##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630905.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	144777
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.991048301871153	31.0	30.0	34.0	28.0	34.0
2	31.157096776421668	31.0	31.0	34.0	28.0	34.0
3	30.66078175400789	31.0	30.0	34.0	26.0	34.0
4	34.71344895943417	35.0	35.0	37.0	32.0	37.0
5	34.87566395214709	35.0	35.0	37.0	32.0	37.0
6	35.04805321287221	36.0	35.0	37.0	32.0	37.0
7	34.95900591944853	36.0	35.0	37.0	32.0	37.0
8	35.07154451328595	36.0	35.0	37.0	32.0	37.0
9	36.9048122284617	39.0	37.0	39.0	33.0	39.0
10	36.72690413532536	39.0	35.0	39.0	32.0	39.0
11	36.70109893146011	39.0	35.0	39.0	32.0	39.0
12	36.55720867264828	38.0	35.0	39.0	32.0	39.0
13	36.74107075018822	39.0	37.0	39.0	32.0	39.0
14	37.38658074141611	39.0	36.0	40.0	32.0	41.0
15	37.38544796480104	39.0	36.0	40.0	32.0	41.0
16	37.42588256421946	39.0	36.0	40.0	32.0	41.0
17	37.27317184359394	39.0	36.0	40.0	31.0	41.0
18	37.57018034632573	39.0	37.0	40.0	32.0	41.0
19	37.465329437686925	39.0	36.0	40.0	32.0	41.0
20	37.571858789724885	39.0	37.0	41.0	32.0	41.0
21	37.58786962017447	39.0	37.0	41.0	32.0	41.0
22	37.22981550936958	39.0	36.0	40.0	31.0	41.0
23	37.35444165855073	39.0	36.0	40.0	31.0	41.0
24	37.19864343093171	39.0	36.0	40.0	31.0	41.0
25	36.99224324305656	39.0	36.0	40.0	31.0	41.0
26	36.81611720093661	39.0	35.0	40.0	30.0	41.0
27	36.693749697811114	39.0	35.0	40.0	30.0	41.0
28	36.83085711128149	39.0	35.0	40.0	30.0	41.0
29	36.83221782465447	39.0	35.0	40.0	30.0	41.0
30	36.696560917825344	39.0	35.0	40.0	30.0	41.0
31	36.58843600848201	38.0	35.0	40.0	30.0	41.0
32	36.31263253141038	38.0	35.0	40.0	30.0	41.0
33	35.86629091637484	38.0	35.0	40.0	29.0	41.0
34	35.8768588933325	38.0	35.0	40.0	29.0	41.0
35	36.03296794380323	38.0	35.0	40.0	30.0	41.0
36	35.884097612189784	38.0	35.0	40.0	29.0	41.0
37	35.83707356831541	38.0	35.0	40.0	29.0	41.0
38	35.77437023836659	38.0	34.0	40.0	28.0	41.0
39	35.353757848277006	38.0	33.0	40.0	26.0	41.0
40	34.868508119383606	38.0	33.0	40.0	25.0	41.0
41	34.99057861400637	38.0	33.0	40.0	26.0	41.0
42	34.911539816407306	38.0	33.0	40.0	26.0	41.0
43	33.608183620326436	37.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	18.0
11	23.0
12	14.0
13	10.0
14	9.0
15	10.0
16	16.0
17	16.0
18	15.0
19	21.0
20	49.0
21	88.0
22	142.0
23	270.0
24	494.0
25	797.0
26	1217.0
27	1754.0
28	2435.0
29	3196.0
30	4069.0
31	5107.0
32	6290.0
33	7678.0
34	9525.0
35	12180.0
36	15763.0
37	21667.0
38	30949.0
39	20941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.143551807262202	26.504900640295077	20.416917051741645	31.93463050070108
2	8.075868404511766	17.285894858990034	47.499257478743175	27.13897925775503
3	9.577488136927826	31.726724548788827	42.21388756501378	16.481899749269566
4	6.496197600447585	23.722000041443046	20.351298894161367	49.430503463948
5	11.747722359214515	36.32137701430476	31.732250288374537	20.19865033810619
6	23.8325148331572	37.208258217810844	14.025017785974292	24.93420916305767
7	17.359801625948872	37.64686379742639	11.65861980839498	33.33471476822976
8	27.04849527203907	28.32770398613039	25.933677310622542	18.690123431207994
9	13.945585279429743	10.77795506192282	10.549327586564164	64.72713207208326
10	12.798994315395401	26.888248824053544	26.169211960463336	34.14354490008772
11	22.77778928973525	17.1353184552795	24.104657507753302	35.98223474723195
12	12.848035254218557	28.650959751894295	15.287649281308497	43.213355712578654
13	36.65637497668829	11.039046257347508	24.118471856717573	28.186106909246632
14	23.212250564661513	28.711742887337078	14.615581204196799	33.46042534380461
15	29.697396685937683	25.360381828605373	11.885865848857208	33.05635563659974
16	37.01416661486286	19.815302154347723	12.2857912513728	30.88473997941662
17	13.986337608874338	43.443364622833734	12.623552083549184	29.946745684742744
18	33.110231597560386	12.111730454423009	13.743895784551412	41.03414216346519
