FastQCFastQC Report
Fri 10 Feb 2017
ERR1630904.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630904.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170153
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT1858810.92428578984796No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG155839.15822818287071No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT125737.389232044101485TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC86945.109519079886925No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC43072.531251285607659RNA PCR Primer, Index 6 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC43012.5277250474572885No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG20701.2165521618778394No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA14870.873919354933501No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13300.7816494566654717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13130.7716584485727551No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT12890.757553495971273No Hit
ACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT10000.5877063583950914No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5690.33440491792680704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5080.29855483006470646No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3750.22038988439815932No Hit
ATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCT3420.20099557457112127RNA PCR Primer, Index 40 (95% over 21bp)
TCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT3290.1933553919119851RNA PCR Primer, Index 40 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC3000.17631190751852746No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2960.1739610820849471No Hit
CTGATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT2750.16161924855865015No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2690.1580930104082796No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2500.14692658959877286No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2450.1439880578067974No Hit
CTTTACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA2270.13340934335568577No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2130.12518145433815447No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2050.12047980347099375No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1980.1163658589622281No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG1960.11519044624543794No Hit
TCGCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT1950.11460273988704284TruSeq Adapter, Index 6 (95% over 21bp)
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG1910.11225191445346247No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1780.10461173179432628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCC200.001837532937.04
TCAGAGT308.270401E-637.03
TTCTACA200.001837532937.02
CTTATAC20450.036.638141
TTATACA22250.033.6741562
GGTATCA1650.033.6363641
TGATACA451.0707845E-732.888892
CGTCTTC5650.032.41592837
TCGCCGA401.5894839E-632.3751
GCCGAGC401.5894839E-632.3753
TATACAC24300.031.061733
TATGCTT303.5867156E-430.8333345
TAGGACC250.00548335629.5999984
AGTGGTT250.00548335629.59999828
ATTACGG250.00548335629.5999982
ATCAGCA250.00548335629.59999831
GATCAGC250.00548335629.59999830
GATTACG250.00548335629.5999981
TTACGGG250.00548335629.5999983
CGCCGAG453.9819297E-628.7777792
Produced by FastQC (version 0.11.5)