	No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG	1337	0.18282585597917125	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	1333	0.1822788825880593	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	1279	0.1748947418080479	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1141	0.1560241598146854	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	1091	0.14918699242578598	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	969	0.13250430399687133	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	948	0.12963269369353356	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	922	0.12607736665130584	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	917	0.1253936499124159	No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA	902	0.12334249969574605	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	896	0.12252203960907812	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	859	0.11746253574129253	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	855	0.11691556235018055	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	838	0.11459092543795477	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	807	0.1103518816568371	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	807	0.1103518816568371	No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTAT	804	0.10994165161350314	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	779	0.1065230679190534	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	772	0.10556586448460749	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	771	0.1054291211368295	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	759	0.10378820096349363	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	748	0.10228402413793576	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	738	0.10091659066015586	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	733	0.10023287392126592	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.3674334777798896E-4	0.0
10	0.0	0.0	0.0	1.3674334777798896E-4	0.0
11	0.0	0.0	0.0	1.3674334777798896E-4	0.0
12	0.0	0.0	0.0	1.3674334777798896E-4	0.0
13	0.0	0.0	0.0	1.3674334777798896E-4	0.0
14	0.0	0.0	0.0	1.3674334777798896E-4	0.0
15	0.0	0.0	0.0	1.3674334777798896E-4	0.0
16	0.0	0.0	0.0	1.3674334777798896E-4	0.0
17	0.0	0.0	0.0	1.3674334777798896E-4	0.0
18	0.0	0.0	0.0	1.3674334777798896E-4	0.0
19	0.0	0.0	0.0	1.3674334777798896E-4	0.0
20	0.0	0.0	0.0	1.3674334777798896E-4	0.0
21	0.0	0.0	0.0	9.572034344459228E-4	0.0
22	0.0	0.0	0.0	0.0016409201733358677	0.0
23	0.0	0.0	0.0	0.0027348669555597796	0.0
24	0.0	0.0	0.0	0.0031450969988937464	0.0
25	0.0	0.0	0.0	0.003418583694449724	0.0
26	0.0	0.0	0.0	0.00396555708556168	0.0
27	0.0	0.0	0.0	0.006563680693343471	0.0
28	0.0	0.0	0.0	0.020921732210032313	0.0
29	0.0	0.0	0.0	0.05250944554674777	0.0
30	0.0	0.0	0.0	0.09079758292458467	0.0
31	0.0	0.0	0.0	0.16354504394247482	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	125	0.0	35.52	1
AGCAACG	45	1.0790609E-7	32.88889	2
CTTATAC	1470	0.0	31.588436	1
CGTCTTC	355	0.0	30.74648	37
TTATACA	1510	0.0	30.62914	2
CGCGGTC	25	0.0054950533	29.599998	32
ATTACGG	145	0.0	29.344828	2
TATACAC	1635	0.0	28.74006	3
GGTATCA	1170	0.0	27.196583	1
TTACGGG	170	0.0	26.117647	3
CTAAGTC	50	9.085186E-6	25.899998	4
GTTGTAT	80	9.767973E-10	25.4375	1
GTATCAA	3540	0.0	25.293785	1
CCGTCTT	1115	0.0	25.053812	37
TGGACGT	60	1.3362951E-6	24.666668	26
GTTATGC	85	1.9736035E-9	23.941177	1
TTGTATC	70	1.9209256E-7	23.785713	2
GCCGTCT	1180	0.0	23.673729	36
TAAGTCT	55	1.9014313E-5	23.545454	5
CGTTATT	55	1.9014313E-5	23.545454	10
>>END_MODULE