19	29.653190769252024	26.278345317281058	14.462932648141624	29.60553126532529
20	14.33998494235963	17.647830801853885	18.502248285293934	49.50993597049255
21	31.35788143144284	14.366922922839953	24.335357135456597	29.939838510260607
22	12.794159293257906	45.23992070563694	14.661859273227101	27.30406072787805
23	24.845797329686345	23.186003301629402	22.21899887413056	29.74920049455369
24	34.46403779605877	24.11639970437293	25.58970002141224	15.829862478156063
25	22.975334479924296	33.617908921997284	15.992181078486222	27.414575519592198
26	34.61806778701037	29.12548263881694	23.242642132382908	13.013807441789787
27	15.315968696685248	35.84823556227854	20.087444828943823	28.74835091209239
28	12.679500196854473	21.289983906283457	37.29390718138931	28.736608715472762
29	24.52323228137066	15.648203789275922	25.275423582475113	34.5531403468783
30	16.984742051568965	24.59921120067414	29.131008378402644	29.28503836935425
31	16.992339943499314	18.713607824447255	38.420467339425464	25.87358489262797
32	31.334397038203583	19.552829524026606	24.205502255192467	24.907271182577343
33	12.003287815053495	39.90481913563618	32.74346063255904	15.34843241675128
34	15.475524427222556	34.2450803649751	32.53348252830215	17.745912679500197
35	28.20199341055554	15.298010043031695	44.18519516221499	12.314801384197766
36	22.770882115253112	28.742134455058473	16.16278828819495	32.32419514149347
37	17.651975106543166	25.6594624836818	29.943982814949887	26.744579594825147
38	18.955358931321964	13.301145900246587	33.10954088011217	34.63395428831928
39	32.947222279782004	17.086968233904557	21.881928759402392	28.083880726911044
40	13.498000372987423	12.537903119970714	52.68792694972267	21.27616955731919
41	21.654682718940162	22.218308156682344	20.40033983298452	35.72666929139297
42	23.304806702722118	13.39577419065183	44.137535658288265	19.16188344833779
43	23.260600786036456	13.20582689239313	38.40803442535762	25.125537896212798
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	4.5
2	8.0
3	36.0
4	64.0
5	64.0
6	90.0
7	116.0
8	129.5
9	143.0
10	194.5
11	246.0
12	246.0
13	356.0
14	466.0
15	1181.0
16	1896.0
17	1859.0
18	1822.0
19	1822.0
20	1523.5
21	1225.0
22	976.0
23	727.0
24	780.0
25	833.0
26	833.0
27	987.0
28	1141.0
29	1135.5
30	1130.0
31	1157.0
32	1184.0
33	1184.0
34	1307.5
35	1431.0
36	1634.5
37	1838.0
38	1971.0
39	2104.0
40	2104.0
41	2351.0
42	2598.0
43	3089.0
44	3580.0
45	4254.5
46	4929.0
47	4929.0
48	7633.5
49	10338.0
50	25090.0
51	39842.0
52	24765.0
53	9688.0
54	9688.0
55	19920.0
56	30152.0
57	24254.0
58	18356.0
59	11126.0
60	3896.0
61	3896.0
62	3041.0
63	2186.0
64	1742.0
65	1298.0
66	1068.5
67	839.0
68	839.0
69	599.0
70	359.0
71	276.5
72	194.0
73	136.5
74	79.0
75	79.0
76	54.5
77	30.0
78	23.5
79	17.0
80	12.0
81	7.0
82	7.0
83	9.0
84	11.0
85	7.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	144777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.948928351879097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.57595115964378	16.368622087762557
2	11.275450797746798	4.94968123389765
3	4.437171539163546	2.921734805942933
4	2.464046322812097	2.1633270478045548
5	1.4475878780249867	1.5886501308909566
6	0.909462819020046	1.1977040552021385
7	0.6325329640935268	0.9718394496363372
8	0.5255373383264625	0.9227985108131818
9	0.46259873493407183	0.9138191839864067
>10	2.910910406898071	12.224317398481803
>50	0.23287283255184568	3.5392362046457655
>100	0.088114044749347	3.8127603141382957
>500	0.0031469301696195363	0.646511531527798
>1k	0.018881581017717218	7.4618205930500014
>5k	0.006293860339239073	9.382705816531631
>10k+	0.009440790508858609	30.934471635687988
