FastQCFastQC Report
Fri 10 Feb 2017
ERR1630905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144777
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT1632211.273890189740083No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG1459410.080330439227225No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT138709.58025100672068TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC74165.122360595951014No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC61684.260345220580617RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC41472.864405257741216No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG16071.1099829392790292No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA14901.0291689978380545No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT12690.8765204417828798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12320.8509638961989819No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10580.73077906020984No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT9360.646511531527798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4740.3274000704531797No Hit
TCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCT4620.3191114610746182TruSeq Adapter, Index 8 (95% over 24bp)
ATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT4170.28802917590501254TruSeq Adapter, Index 8 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3330.23000891025508194No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA2590.17889581908728597No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2380.16439075267480333No Hit
CTGATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT2250.15541142584802836No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2220.15333927350338797No Hit
TCGCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT2090.144359946676613TruSeq Adapter, Index 8 (95% over 22bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2070.14297851178018608No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2000.13814348964269185No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1730.11949411854092847No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.11534981385164772No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1630.11258694405879388No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1550.10706120447308619No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1550.10706120447308619No Hit
CTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA1550.10706120447308619No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1460.10084474743916505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACA452.586603E-937.017
CAGACAC452.586603E-937.018
AGACAGA452.586603E-937.015
CTTATAC17850.036.585431
CGTCTTC7650.034.09803837
TTATACA19650.033.3282472
GACAGAC507.237759E-933.316
GATACAC451.0689109E-732.888893
TATACAC21300.031.007043
GTCTTCT551.8320861E-830.27272837
ACTGTGC405.9048747E-527.7500028
CCGTACA601.3239624E-624.66666817
GGCACGG451.315423E-424.66666620
TGATACA451.315423E-424.6666662
CCGTCTT22450.024.2271737
GCCGTCT22750.023.9890136
GACACAT701.8988794E-723.78571520
ACAGCCC551.8878072E-523.54545411
CGCCTCA400.001923087323.12500227
TACACAA652.6560465E-622.7692320
Produced by FastQC (version 0.11.5)