##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630904.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 170153 Sequences flagged as poor quality 0 Sequence length 43 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.12019182735538 31.0 31.0 34.0 28.0 34.0 2 31.263080874271978 31.0 31.0 34.0 28.0 34.0 3 30.738876187901475 31.0 31.0 34.0 27.0 34.0 4 34.82168401379935 37.0 35.0 37.0 32.0 37.0 5 35.00229793186133 35.0 35.0 37.0 32.0 37.0 6 35.18181283903311 36.0 35.0 37.0 32.0 37.0 7 35.14800796929822 37.0 35.0 37.0 32.0 37.0 8 35.17694075332201 37.0 35.0 37.0 32.0 37.0 9 37.01257103900607 39.0 37.0 39.0 33.0 39.0 10 36.79649491927853 39.0 37.0 39.0 32.0 39.0 11 36.81838110406517 39.0 37.0 39.0 32.0 39.0 12 36.68277373892908 39.0 35.0 39.0 32.0 39.0 13 36.80644478792616 39.0 37.0 39.0 32.0 39.0 14 37.57823253189776 39.0 37.0 41.0 32.0 41.0 15 37.6197833714363 39.0 37.0 41.0 32.0 41.0 16 37.60107667804858 39.0 37.0 41.0 32.0 41.0 17 37.55014604503006 39.0 36.0 40.0 32.0 41.0 18 37.70197998272143 39.0 37.0 41.0 32.0 41.0 19 37.59321316697326 39.0 37.0 41.0 32.0 41.0 20 37.67402866831616 39.0 37.0 41.0 32.0 41.0 21 37.53122190028974 39.0 37.0 41.0 32.0 41.0 22 37.36782190146515 39.0 36.0 40.0 31.0 41.0 23 37.46553983767551 39.0 36.0 40.0 32.0 41.0 24 37.248111993323654 39.0 36.0 40.0 31.0 41.0 25 37.19440738629351 39.0 36.0 40.0 31.0 41.0 26 36.78297767303545 39.0 35.0 40.0 30.0 41.0 27 36.83531880131411 39.0 35.0 40.0 30.0 41.0 28 36.882341187049306 39.0 35.0 40.0 31.0 41.0 29 36.69279413233972 39.0 35.0 40.0 30.0 41.0 30 36.69431629180796 39.0 35.0 40.0 30.0 41.0 31 36.34959712729132 38.0 35.0 40.0 30.0 41.0 32 36.3654593219044 38.0 35.0 40.0 30.0 41.0 33 35.99730830487855 38.0 34.0 40.0 28.0 41.0 34 36.00664695891345 38.0 34.0 40.0 28.0 41.0 35 36.22832392023649 38.0 35.0 40.0 29.0 41.0 36 35.920013164622425 38.0 34.0 40.0 28.0 41.0 37 36.03021398388509 38.0 34.0 40.0 28.0 41.0 38 36.055579390313426 38.0 35.0 40.0 28.0 41.0 39 35.784981751717574 38.0 34.0 40.0 28.0 41.0 40 35.346564562482 38.0 34.0 40.0 26.0 41.0 41 35.414773762437335 38.0 34.0 40.0 26.0 41.0 42 35.203422801831294 38.0 34.0 40.0 26.0 41.0 43 33.9443030684149 36.0 32.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 10.0 10 16.0 11 12.0 12 6.0 13 9.0 14 9.0 15 2.0 16 13.0 17 14.0 18 10.0 19 19.0 20 47.0 21 88.0 22 167.0 23 298.0 24 472.0 25 833.0 26 1310.0 27 1878.0 28 2770.0 29 3592.0 30 4584.0 31 5701.0 32 6996.0 33 8646.0 34 10995.0 35 14275.0 36 17998.0 37 25396.0 38 37158.0 39 26826.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.740986053728115 26.985125152069024 18.451041121813898 30.82284767238897 2 8.984267100785763 18.09724189406005 46.97948317102843 25.93900783412576 3 10.075637808325448 32.56304619959683 40.84911814660923 16.512197845468492 4 7.021327863746158 24.357490023684566 23.099798416719068 45.521383695850204 5 10.482330608334852 37.50859520549153 33.679100574189114 18.329973611984506 6 23.196182259495863 37.718406375438576 15.098764053528296 23.98664731153726 7 17.35731958884063 36.12513443782948 13.521360187595869 32.99618578573401 8 25.852027293083285 30.14228370936745 26.536705200613564 17.4689837969357 9 15.59361280729696 11.192867595634517 11.719452492756519 61.494067104312 10 13.646541641934023 27.867272396020056 27.477623080404108 31.008562881641815 11 25.12092058323979 17.04759833796642 25.324854689602887 32.506626389190906 12 14.493426504381352 28.32744647464341 16.45577803506256 40.72334898591268 13 35.46220166555982 12.65214248352953 25.49940347804623 26.386252372864423 14 22.044865503399883 26.89814461102655 16.09316321193279 34.96382667364078 15 28.18110759140303 24.657220266466062 13.390301669673764 33.77137047245714 16 35.513332118740195 19.390195882529255 13.637138340199703 31.459333658530852 17 14.912461137917052 42.00807508536435 14.146092046569853 28.93337173014875 18 29.430571309350995 13.983885091652807 15.355004025788555 