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	16322	11.273890189740083	No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	14594	10.080330439227225	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	13870	9.58025100672068	TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	7416	5.122360595951014	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	6168	4.260345220580617	RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	4147	2.864405257741216	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	1607	1.1099829392790292	No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	1490	1.0291689978380545	No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT	1269	0.8765204417828798	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1232	0.8509638961989819	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1058	0.73077906020984	No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT	936	0.646511531527798	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	474	0.3274000704531797	No Hit
TCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCT	462	0.3191114610746182	TruSeq Adapter, Index 8 (95% over 24bp)
ATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT	417	0.28802917590501254	TruSeq Adapter, Index 8 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	333	0.23000891025508194	No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	259	0.17889581908728597	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	238	0.16439075267480333	No Hit
CTGATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	225	0.15541142584802836	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	222	0.15333927350338797	No Hit
TCGCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	209	0.144359946676613	TruSeq Adapter, Index 8 (95% over 22bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	207	0.14297851178018608	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	200	0.13814348964269185	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	173	0.11949411854092847	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	167	0.11534981385164772	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	163	0.11258694405879388	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	155	0.10706120447308619	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	155	0.10706120447308619	No Hit
CTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	155	0.10706120447308619	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	146	0.10084474743916505	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	6.907174482134593E-4	0.0
9	0.0	0.0	0.0	6.907174482134593E-4	0.0
10	0.0	0.0	0.0	6.907174482134593E-4	0.0
11	0.0	0.0	0.0	6.907174482134593E-4	0.0
12	0.0	0.0	0.0	0.0013814348964269185	0.0
13	0.0	0.0	0.0	0.0013814348964269185	0.0
14	0.0	0.0	0.0	0.0013814348964269185	0.0
15	0.0	0.0	0.0	0.0013814348964269185	0.0
16	0.0	0.0	0.0	0.0013814348964269185	0.0
17	0.0	0.0	0.0	0.0013814348964269185	0.0
18	0.0	0.0	0.0	0.0013814348964269185	0.0
19	0.0	0.0	0.0	0.0013814348964269185	0.0
20	0.0	0.0	0.0	0.002762869792853837	0.0
21	0.0	0.0	0.0	0.0034535872410672965	0.0
22	0.0	0.0	0.0	0.0034535872410672965	0.0
23	0.0	0.0	0.0	0.0034535872410672965	0.0
24	0.0	0.0	0.0	0.004835022137494215	0.0
25	0.0	0.0	0.0	0.004835022137494215	0.0
26	0.0	0.0	0.0	0.004835022137494215	0.0
27	0.0	0.0	0.0	0.005525739585707674	0.0
28	0.0	0.0	0.0	0.016577218757123025	0.0
29	0.0	0.0	0.0	0.04558735158208831	0.0
30	0.0	0.0	0.0	0.07874178909633436	0.0
31	0.0	0.0	0.0	0.1153498138516477	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGACA	45	2.586603E-9	37.0	17
CAGACAC	45	2.586603E-9	37.0	18
AGACAGA	45	2.586603E-9	37.0	15
CTTATAC	1785	0.0	36.58543	1
CGTCTTC	765	0.0	34.098038	37
TTATACA	1965	0.0	33.328247	2
GACAGAC	50	7.237759E-9	33.3	16
GATACAC	45	1.0689109E-7	32.88889	3
TATACAC	2130	0.0	31.00704	3
GTCTTCT	55	1.8320861E-8	30.272728	37
ACTGTGC	40	5.9048747E-5	27.750002	8
CCGTACA	60	1.3239624E-6	24.666668	17
GGCACGG	45	1.315423E-4	24.666666	20
TGATACA	45	1.315423E-4	24.666666	2
CCGTCTT	2245	0.0	24.22717	37
GCCGTCT	2275	0.0	23.98901	36
GACACAT	70	1.8988794E-7	23.785715	20
ACAGCCC	55	1.8878072E-5	23.545454	11
CGCCTCA	40	0.0019230873	23.125002	27
TACACAA	65	2.6560465E-6	22.76923	20
>>END_MODULE