41.23053957320764 19 23.833255951996144 26.585484828360357 15.952113685917968 33.62914553372553 20 23.672224409795888 21.918508636344935 16.080821378406494 38.32844557545268 21 29.069131898938018 23.738635228294534 18.545074139157112 28.64715873361034 22 16.45577803506256 41.28167002638802 23.70043431499885 18.56211762355057 23 24.956362802889164 19.745758229358284 16.17955604661687 39.118322921135686 24 29.030930985642335 33.162506685159826 18.59091523511193 19.21564709408591 25 27.65099645613066 28.523740398347368 19.304978460561966 24.520284684960007 26 24.86115437282916 26.129424694245767 25.335433404053997 23.673987528871073 27 25.432992659547583 34.07286383431382 24.549082296521366 15.945061209617226 28 13.475519091641052 25.511745311572525 27.334222728955705 33.67851286783072 29 15.590674275504988 22.668421949657073 28.623650479274538 33.1172532955634 30 30.127591050407577 15.882764335627348 28.60660699488108 25.383037619084003 31 17.42666893913125 35.98467261817306 23.166796941576113 23.421861501119583 32 29.71031953594706 20.272343126480287 34.893301910633376 15.124035426939283 33 13.677690078928965 17.597691489424225 40.537046070301436 28.187572361345374 34 16.249493103265884 42.8761173767139 21.33550392881701 19.53888559120321 35 27.682732599483995 15.285066969139539 43.011289839144766 14.020910592231697 36 22.305807126527302 17.582411124105953 29.373563792586673 30.738217956780073 37 19.075185274429487 26.38977861101479 30.4960829371213 24.038953177434426 38 18.21360775302228 15.09582552173632 34.15984437535629 32.5307223498851 39 30.875741244644527 18.517451940312544 21.918508636344935 28.688298178697995 40 14.845462613060011 14.191933142524668 50.84071394568418 20.12189029873114 41 22.74423606989004 22.293465293001006 20.091329568094597 34.87096906901436 42 25.04510646300682 14.51752246507555 39.82944761479374 20.607923457123885 43 24.217615910386534 14.345912208424183 37.32464311531387 24.11182876587542 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10.0 1 7.5 2 5.0 3 21.0 4 37.0 5 37.0 6 54.5 7 72.0 8 86.5 9 101.0 10 154.0 11 207.0 12 207.0 13 339.0 14 471.0 15 1181.0 16 1891.0 17 1950.0 18 2009.0 19 2009.0 20 1647.0 21 1285.0 22 1054.0 23 823.0 24 825.0 25 827.0 26 827.0 27 982.5 28 1138.0 29 1230.0 30 1322.0 31 1509.5 32 1697.0 33 1697.0 34 2107.5 35 2518.0 36 2890.0 37 3262.0 38 3723.0 39 4184.0 40 4184.0 41 4483.0 42 4782.0 43 5072.5 44 5363.0 45 6472.0 46 7581.0 47 7581.0 48 10421.0 49 13261.0 50 29224.0 51 45187.0 52 28477.5 53 11768.0 54 11768.0 55 21892.5 56 32017.0 57 25083.0 58 18149.0 59 11290.5 60 4432.0 61 4432.0 62 3529.5 63 2627.0 64 2033.5 65 1440.0 66 1171.0 67 902.0 68 902.0 69 649.5 70 397.0 71 317.0 72 237.0 73 150.5 74 64.0 75 64.0 76 54.0 77 44.0 78 32.0 79 20.0 80 13.0 81 6.0 82 6.0 83 8.0 84 10.0 85 6.0 86 2.0 87 2.0 88 2.0 89 2.0 90 2.0 91 2.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 170153.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.509776495271904 #Duplication Level Percentage of deduplicated Percentage of total 1 69.75471373717693 14.306535882411712 2 11.914722906756834 4.887366076413581 3 5.2897014155539 3.254717812792016 4 2.799587368903662 2.2967564486080176 5 1.8654364146942517 1.9129841965760226 6 1.361109519170153 1.6749631214260108 7 1.060232678090435 1.5221594682432869 8 0.7478938621124419 1.227130876328951 9 0.5988881884348674 1.105475660141167 >10 4.154965900624678 16.02734009979254 >50 0.2922803599060118 4.064577174660452 >100 0.1203507364318872 4.457752728426769 >500 0.00859648117370623 1.220666106386605 >1k 0.0200584560719812 9.460309251085787 >5k 0.0028654937245687433 5.109519079886925 >10k+ 0.00859648117370623 27.471746016820152 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT 18588 10.92428578984796 No Hit ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG 15583 9.15822818287071 No Hit TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT 12573 7.389232044101485 TruSeq Adapter, Index 6 (95% over 21bp) ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC 8694 5.109519079886925 No Hit CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC 4307 2.531251285607659 RNA PCR Primer, Index 6 (95% over 22bp) CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC 4301 2.5277250474572885 No Hit CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG 2070 1.2165521618778394 No Hit TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA 1487 0.873919354933501 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 1330 0.7816494566654717 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 1313 0.7716584485727551 No Hit TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT 1289 0.757553495971273 No Hit ACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT 1000 0.5877063583950914 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 569 0.33440491792680704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 508 0.29855483006470646 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 375 0.22038988439815932 No Hit ATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCT 342 0.20099557457112127 RNA PCR Primer, Index 40 (95% over 21bp) TCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT 329 0.1933553919119851 RNA PCR Primer, Index 40 (95% over 24bp) GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC 300 0.17631190751852746 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT 296 0.1739610820849471 No Hit CTGATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT 275 0.16161924855865015 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 269 0.1580930104082796 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 250 0.14692658959877286 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC 245 0.1439880578067974 No Hit CTTTACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA 227 0.13340934335568577 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 213 0.12518145433815447 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG 205 0.12047980347099375 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 198 0.1163658589622281 No Hit GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG 196 0.11519044624543794 No Hit TCGCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT 195 0.11460273988704284 TruSeq Adapter, Index 6 (95% over 21bp) GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG 191 0.11225191445346247 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 178 0.10461173179432628 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 5.877063583950915E-4 0.0 16 0.0 0.0 0.0 5.877063583950915E-4 0.0 17 0.0 0.0 0.0 5.877063583950915E-4 0.0 18 0.0 0.0 0.0 5.877063583950915E-4 0.0 19 0.0 0.0 0.0 5.877063583950915E-4 0.0 20 0.0 0.0 0.0 0.001175412716790183 0.0 21 0.0 0.0 0.0 0.002350825433580366 0.0 22 0.0 0.0 0.0 0.0029385317919754573 0.0 23 0.0 0.0 0.0 0.0029385317919754573 0.0 24 0.0 0.0 0.0 0.00411394450876564 0.0 25 0.0 0.0 0.0 0.004701650867160732 0.0 26 0.0 0.0 0.0 0.004701650867160732 0.0 27 0.0 0.0 0.0 0.006464769942346006 0.0 28 0.0 0.0 0.0 0.01175412716790183 0.0 29 0.0 0.0 0.0 0.03173614335333494 0.0 30 0.0 0.0 0.0 0.0617091676314846 0.0 31 0.0 0.0 0.0 0.11107650173667229 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGAGCC 20 0.0018375329 37.0 4 TCAGAGT 30 8.270401E-6 37.0 3 TTCTACA 20 0.0018375329 37.0 2 CTTATAC 2045 0.0 36.63814 1 TTATACA 2225 0.0 33.674156 2 GGTATCA 165 0.0 33.636364 1 TGATACA 45 1.0707845E-7 32.88889 2 CGTCTTC 565 0.0 32.415928 37 TCGCCGA 40 1.5894839E-6 32.375 1 GCCGAGC 40 1.5894839E-6 32.375 3 TATACAC 2430 0.0 31.06173 3 TATGCTT 30 3.5867156E-4 30.833334 5 TAGGACC 25 0.005483356 29.599998 4 AGTGGTT 25 0.005483356 29.599998 28 ATTACGG 25 0.005483356 29.599998 2 ATCAGCA 25 0.005483356 29.599998 31 GATCAGC 25 0.005483356 29.599998 30 GATTACG 25 0.005483356 29.599998 1 TTACGGG 25 0.005483356 29.599998 3 CGCCGAG 45 3.9819297E-6 28.777779 2 >>END_MODULE